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Protein

Pre T-cell antigen receptor alpha

Gene

Ptcra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. Isoform 1 acts to retain most TCRB intracellularly, while isoform 2 permits higher levels of cell surface TCRB expression and facilitates signaling from the CD3-TCRB complex.1 Publication

GO - Biological processi

  • negative regulation of thymocyte apoptotic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Pre T-cell antigen receptor alpha
Short name:
pT-alpha
Short name:
pTa
Alternative name(s):
gp33
pT-alpha-TCR
Gene namesi
Name:Ptcra
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104857. Ptcra.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 146ExtracellularSequence analysisAdd BLAST130
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Topological domaini168 – 199CytoplasmicSequence analysisAdd BLAST32

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000031910917 – 199Pre T-cell antigen receptor alphaAdd BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 107By similarity
Glycosylationi67N-linked (GlcNAc...)Sequence analysis1
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi135Interchain (with TCRB)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP0C6B2.

PTM databases

PhosphoSitePlusiP0C6B2.

Expressioni

Tissue specificityi

Isoform 1 is expressed at higher levels than isoform 2 in the thymus while only isoform 2 is expressed in polyclonal beta-only cells. Isoform 1 shows a predominant expression in immature thymocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000036858.
CleanExiMM_PTCRA.

Interactioni

Subunit structurei

Interacts with RHBDD1 (By similarity). Heterodimer with TCRB; disulfide linked. This heterodimer assembles with CD3 proteins into a signaling-competent pre-T-cell receptor complex.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliP0C6B2.
SMRiP0C6B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi160 – 163Poly-Leu4

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000204058.
HOVERGENiHBG108303.
InParanoidiP0C6B2.
KOiK06056.
TreeFamiTF337868.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR027834. PTCRA.
[Graphical view]
PfamiPF15028. PTCRA. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P0C6B2-1) [UniParc]FASTAAdd to basket
Also known as: pTalpha-a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARTWLLLLL GVRCQALPSG IAGTPFPSLA PPITLLVDGR QHMLVVCLVL
60 70 80 90 100
DAAPPGLDNP VWFSAGNGSA LDAFTYGPSL APDGTWTSLA QLSLPSEELE
110 120 130 140 150
AWEPLVCHTR PGAGGQNRST HPLQLSGESS TARSCFPEPL GGTQRQVLWL
160 170 180 190
SLLRLLLFKL LLLDVLLTCS HLRLHVLAGQ HLQPPPSRKS LPPTHRIWT
Length:199
Mass (Da):21,491
Last modified:February 26, 2008 - v1
Checksum:i5141E9361BF494BA
GO
Isoform 2 (identifier: P0C6B2-2) [UniParc]FASTAAdd to basket
Also known as: pTalpha-b

The sequence of this isoform differs from the canonical sequence as follows:
     20-126: Missing.

Show »
Length:92
Mass (Da):10,368
Checksum:iC35CFA1E43A2E716
GO

Sequence cautioni

The sequence AAA58963 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI19063 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI20747 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03144720 – 126Missing in isoform 2. 1 PublicationAdd BLAST107

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16958 mRNA. Translation: AAA58963.1. Different initiation.
U27268 Genomic DNA. Translation: AAA84398.1.
BC119062 mRNA. Translation: AAI19063.1. Different initiation.
BC120746 mRNA. Translation: AAI20747.1. Different initiation.
RefSeqiNP_035325.1. NM_011195.2.
UniGeneiMm.215173.

Genome annotation databases

GeneIDi19208.
KEGGimmu:19208.
UCSCiuc008cuh.1. mouse. [P0C6B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16958 mRNA. Translation: AAA58963.1. Different initiation.
U27268 Genomic DNA. Translation: AAA84398.1.
BC119062 mRNA. Translation: AAI19063.1. Different initiation.
BC120746 mRNA. Translation: AAI20747.1. Different initiation.
RefSeqiNP_035325.1. NM_011195.2.
UniGeneiMm.215173.

3D structure databases

ProteinModelPortaliP0C6B2.
SMRiP0C6B2.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSitePlusiP0C6B2.

Proteomic databases

PRIDEiP0C6B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19208.
KEGGimmu:19208.
UCSCiuc008cuh.1. mouse. [P0C6B2-1]

Organism-specific databases

CTDi171558.
MGIiMGI:104857. Ptcra.

Phylogenomic databases

HOGENOMiHOG000204058.
HOVERGENiHBG108303.
InParanoidiP0C6B2.
KOiK06056.
TreeFamiTF337868.

Miscellaneous databases

PROiP0C6B2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036858.
CleanExiMM_PTCRA.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR027834. PTCRA.
[Graphical view]
PfamiPF15028. PTCRA. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTCRA_MOUSE
AccessioniPrimary (citable) accession number: P0C6B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 30, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.