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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Trimeresurus gramineus (Bamboo pit viper) (Indian green tree viper)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom metalloproteinase graminelysin: cleaves the alpha chain of fibrinogen (FGA) preferentially and cleaves the beta chain (FGB) either on longer incubation or at high concentrations. Induces apoptosis of endothelial cells (prior to cell detachment).3 Publications
Disintegrin: inhibits platelet aggregation induced by ADP, thrombin, platelet-activating factor and collagen. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3) (By similarity).By similarity

Miscellaneous

The metalloproteinase does not bind von Willebrand factor (vWF).1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi169Zinc; catalyticBy similarity1
Active sitei170PROSITE-ProRule annotation1
Metal bindingi173Zinc; catalyticBy similarity1
Metal bindingi179Zinc; catalyticBy similarity1
Metal bindingi237Calcium; via carbonyl oxygenBy similarity1
Metal bindingi240CalciumBy similarity1
Metal bindingi242Calcium; via carbonyl oxygenBy similarity1
Metal bindingi244CalciumBy similarity1
Metal bindingi247CalciumBy similarity1
Metal bindingi250CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
Biological processApoptosis
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
Alternative name(s):
Graminelysin I
OrganismiTrimeresurus gramineus (Bamboo pit viper) (Indian green tree viper)
Taxonomic identifieri8767 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeTrimeresurus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000322605‹1 – 26Add BLAST›26
ChainiPRO_000032260627 – 227Snake venom metalloproteinase graminelysinAdd BLAST201
PropeptideiPRO_0000322607228 – 2431 PublicationAdd BLAST16
ChainiPRO_0000322608244 – 435Disintegrin-likeAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27Pyrrolidone carboxylic acidBy similarity1
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi144 ↔ 222By similarity
Disulfide bondi184 ↔ 206By similarity
Disulfide bondi186 ↔ 189By similarity
Disulfide bondi249 ↔ 264By similarity
Disulfide bondi251 ↔ 259By similarity
Disulfide bondi258 ↔ 281By similarity
Disulfide bondi272 ↔ 278By similarity
Disulfide bondi277 ↔ 303By similarity
Disulfide bondi290 ↔ 310By similarity

Post-translational modificationi

The N-terminus of the metalloproteinase is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP0C6E8.
SMRiP0C6E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 227Peptidase M12BPROSITE-ProRule annotationAdd BLAST195
Domaini235 – 318DisintegrinPROSITE-ProRule annotationAdd BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 298D/ECD-tripeptide3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi319 – 435Cys-richAdd BLAST117

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom_sf.
IPR001590. Peptidase_M12B.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C6E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KMCGVTQNWE SYESTKKASQ LNLTPEQQRF PQRYIKLGIF VDHGMYTKYS
60 70 80 90 100
GNSERITKRV HQMINNINMM CRALNIVTTL SLLEIWSEKD LITVQASAPT
110 120 130 140 150
TLTLFGAWRE TVLLNRTSHD HAQLLTATIF NGNVIGRAPV GGMCDPKRSV
160 170 180 190 200
AIVRDHNAIV FVVAVTMTHE MGHNLGNHHD EDKCNCNTCI MSKVLSRQPS
210 220 230 240 250
KYFSECSKDY YQTFLTNHNF QCILNAPLRT DTVSTPVSGN ELLEAGEDCD
260 270 280 290 300
CGSPANPCCD AATCKLRPGA QCGEGLCCDQ CRFTSAGTEC RAARSECDIA
310 320 330 340 350
ESCAGQSADC PTDDFHRNGQ PCLNNHGYCY NGNCPIMFYQ CIALFGSNAT
360 370 380 390 400
VGQDGCFDAN DIGHKYFHCR KDNEKYIPCA PQDVKCGRLF CTYIYDIDLC
410 420 430
RYDDSANGMV AQGTKCADGK VCNSNRQCAD VNTAY
Length:435
Mass (Da):48,204
Last modified:February 26, 2008 - v1
Checksum:iFC4F87D31EA32E5E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Mass spectrometryi

Molecular mass is 27020 Da from positions 27 - 227. Determined by MALDI. 1 Publication

Similar proteinsi

Entry informationi

Entry nameiVM3G1_TRIGA
AccessioniPrimary (citable) accession number: P0C6E8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 22, 2017
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families