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Protein

RAS guanyl-releasing protein 2

Gene

Rasgrp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi439 – 450121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi468 – 479122PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri498 – 54851Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RAS guanyl-releasing protein 2
Alternative name(s):
Calcium and DAG-regulated guanine nucleotide exchange factor I
Short name:
CalDAG-GEFI
Gene namesi
Name:Rasgrp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1311630. Rasgrp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608RAS guanyl-releasing protein 2PRO_0000315610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161PhosphoserineBy similarity
Modified residuei117 – 1171PhosphoserineBy similarity
Modified residuei147 – 1471PhosphoserineBy similarity
Modified residuei554 – 5541PhosphoserineBy similarity
Modified residuei575 – 5751PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0C643.

PTM databases

iPTMnetiP0C643.
PhosphoSiteiP0C643.
SwissPalmiP0C643.

Expressioni

Tissue specificityi

Expressed in striatal neurons (at protein level). Expressed in the hematopoietic system. Detected in olfactory structures and deep cortical layers of brain.2 Publications

Interactioni

Subunit structurei

Forms a signaling complex with RAP1 and BRAF. Interacts with F-actin (By similarity). Interacts with RAP1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028646.

Structurei

3D structure databases

ProteinModelPortaliP0C643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 126123N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini154 – 387234Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini426 – 46136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini455 – 49036EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminal Ras-GEF domain mediates association with F-actin.By similarity

Sequence similaritiesi

Belongs to the RASGRP family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri498 – 54851Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3417. Eukaryota.
ENOG410XR96. LUCA.
HOGENOMiHOG000293171.
HOVERGENiHBG007513.
InParanoidiP0C643.
KOiK12361.
PhylomeDBiP0C643.
TreeFamiTF312918.

Family and domain databases

CDDicd06224. REM. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.840.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF13202. EF-hand_5. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00054. EFh. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF48366. SSF48366. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C643-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTLDLDKG CTVEELLRGC IEAFDDSGKV RDPQLVRMFL MMHPWYIPSS
60 70 80 90 100
QLASKLLHFY QQSRKDNSNS LQVKTCHLVR YWVSAFPAEF DLNPELAEQI
110 120 130 140 150
KELKALLDQE GNRRHSSLID IESVPTYKWK RQVTQRNPVE QKKRKMSLLF
160 170 180 190 200
DHLEPMELAE HLTYLEYRSF CKILFQDYHS FVTHGCTVDN PVLERFISLF
210 220 230 240 250
NSVSQWVQLM ILSKPTATQR ALVITHFVHV AEKLLQLQNF NTLMAVVGGL
260 270 280 290 300
SHSSISRLKE THSHVSPDTI KLWEGLTELV TATGNYSNYR RRLAACVGFR
310 320 330 340 350
FPILGVHLKD LVALQLALPD WLDPGRTRLN GAKMRQLFSI LEELAMVTSL
360 370 380 390 400
RPPVQANPDL LSLLTVSLDQ YQTEDELYQL SLQREPRSKS SPTSPTSCTP
410 420 430 440 450
PPRPPVLEEW TSVAKPKLDQ ALVAEHIEKM VESVFRNFDV DGDGHISQEE
460 470 480 490 500
FQIIRGNFPY LSAFGDLDQN QDGCISREEM ISYFLRSSSV LGGRMGFVHN
510 520 530 540 550
LQESNSLRPV ACRHCKALIL GIYKQGLKCR ACGVNCHKQC KDRLSVECRR
560 570 580 590 600
RAQSVSLEGS APSPSPTHTH HRAFSFSLPR PGRRSSRPPE IREEEVQTVE

DGVFDIHL
Length:608
Mass (Da):69,291
Last modified:January 15, 2008 - v1
Checksum:i0AA7ACFCEE989112
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03006336 Genomic DNA. No translation available.
AABR03008223 Genomic DNA. No translation available.
AABR03009977 Genomic DNA. No translation available.
AABR03011305 Genomic DNA. No translation available.
RefSeqiNP_001076446.1. NM_001082977.1.
UniGeneiRn.38473.

Genome annotation databases

GeneIDi361714.
KEGGirno:361714.
UCSCiRGD:1311630. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03006336 Genomic DNA. No translation available.
AABR03008223 Genomic DNA. No translation available.
AABR03009977 Genomic DNA. No translation available.
AABR03011305 Genomic DNA. No translation available.
RefSeqiNP_001076446.1. NM_001082977.1.
UniGeneiRn.38473.

3D structure databases

ProteinModelPortaliP0C643.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028646.

PTM databases

iPTMnetiP0C643.
PhosphoSiteiP0C643.
SwissPalmiP0C643.

Proteomic databases

PaxDbiP0C643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi361714.
KEGGirno:361714.
UCSCiRGD:1311630. rat.

Organism-specific databases

CTDi10235.
RGDi1311630. Rasgrp2.

Phylogenomic databases

eggNOGiKOG3417. Eukaryota.
ENOG410XR96. LUCA.
HOGENOMiHOG000293171.
HOVERGENiHBG007513.
InParanoidiP0C643.
KOiK12361.
PhylomeDBiP0C643.
TreeFamiTF312918.

Miscellaneous databases

PROiP0C643.

Family and domain databases

CDDicd06224. REM. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.840.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF13202. EF-hand_5. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00054. EFh. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF48366. SSF48366. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRP2_RAT
AccessioniPrimary (citable) accession number: P0C643
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.