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P0C661 (CDK8_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 8

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk-8
Mediator of RNA polymerase II transcription subunit cdk-8
Gene names
Name:cdk-8
ORF Names:CBG12346
OrganismCaenorhabditis briggsae [Reference proteome]
Taxonomic identifier6238 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the Mediator complex By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 612612Cyclin-dependent kinase 8
PRO_0000312930

Regions

Domain23 – 345323Protein kinase
Nucleotide binding29 – 379ATP By similarity
Compositional bias371 – 609239Gln-rich

Sites

Active site1551Proton acceptor By similarity
Binding site571ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C661 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: CE8F21A8F5A384CC

FASTA61270,642
        10         20         30         40         50         60 
MIDENFKKKL AVSRERVEDL FYFENSKEIG RGTYGLVYKA VPKHSNGRFP NKEYALKMIE 

        70         80         90        100        110        120 
GQGFSMSACR EIALFRELRH PNLICLQRVF LTNEKKVWLL LDYAEHDLWH VIKHHRTAKT 

       130        140        150        160        170        180 
KKVPIMVPRN MVKNILFQIL SGMHYLHSNW VLHRDLKPAN ILLMGDGGPD MRGRVKIADL 

       190        200        210        220        230        240 
GFSRIFANPL KPMAELDPVV VTFWYRAPEL LLGAKHYTKA IDVWAIGCIF AELLTAEPLF 

       250        260        270        280        290        300 
FCKEEDIKAQ NPYHYDQVKR IFHLLGYPSD TDWPDMKKMP DHQRLLNDAR NEGTPIQTFP 

       310        320        330        340        350        360 
NSLQRYFDKW KINSQSSPYR LLVKLLTVDP LKRVSCEEAM NDIYFRKMER PPRETDDVFN 

       370        380        390        400        410        420 
RYPIPYAKKE QQITIPIDQF QQQQQQQQQQ QQQQQQQQQQ QQQQQMQQPQ IGPPQMMGQP 

       430        440        450        460        470        480 
QMGQPQMGQP QMGQPQMGQP QMGQPQMVPS QMGQPPMGGP HPGVVAPDGH AHQMMQQQHQ 

       490        500        510        520        530        540 
SQHHMQYQQM HDSMQGGMDD GGPQAKMMRM GNVPVGRYGP MGPPYGPQDF HAPQGPPMMQ 

       550        560        570        580        590        600 
MMPQPGPSGY YQQRPGQPPG PGPQGYMNPQ MGMTMGMRPQ GVPQQAYMPG RGMPPPQGPN 

       610 
PQQQQQWQQY HR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HE600913 Genomic DNA. Translation: CAP31338.3.

3D structure databases

ProteinModelPortalP0C661.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaCBG12346; CBG12346; CBG12346.

Organism-specific databases

WormBaseCBG12346; CBP37605; WBGene00033308; Cbr-cdk-8.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
OrthoDBEOG76739K.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK8_CAEBR
AccessionPrimary (citable) accession number: P0C661
Secondary accession number(s): A8XFM1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 24, 2009
Last modified: February 19, 2014
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families