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Protein

Vibriobactin-specific isochorismatase

Gene

vibB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the catechol siderophore vibriobactin. Vibriobactin is a chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm.

Catalytic activityi

Isochorismate + H2O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.

Cofactori

pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity

Pathwayi: vibriobactin biosynthesis

This protein is involved in the pathway vibriobactin biosynthesis, which is part of Siderophore biosynthesis.
View all proteins of this organism that are known to be involved in the pathway vibriobactin biosynthesis and in Siderophore biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:VC0771-MONOMER.
VCHO:VC0771-MONOMER.
UniPathwayiUPA00022.

Names & Taxonomyi

Protein namesi
Recommended name:
Vibriobactin-specific isochorismatase (EC:3.3.2.1)
Alternative name(s):
2,3 dihydro-2,3 dihydroxybenzoate synthase
Isochorismate lyase-ArCP
Gene namesi
Name:vibB
Ordered Locus Names:VC_0771
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293Vibriobactin-specific isochorismatasePRO_0000201827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei248 – 2481O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0771.

Structurei

Secondary structure

1
293
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni26 – 283Combined sources
Beta strandi29 – 346Combined sources
Helixi38 – 414Combined sources
Helixi51 – 6818Combined sources
Beta strandi72 – 765Combined sources
Helixi83 – 864Combined sources
Helixi89 – 935Combined sources
Helixi105 – 1073Combined sources
Beta strandi113 – 1175Combined sources
Beta strandi120 – 1223Combined sources
Turni123 – 1264Combined sources
Helixi129 – 1368Combined sources
Beta strandi140 – 1467Combined sources
Turni148 – 1503Combined sources
Helixi151 – 16111Combined sources
Beta strandi165 – 17410Combined sources
Helixi178 – 19114Combined sources
Beta strandi194 – 1963Combined sources
Helixi198 – 2058Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TB4X-ray1.35A1-215[»]
3TG2X-ray1.10A1-215[»]
ProteinModelPortaliP0C6D3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini213 – 28472Acyl carrierPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the isochorismatase family.Curated
Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105WQI. Bacteria.
COG1535. LUCA.
COG3433. LUCA.
KOiK01252.
OMAiRDIKPFF.
OrthoDBiEOG6G20K9.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.50.850. 1 hit.
InterProiIPR016291. Isochorismatase.
IPR000868. Isochorismatase-like.
IPR009081. PP-bd_ACP.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF001111. Isochorismatase. 1 hit.
PRINTSiPR01398. ISCHRISMTASE.
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52499. SSF52499. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C6D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPKIASYP LPVSLPTNKV DWRIDASRAV LLIHDMQEYF VHYFDSQAEP
60 70 80 90 100
IPSLIKHIQQ LKAHAKQAGI PVVYTAQPAN QDPAERALLS DFWGPGLSEE
110 120 130 140 150
TAIIAPLAPE SGDVQLTKWR YSAFKKSPLL DWLRETGRDQ LIITGVYAHI
160 170 180 190 200
GILSTALDAF MFDIQPFVIG DGVADFSLSD HEFSLRYISG RTGAVKSTQQ
210 220 230 240 250
ACLEIAAQHS KLTGLSLRTM QHDVAAALNL SVDEVDVQEN LLFLGLDSIR
260 270 280 290
AIQLLEKWKA QGADISFAQL MEHVTLQQWW QTIQANLHQP CSA
Length:293
Mass (Da):32,553
Last modified:February 26, 2008 - v1
Checksum:iA64283991E17A3F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52150 Genomic DNA. Translation: AAC45926.1.
AE003852 Genomic DNA. Translation: AAF93936.1.
PIRiG82282.
RefSeqiNP_230420.1. NC_002505.1.
WP_000997093.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93936; AAF93936; VC_0771.
GeneIDi2615314.
KEGGivch:VC0771.
PATRICi20080653. VBIVibCho83274_0735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52150 Genomic DNA. Translation: AAC45926.1.
AE003852 Genomic DNA. Translation: AAF93936.1.
PIRiG82282.
RefSeqiNP_230420.1. NC_002505.1.
WP_000997093.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TB4X-ray1.35A1-215[»]
3TG2X-ray1.10A1-215[»]
ProteinModelPortaliP0C6D3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0771.

Protocols and materials databases

DNASUi2615314.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93936; AAF93936; VC_0771.
GeneIDi2615314.
KEGGivch:VC0771.
PATRICi20080653. VBIVibCho83274_0735.

Phylogenomic databases

eggNOGiENOG4105WQI. Bacteria.
COG1535. LUCA.
COG3433. LUCA.
KOiK01252.
OMAiRDIKPFF.
OrthoDBiEOG6G20K9.

Enzyme and pathway databases

UniPathwayiUPA00022.
BioCyciMetaCyc:VC0771-MONOMER.
VCHO:VC0771-MONOMER.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.50.850. 1 hit.
InterProiIPR016291. Isochorismatase.
IPR000868. Isochorismatase-like.
IPR009081. PP-bd_ACP.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF001111. Isochorismatase. 1 hit.
PRINTSiPR01398. ISCHRISMTASE.
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52499. SSF52499. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis."
    Wyckoff E.E., Stoebner J.A., Reed K.E., Payne S.M.
    J. Bacteriol. 179:7055-7062(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: El Tor Lou15.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiVIBB_VIBCH
AccessioniPrimary (citable) accession number: P0C6D3
Secondary accession number(s): O07900, Q9JQ11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: May 11, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.