ID   BNZB_PSEPU              Reviewed;         187 AA.
AC   P0C619; P08085; P13451;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-MAR-2009, entry version 7.
DE   RecName: Full=Benzene 1,2-dioxygenase subunit beta;
DE            EC=1.14.12.3;
DE   AltName: Full=Benzene 1,2-dioxygenase P2 subunit;
DE   AltName: Full=Toluene 2,3-dioxygenase subunit beta;
DE            EC=1.14.12.11;
GN   Name=bnzB; Synonyms=todC2;
OS   Pseudomonas putida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BE-81;
RX   MEDLINE=88032840; PubMed=3667527;
RA   Irie S., Doi S., Yorifuji T., Takagi M., Yano K.;
RT   "Nucleotide sequencing and characterization of the genes encoding
RT   benzene oxidation enzymes of Pseudomonas putida.";
RL   J. Bacteriol. 169:5174-5179(1987).
CC   -!- FUNCTION: Catalyzes both the oxidation of benzene and toluene. The
CC       beta subunit may be responsible for the substrate specificity of
CC       the enzyme.
CC   -!- CATALYTIC ACTIVITY: Benzene + NADH + O(2) = cis-cyclohexa-3,5-
CC       diene-1,2-diol + NAD(+).
CC   -!- CATALYTIC ACTIVITY: Toluene + NADH + O(2) = (1S,2R)-3-
CC       methylcyclohexa-3,5-diene-1,2-diol + NAD(+).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation;
CC       catechol from benzene: step 1/2.
CC   -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC   -!- PATHWAY: Xenobiotic degradation; xylene degradation.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (bnzA and bnzB), a
CC       ferredoxin (bnzC) and a ferredoxin reductase (bnzD).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; M17904; AAA25736.1; -; Genomic_DNA.
DR   PIR; B29830; B29830.
DR   BRENDA; 1.14.12.11; 403.
DR   BRENDA; 1.14.12.3; 403.
DR   GO; GO:0018619; F:benzene 1,2-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0018624; F:toluene dioxygenase activity; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; NAD; Oxidoreductase.
FT   CHAIN         1    187       Benzene 1,2-dioxygenase subunit beta.
FT                                /FTId=PRO_0000085067.
SQ   SEQUENCE   187 AA;  22013 MW;  39A5D2BECB6116B5 CRC64;
     MIDSANRADV FLRKPAPVAP ELQHEVEQFY YWEAKLLNDR RFEEWFALLA EDIHYFMPIR
     TTRIMRDSRL EYSGSREYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HLVSNVMIVG
     AEAEGEYEIS SAFIVYRNRL ERQLDIFAGE RRDTLRRNTS EAGFEIVNRT ILIDQSTILA
     NNLSFFF
//