P0C621 (TODA_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component
EC=1.18.1.3

Gene names

Name:

todA

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the electron transfer component of toluene 1,2-dioxygenase, transfers electrons from ferredoxin (todB) to NADH.
Catalytic activity	Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.
Cofactor	FAD.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (todC1 and todC2), a ferredoxin (todB) and a ferredoxin reductase (todA).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ferredoxin-NAD+ reductase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 409	408	Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component		PRO_0000167654
Regions
	Nucleotide binding	4 – 35	32	FAD Potential
	Nucleotide binding	145 – 173	29	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P0C621 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 20451B8A657FB895	FASTA	409	42,820
	10 20 30 40 50 60 MATHVAIIGN GVGGFTTAQA LRAEGFEGRI SLIGDEPHLP YDRPSLSKAV LDGSLERPPI 70 80 90 100 110 120 LAEADWYGEA RIDMLTGPEV TALDVQTRTI SLDDGTTLSA DAIVIATGSR ARTMALPGSQ 130 140 150 160 170 180 LPGVVTLRTY GDVQVLRDSW TSATRLLIVG GGLIGCEVAT TARKLGLSVT ILEAGDELLV 190 200 210 220 230 240 RVLGRRIGAW LRGLLTELGV QVELGTGVVG FSGEGQLEQV MASDGRSFVA DSALICVGAE 250 260 270 280 290 300 PADQLARQAG LACDRGVIVD HCGATLAKGY SPSEMWPVGA AAGGRRSLET YMNAQRQAAA 310 320 330 340 350 360 VAAAILGKNV SAPQLPVSWT EIAGHRMQMA GDIEGPGDFV SRGMPGSGAA LLFRLQERRI 370 380 390 400 QAVVAVDAPR DFALATRLVE ARAAIEPARL ADLSNSMRDF VRANEGDLT

References

[1]	"Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida." Irie S., Doi S., Yorifuji T., Takagi M., Yano K. J. Bacteriol. 169:5174-5179(1987) [PubMed: 3667527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BE-81.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17904 Genomic DNA. No translation available.
PIR	D36516.
3D structure databases
ProteinModelPortal	P0C621.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TODA_PSEPU

Accession

Primary (citable) accession number: P0C621
Secondary accession number(s): P13452

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	September 21, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program