P0C6D3 (VIBB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vibriobactin-specific isochorismatase
EC=3.3.2.1

Alternative name(s):
2,3 dihydro-2,3 dihydroxybenzoate synthase
Isochorismate lyase-ArCP

Gene names

Name:	vibB
Ordered Locus Names:	VC_0771

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of the catechol siderophore vibriobactin. Vibriobactin is a chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm.
Catalytic activity	Isochorismate + H2O = 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.
Cofactor	Binds 1 phosphopantetheine covalently By similarity.
Pathway	Siderophore biosynthesis; vibriobactin biosynthesis.
Sequence similarities	Belongs to the isochorismatase family. Contains 1 acyl carrier domain.

Ontologies

Keywords
Ligand	Phosphopantetheine
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro isochorismatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Vibriobactin-specific isochorismatase		PRO_0000201827
Regions
	Domain	213 – 284	72	Acyl carrier
Amino acid modifications
	Modified residue	248	1	O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C6D3 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: A64283991E17A3F5	FASTA	293	32,553
	10 20 30 40 50 60 MAIPKIASYP LPVSLPTNKV DWRIDASRAV LLIHDMQEYF VHYFDSQAEP IPSLIKHIQQ 70 80 90 100 110 120 LKAHAKQAGI PVVYTAQPAN QDPAERALLS DFWGPGLSEE TAIIAPLAPE SGDVQLTKWR 130 140 150 160 170 180 YSAFKKSPLL DWLRETGRDQ LIITGVYAHI GILSTALDAF MFDIQPFVIG DGVADFSLSD 190 200 210 220 230 240 HEFSLRYISG RTGAVKSTQQ ACLEIAAQHS KLTGLSLRTM QHDVAAALNL SVDEVDVQEN 250 260 270 280 290 LLFLGLDSIR AIQLLEKWKA QGADISFAQL MEHVTLQQWW QTIQANLHQP CSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis." Wyckoff E.E., Stoebner J.A., Reed K.E., Payne S.M. J. Bacteriol. 179:7055-7062(1997) [PubMed: 9371453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor Lou15.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52150 Genomic DNA. Translation: AAC45926.1. AE003852 Genomic DNA. Translation: AAF93936.1.
PIR	G82282.
RefSeq	NP_230420.1. NC_002505.1.
3D structure databases
ProteinModelPortal	P0C6D3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615314.
GenomeReviews	Gene locus VC_0771 in contig AE003852_GR.
KEGG	vch:VC0771.
PATRIC	20080653. VBIVibCho83274_0735.
TIGR	VC_0771.
Phylogenomic databases
HOGENOM	HBG289118.
OMA	HDMQNYF.
PhylomeDB	P0C6D3.
ProtClustDB	CLSK874104.
Enzyme and pathway databases
BioCyc	MetaCyc:VC0771-MONOMER. VCHO:VC0771-MONOMER.
Family and domain databases
InterPro	IPR009081. Acyl_carrier_prot-like. IPR016291. Isochorismatase. IPR000868. Isochorismatase-like. IPR006163. Phsphopanteth-bd. [Graphical view]
Gene3D	G3DSA:1.10.1200.10. ACP_like. 1 hit. G3DSA:3.40.50.850. Isochorismatase_hydro. 1 hit.
KO	K04777.
Pfam	PF00857. Isochorismatase. 1 hit. PF00550. PP-binding. 1 hit. [Graphical view]
PIRSF	PIRSF001111. Isochorismatase. 1 hit.
PRINTS	PR01398. ISCHRISMTASE.
SUPFAM	SSF47336. ACP_like. 1 hit. SSF52499. Iscrsm_hydrolase. 1 hit.
PROSITE	PS50075. ACP_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VIBB_VIBCH

Accession

Primary (citable) accession number: P0C6D3
Secondary accession number(s): O07900, Q9JQ11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program