P0C5I2 (GMPPB_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-1-phosphate guanyltransferase beta
EC=2.7.7.13

Alternative name(s):
GDP-mannose pyrophosphorylase B
GTP-mannose-1-phosphate guanylyltransferase beta

Gene names

Name:

GMPPB

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Enzyme regulation	Activated by Mn2+. Ref.1
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.
Biophysicochemical properties	Kinetic parameters: KM=0.2 mM for mannose-1-phosphate Ref.1 KM=0.36 mM for GTP KM=39 nM for GDP-mannose Vmax=0.56 mmol/min/mg enzyme toward mannose-1-phosphate Vmax=14 nmol/min/mg enzyme toward GDP-mannose

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Mannose-1-phosphate guanyltransferase beta		PRO_0000307164

Sequences

	Sequence		Length	Mass (Da)
	P0C5I2 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 723579381B292BB4	FASTA	360	39,827
	10 20 30 40 50 60 MKALILVGGY GTRLRPLTLS IPKPLVDFCN KPILLHQVEA LASAGVDHVI LAVSYMSQML 70 80 90 100 110 120 EKEMKAQEQR LGIRISMSHE EEPLGTAGPL ALARDLLSET AEPFFVLNSD VICDFPFQAM 130 140 150 160 170 180 VQFHRHHGQE GSILVTKVEE PSKYGVVVCE ADTGRIHRFV EKPQVFVSNK INAGMYILSP 190 200 210 220 230 240 AVLQRIQLQP TSIEKEIFPV MAKEGQLYAM ELQGFWMDIG QPKDFLTGMC LFLQSLRQKQ 250 260 270 280 290 300 PEQLCSGPGI VGNVLVDPSA RIGKNCSIGP NVSLGPGVVV EDGVCIRRCT VLRDARIRSH 310 320 330 340 350 360 SWLESCIVCW RCRVGQWVRM ENVTVLGEDV IVNDELYLNG ASVLPHKSIG ESVPEPGIIM

References

[1]

"Cloning, expression and characterization of the pig liver GDP-mannose pyrophosphorylase. Evidence that GDP-mannose and GDP-Glc pyrophosphorylases are different proteins." Ning B., Elbein A.D. Eur. J. Biochem. 267:6866-6874(2000) [PubMed: 11082198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-23; 159-170 AND 180-194, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

3D structure databases
ProteinModelPortal	P0C5I2.
ModBase	Search...
Protein-protein interaction databases
STRING	P0C5I2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG107955.
OrthoDB	EOG48D0VN.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00132. Hexapep. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMPPB_PIG

Accession

Primary (citable) accession number: P0C5I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	September 21, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program