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Protein

Gibberellin 20 oxidase 2

Gene

20ox2

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA53 to GA20 via a three-step oxidation at C-20 of the GA skeleton.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi249IronPROSITE-ProRule annotation1
Metal bindingi251IronPROSITE-ProRule annotation1
Metal bindingi305IronPROSITE-ProRule annotation1
Active sitei315Sequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 20 oxidase 2 (EC:1.14.11.-)
Alternative name(s):
GA 20-oxidase 2
Gibberellin C-20 oxidase 2
Os20ox2
Protein semidwarf-1
Gene namesi
Name:20ox2
Synonyms:C20ox2, GA20, Sd-1
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza

Pathology & Biotechi

Biotechnological usei

This gene, also known as the 'green revolution gene' has been introduced by conventional breeding procedures into several cultivars. It provides rice cultivars with short, thick culms, raises the harvest index, improves lodging resistance and responsiveness to nitrogen fertilizer, resulting high yields without affecting panicle and grain quality.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003060901 – 389Gibberellin 20 oxidase 2Add BLAST389

Structurei

3D structure databases

ProteinModelPortaliP0C5H5.
SMRiP0C5H5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 324Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000276735.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiView protein in InterPro
IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C5H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAEHPTPPQ PHQPPPMDST AGSGIAAPAA AAVCDLRMEP KIPEPFVWPN
60 70 80 90 100
GDARPASAAE LDMPVVDVGV LRDGDAEGLR RAAAQVAAAC ATHGFFQVSE
110 120 130 140 150
HGVDAALARA ALDGASDFFR LPLAEKRRAR RVPGTVSGYT SAHADRFASK
160 170 180 190 200
LPWKETLSFG FHDRAAAPVV ADYFSSTLGP DFAPMGRVYQ KYCEEMKELS
210 220 230 240 250
LTIMELLELS LGVERGYYRE FFADSSSIMR CNYYPPCPEP ERTLGTGPHC
260 270 280 290 300
DPTALTILLQ DDVGGLEVLV DGEWRPVSPV PGAMVINIGD TFMALSNGRY
310 320 330 340 350
KSCLHRAVVN QRRERRSLAF FLCPREDRVV RPPPSAATPQ HYPDFTWADL
360 370 380
MRFTQRHYRA DTRTLDAFTR WLAPPAADAA ATAQVEAAS
Length:389
Mass (Da):42,513
Last modified:October 2, 2007 - v1
Checksum:i01B26E496DF2EAC1
GO

Polymorphismi

Sd-1 is the major semidwarfing allele extensively used in modern rice cultivars.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti100 – 102EHG → ARR in strain: cv. Doongara, cv. Habataki, cv. IR24 and cv. Milyang 23; allele sd-1; semidwarfing. 3
Natural varianti103 – 389Missing in strain: cv. Doongara, cv. Habataki, cv. IR24 and cv. Milyang 23; allele sd-1; semidwarfing. 2 PublicationsAdd BLAST287

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY114310 mRNA. Translation: AAM56041.1.
AF465256 Genomic DNA. Translation: AAL87950.1.
AY115558 Genomic DNA. Translation: AAN73383.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY114310 mRNA. Translation: AAM56041.1.
AF465256 Genomic DNA. Translation: AAL87950.1.
AY115558 Genomic DNA. Translation: AAN73383.1.

3D structure databases

ProteinModelPortaliP0C5H5.
SMRiP0C5H5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000276735.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiView protein in InterPro
IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGAOX2_ORYSI
AccessioniPrimary (citable) accession number: P0C5H5
Secondary accession number(s): Q6YLX3
, Q7EC22, Q7GBH4, Q8RVF5, Q8S492
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: February 15, 2017
This is version 40 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A second gene, 20ox1, is preferentially expressed in the reproductive organs and enables the flowers in sd1 plants to develop and be fertilized normally.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.