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P0C661

- CDK8_CAEBR

UniProt

P0C661 - CDK8_CAEBR

Protein

Cyclin-dependent kinase 8

Gene

cdk-8

Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 2 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571ATPPROSITE-ProRule annotation
    Active sitei155 – 1551Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: UniProtKB
    2. protein phosphorylation Source: UniProtKB
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Cell division protein kinase 8
    Mediator complex subunit cdk-8
    Mediator of RNA polymerase II transcription subunit cdk-8
    Gene namesi
    Name:cdk-8
    ORF Names:CBG12346
    OrganismiCaenorhabditis briggsae
    Taxonomic identifieri6238 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000008549: Chromosome I, UP000008549: Unassembled WGS sequence

    Organism-specific databases

    WormBaseiCBG12346; CBP37605; WBGene00033308; Cbr-cdk-8.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 612612Cyclin-dependent kinase 8PRO_0000312930Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the Mediator complex.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C661.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 345323Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi371 – 609239Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    OrthoDBiEOG76739K.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C661-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDENFKKKL AVSRERVEDL FYFENSKEIG RGTYGLVYKA VPKHSNGRFP    50
    NKEYALKMIE GQGFSMSACR EIALFRELRH PNLICLQRVF LTNEKKVWLL 100
    LDYAEHDLWH VIKHHRTAKT KKVPIMVPRN MVKNILFQIL SGMHYLHSNW 150
    VLHRDLKPAN ILLMGDGGPD MRGRVKIADL GFSRIFANPL KPMAELDPVV 200
    VTFWYRAPEL LLGAKHYTKA IDVWAIGCIF AELLTAEPLF FCKEEDIKAQ 250
    NPYHYDQVKR IFHLLGYPSD TDWPDMKKMP DHQRLLNDAR NEGTPIQTFP 300
    NSLQRYFDKW KINSQSSPYR LLVKLLTVDP LKRVSCEEAM NDIYFRKMER 350
    PPRETDDVFN RYPIPYAKKE QQITIPIDQF QQQQQQQQQQ QQQQQQQQQQ 400
    QQQQQMQQPQ IGPPQMMGQP QMGQPQMGQP QMGQPQMGQP QMGQPQMVPS 450
    QMGQPPMGGP HPGVVAPDGH AHQMMQQQHQ SQHHMQYQQM HDSMQGGMDD 500
    GGPQAKMMRM GNVPVGRYGP MGPPYGPQDF HAPQGPPMMQ MMPQPGPSGY 550
    YQQRPGQPPG PGPQGYMNPQ MGMTMGMRPQ GVPQQAYMPG RGMPPPQGPN 600
    PQQQQQWQQY HR 612
    Length:612
    Mass (Da):70,642
    Last modified:November 24, 2009 - v2
    Checksum:iCE8F21A8F5A384CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE600913 Genomic DNA. Translation: CAP31338.3.

    Genome annotation databases

    EnsemblMetazoaiCBG12346; CBG12346; CBG12346.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE600913 Genomic DNA. Translation: CAP31338.3 .

    3D structure databases

    ProteinModelPortali P0C661.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai CBG12346 ; CBG12346 ; CBG12346 .

    Organism-specific databases

    WormBasei CBG12346 ; CBP37605 ; WBGene00033308 ; Cbr-cdk-8.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    OrthoDBi EOG76739K.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AF16.

    Entry informationi

    Entry nameiCDK8_CAEBR
    AccessioniPrimary (citable) accession number: P0C661
    Secondary accession number(s): A8XFM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3