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Protein

Vibriobactin-specific isochorismatase

Gene

vibB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the catechol siderophore vibriobactin. Vibriobactin is a chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm.

Catalytic activityi

Isochorismate + H2O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.

Cofactori

pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity

Pathwayi: vibriobactin biosynthesis

This protein is involved in the pathway vibriobactin biosynthesis, which is part of Siderophore biosynthesis.
View all proteins of this organism that are known to be involved in the pathway vibriobactin biosynthesis and in Siderophore biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:VC0771-MONOMER.
VCHO:VC0771-MONOMER.
UniPathwayiUPA00022.

Names & Taxonomyi

Protein namesi
Recommended name:
Vibriobactin-specific isochorismatase (EC:3.3.2.1)
Alternative name(s):
2,3 dihydro-2,3 dihydroxybenzoate synthase
Isochorismate lyase-ArCP
Gene namesi
Name:vibB
Ordered Locus Names:VC_0771
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002018271 – 293Vibriobactin-specific isochorismataseAdd BLAST293

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei248O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0771.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni26 – 28Combined sources3
Beta strandi29 – 34Combined sources6
Helixi38 – 41Combined sources4
Helixi51 – 68Combined sources18
Beta strandi72 – 76Combined sources5
Helixi83 – 86Combined sources4
Helixi89 – 93Combined sources5
Helixi105 – 107Combined sources3
Beta strandi113 – 117Combined sources5
Beta strandi120 – 122Combined sources3
Turni123 – 126Combined sources4
Helixi129 – 136Combined sources8
Beta strandi140 – 146Combined sources7
Turni148 – 150Combined sources3
Helixi151 – 161Combined sources11
Beta strandi165 – 174Combined sources10
Helixi178 – 191Combined sources14
Beta strandi194 – 196Combined sources3
Helixi198 – 205Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TB4X-ray1.35A1-215[»]
3TG2X-ray1.10A1-215[»]
ProteinModelPortaliP0C6D3.
SMRiP0C6D3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini213 – 284Acyl carrierPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the isochorismatase family.Curated
Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105WQI. Bacteria.
COG1535. LUCA.
COG3433. LUCA.
KOiK01252.
OMAiRDIKPFF.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.50.850. 1 hit.
InterProiIPR016291. Isochorismatase.
IPR000868. Isochorismatase-like.
IPR009081. PP-bd_ACP.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF001111. Isochorismatase. 1 hit.
PRINTSiPR01398. ISCHRISMTASE.
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52499. SSF52499. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C6D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPKIASYP LPVSLPTNKV DWRIDASRAV LLIHDMQEYF VHYFDSQAEP
60 70 80 90 100
IPSLIKHIQQ LKAHAKQAGI PVVYTAQPAN QDPAERALLS DFWGPGLSEE
110 120 130 140 150
TAIIAPLAPE SGDVQLTKWR YSAFKKSPLL DWLRETGRDQ LIITGVYAHI
160 170 180 190 200
GILSTALDAF MFDIQPFVIG DGVADFSLSD HEFSLRYISG RTGAVKSTQQ
210 220 230 240 250
ACLEIAAQHS KLTGLSLRTM QHDVAAALNL SVDEVDVQEN LLFLGLDSIR
260 270 280 290
AIQLLEKWKA QGADISFAQL MEHVTLQQWW QTIQANLHQP CSA
Length:293
Mass (Da):32,553
Last modified:February 26, 2008 - v1
Checksum:iA64283991E17A3F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52150 Genomic DNA. Translation: AAC45926.1.
AE003852 Genomic DNA. Translation: AAF93936.1.
PIRiG82282.
RefSeqiNP_230420.1. NC_002505.1.
WP_000997093.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93936; AAF93936; VC_0771.
GeneIDi2615314.
KEGGivch:VC0771.
PATRICi20080653. VBIVibCho83274_0735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52150 Genomic DNA. Translation: AAC45926.1.
AE003852 Genomic DNA. Translation: AAF93936.1.
PIRiG82282.
RefSeqiNP_230420.1. NC_002505.1.
WP_000997093.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TB4X-ray1.35A1-215[»]
3TG2X-ray1.10A1-215[»]
ProteinModelPortaliP0C6D3.
SMRiP0C6D3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0771.

Protocols and materials databases

DNASUi2615314.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93936; AAF93936; VC_0771.
GeneIDi2615314.
KEGGivch:VC0771.
PATRICi20080653. VBIVibCho83274_0735.

Phylogenomic databases

eggNOGiENOG4105WQI. Bacteria.
COG1535. LUCA.
COG3433. LUCA.
KOiK01252.
OMAiRDIKPFF.

Enzyme and pathway databases

UniPathwayiUPA00022.
BioCyciMetaCyc:VC0771-MONOMER.
VCHO:VC0771-MONOMER.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.50.850. 1 hit.
InterProiIPR016291. Isochorismatase.
IPR000868. Isochorismatase-like.
IPR009081. PP-bd_ACP.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF001111. Isochorismatase. 1 hit.
PRINTSiPR01398. ISCHRISMTASE.
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52499. SSF52499. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVIBB_VIBCH
AccessioniPrimary (citable) accession number: P0C6D3
Secondary accession number(s): O07900, Q9JQ11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.