Reviewed, UniProtKB/Swiss-Prot P0C622 (CMTB_PSEPU)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase
    EC=1.3.1.58
Alternative name(s):
    Biphenyl-2,3-dihydro-2,3-diol dehydrogenase

Gene names

Name:

cmtB

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cis-5,6-dihydroxy-4-isopropylcyclohexa-1,3-dienecarboxylate + NAD+ = 2,3-dihydroxy-p-cumate + NADH.
Pathway	Aromatic compound metabolism; p-cumate degradation; acetaldehyde and pyruvate from p-cumate: step 2/7.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase		PRO_0000054550
Regions
	Nucleotide binding	18 – 42	25	NAD By similarity
Sites
	Active site	163	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C622-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: B4CD9C3DDC833A8B	FASTA	259	27,310
	10 20 30 40 50 60 MSTLSRNALP LEGQVAVVTG GAHGIGLGIV ERLLGLGARV TASDIDESGL SLLCERLAAK 70 80 90 100 110 120 HADAIAVHAA DLSEEQGAQG LHRAAVERFG SVQILVNCAG GGVIRPFLEH TPETLKATID 130 140 150 160 170 180 RNLWTALWCS RVFLPDMLAR QYGRIINIGA DSVRNGLPDH AAYNAAKGGM HGLTTGLARE 190 200 210 220 230 240 FARQGVTVNT VAPCAVNTEV WVRIKNANPE LAQRFLDVIP MGRVGEIEEV ASMVGYLAQP 250 EAAFVTGQVI SVNGGSTML

References

[1]

"Pseudomonas putida CE2010 can degrade biphenyl by a mosaic pathway encoded by the tod operon and cmtE, which are identical to those of P. putida F1 except for a single base difference in the operator-promoter region of the cmt operon." Ohta Y., Maeda M., Kudo T. Microbiology 147:31-41(2001) [PubMed: 11160798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CE2010.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB042508 Genomic DNA. Translation: BAB17774.1.
3D structure databases
HSSP	HSSP built from PDB template 2AE1 based on UniProtKB P50163.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.58. 403.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMTB_PSEPU

Accession

Primary (citable) accession number: P0C622
Secondary accession number(s): Q51977

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	October 13, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)