Reviewed, UniProtKB/Swiss-Prot P0C619 (BNZB_PSEPU)

Names and origin

Protein names

Recommended name:
    Benzene 1,2-dioxygenase subunit beta
    EC=1.14.12.3
Alternative name(s):
    Benzene 1,2-dioxygenase P2 subunit
    Toluene 2,3-dioxygenase subunit beta
    EC=1.14.12.11

Gene names

Name:	bnzB
Synonyms:	todC2

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes both the oxidation of benzene and toluene. The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Benzene + NADH + O(2) = cis-cyclohexa-3,5-diene-1,2-diol + NAD(+). Toluene + NADH + O(2) = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD(+).
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 1 iron ion per subunit By similarity.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 1/2. Xenobiotic degradation; toluene degradation. Xenobiotic degradation; xylene degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bnzA and bnzB), a ferredoxin (bnzC) and a ferredoxin reductase (bnzD).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	benzene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW toluene dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Benzene 1,2-dioxygenase subunit beta		PRO_0000085067

Sequences

	Sequence		Length	Mass (Da)
	P0C619-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 39A5D2BECB6116B5	FASTA	187	22,013
	10 20 30 40 50 60 MIDSANRADV FLRKPAPVAP ELQHEVEQFY YWEAKLLNDR RFEEWFALLA EDIHYFMPIR 70 80 90 100 110 120 TTRIMRDSRL EYSGSREYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HLVSNVMIVG 130 140 150 160 170 180 AEAEGEYEIS SAFIVYRNRL ERQLDIFAGE RRDTLRRNTS EAGFEIVNRT ILIDQSTILA NNLSFFF

References

[1]	"Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida." Irie S., Doi S., Yorifuji T., Takagi M., Yano K. J. Bacteriol. 169:5174-5179(1987) [PubMed: 3667527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BE-81.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17904 Genomic DNA. Translation: AAA25736.1.
PIR	B29830.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000391. Rng_hydr_dOase-B. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BNZB_PSEPU

Accession

Primary (citable) accession number: P0C619
Secondary accession number(s): P08085, P13451

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	November 4, 2008
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)