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P0C661

- CDK8_CAEBR

UniProt

P0C661 - CDK8_CAEBR

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Protein

Cyclin-dependent kinase 8

Gene

cdk-8

Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571ATPPROSITE-ProRule annotation
Active sitei155 – 1551Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: UniProtKB
  2. protein phosphorylation Source: UniProtKB
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk-8
Mediator of RNA polymerase II transcription subunit cdk-8
Gene namesi
Name:cdk-8
ORF Names:CBG12346
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000008549: Chromosome I, UP000008549: Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG12346; CBP37605; WBGene00033308; Cbr-cdk-8.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Cyclin-dependent kinase 8PRO_0000312930Add
BLAST

Interactioni

Subunit structurei

Component of the Mediator complex.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 345323Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi371 – 609239Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP0C661.
OrthoDBiEOG76739K.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDENFKKKL AVSRERVEDL FYFENSKEIG RGTYGLVYKA VPKHSNGRFP
60 70 80 90 100
NKEYALKMIE GQGFSMSACR EIALFRELRH PNLICLQRVF LTNEKKVWLL
110 120 130 140 150
LDYAEHDLWH VIKHHRTAKT KKVPIMVPRN MVKNILFQIL SGMHYLHSNW
160 170 180 190 200
VLHRDLKPAN ILLMGDGGPD MRGRVKIADL GFSRIFANPL KPMAELDPVV
210 220 230 240 250
VTFWYRAPEL LLGAKHYTKA IDVWAIGCIF AELLTAEPLF FCKEEDIKAQ
260 270 280 290 300
NPYHYDQVKR IFHLLGYPSD TDWPDMKKMP DHQRLLNDAR NEGTPIQTFP
310 320 330 340 350
NSLQRYFDKW KINSQSSPYR LLVKLLTVDP LKRVSCEEAM NDIYFRKMER
360 370 380 390 400
PPRETDDVFN RYPIPYAKKE QQITIPIDQF QQQQQQQQQQ QQQQQQQQQQ
410 420 430 440 450
QQQQQMQQPQ IGPPQMMGQP QMGQPQMGQP QMGQPQMGQP QMGQPQMVPS
460 470 480 490 500
QMGQPPMGGP HPGVVAPDGH AHQMMQQQHQ SQHHMQYQQM HDSMQGGMDD
510 520 530 540 550
GGPQAKMMRM GNVPVGRYGP MGPPYGPQDF HAPQGPPMMQ MMPQPGPSGY
560 570 580 590 600
YQQRPGQPPG PGPQGYMNPQ MGMTMGMRPQ GVPQQAYMPG RGMPPPQGPN
610
PQQQQQWQQY HR
Length:612
Mass (Da):70,642
Last modified:November 24, 2009 - v2
Checksum:iCE8F21A8F5A384CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600913 Genomic DNA. Translation: CAP31338.3.

Genome annotation databases

EnsemblMetazoaiCBG12346; CBG12346; CBG12346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600913 Genomic DNA. Translation: CAP31338.3 .

3D structure databases

ProteinModelPortali P0C661.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai CBG12346 ; CBG12346 ; CBG12346 .

Organism-specific databases

WormBasei CBG12346 ; CBP37605 ; WBGene00033308 ; Cbr-cdk-8.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi P0C661.
OrthoDBi EOG76739K.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AF16.

Entry informationi

Entry nameiCDK8_CAEBR
AccessioniPrimary (citable) accession number: P0C661
Secondary accession number(s): A8XFM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 24, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3