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P0C5D3

- PTPB_STAAU

UniProt

P0C5D3 - PTPB_STAAU

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Protein
Low molecular weight protein-tyrosine-phosphatase PtpB
Gene
ptpB
Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

Kineticsi

  1. KM=1.5 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication

Vmax=1.4 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)

pH dependencei

Optimum pH is 5.7-6.5.

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei7 – 71Nucleophile By similarity
Active sitei13 – 131 By similarity
Active sitei111 – 1111Proton donor By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Protein family/group databases

PptaseDBiP3D0411153.

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpB (EC:3.1.3.48)
Alternative name(s):
Phosphotyrosine phosphatase B
Short name:
PTPase B
Gene namesi
Name:ptpB
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139Low molecular weight protein-tyrosine-phosphatase PtpB
PRO_0000300670Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP0C5D3.
SMRiP0C5D3. Positions 1-134.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717. PTHR11717. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C5D3-1 [UniParc]FASTAAdd to Basket

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MKILFVCTGN TCRSPLAESI AKEVMPNHQF ESRGIFAVNN QGVSNYVEDL    50
VEEHHLAETT LSQQFTEADL KADIILTMSY SHKELIEAHF GLQNHVFTLH 100
EYVKEAGEVI DPYGGTKEMY VHTYEELVSL ILKLKDIIC 139
Length:139
Mass (Da):15,788
Last modified:September 11, 2007 - v1
Checksum:i63EB2C0E2A53EA5B
GO

Cross-referencesi

3D structure databases

ProteinModelPortali P0C5D3.
SMRi P0C5D3. Positions 1-134.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

PptaseDBi P3D0411153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view ]
PANTHERi PTHR11717. PTHR11717. 1 hit.
Pfami PF01451. LMWPc. 1 hit.
[Graphical view ]
PRINTSi PR00719. LMWPTPASE.
SMARTi SM00226. LMWPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52788. SSF52788. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
    Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
    J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: Reynolds.

Entry informationi

Entry nameiPTPB_STAAU
AccessioniPrimary (citable) accession number: P0C5D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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