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P0C5D3 (PTPB_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Low molecular weight protein-tyrosine-phosphatase PtpB

EC=3.1.3.48
Alternative name(s):
Phosphotyrosine phosphatase B
Short name=PTPase B
Gene names
Name:ptpB
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length139 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1

Enzyme regulation

Inhibited by N-ethylmaleimide and sodium orthovanadate. Ref.1

Sequence similarities

Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Biophysicochemical properties

Kinetic parameters:

KM=1.5 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius) Ref.1

Vmax=1.4 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)

pH dependence:

Optimum pH is 5.7-6.5.

Temperature dependence:

Optimum temperature is about 40 degrees Celsius.

Ontologies

Keywords
   Molecular functionHydrolase
Protein phosphatase
Gene Ontology (GO)
   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 139139Low molecular weight protein-tyrosine-phosphatase PtpB
PRO_0000300670

Sites

Active site71Nucleophile By similarity
Active site131 By similarity
Active site1111Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C5D3 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 63EB2C0E2A53EA5B

FASTA13915,788
        10         20         30         40         50         60 
MKILFVCTGN TCRSPLAESI AKEVMPNHQF ESRGIFAVNN QGVSNYVEDL VEEHHLAETT 

        70         80         90        100        110        120 
LSQQFTEADL KADIILTMSY SHKELIEAHF GLQNHVFTLH EYVKEAGEVI DPYGGTKEMY 

       130 
VHTYEELVSL ILKLKDIIC 

« Hide

References

[1]"Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: Reynolds.

Cross-references

3D structure databases

ProteinModelPortalP0C5D3.
SMRP0C5D3. Positions 1-134.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

PptaseDBP3D0411153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
[Graphical view]
PfamPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSPR00719. LMWPTPASE.
SMARTSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMSSF52788. SSF52788. 1 hit.
ProtoNetSearch...

Entry information

Entry namePTPB_STAAU
AccessionPrimary (citable) accession number: P0C5D3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families