Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0C5D3

- PTPB_STAAU

UniProt

P0C5D3 - PTPB_STAAU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Low molecular weight protein-tyrosine-phosphatase PtpB

Gene

ptpB

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

Kineticsi

  1. KM=1.5 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication

Vmax=1.4 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 5.7-6.5.1 Publication

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei7 – 71NucleophileBy similarity
Active sitei13 – 131By similarity
Active sitei111 – 1111Proton donorBy similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Protein family/group databases

PptaseDBiP3D0411153.

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpB (EC:3.1.3.48)
Alternative name(s):
Phosphotyrosine phosphatase B
Short name:
PTPase B
Gene namesi
Name:ptpB
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139Low molecular weight protein-tyrosine-phosphatase PtpBPRO_0000300670Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP0C5D3.
SMRiP0C5D3. Positions 1-134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF14. PTHR11717:SF14. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C5D3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKILFVCTGN TCRSPLAESI AKEVMPNHQF ESRGIFAVNN QGVSNYVEDL
60 70 80 90 100
VEEHHLAETT LSQQFTEADL KADIILTMSY SHKELIEAHF GLQNHVFTLH
110 120 130
EYVKEAGEVI DPYGGTKEMY VHTYEELVSL ILKLKDIIC
Length:139
Mass (Da):15,788
Last modified:September 11, 2007 - v1
Checksum:i63EB2C0E2A53EA5B
GO

Sequence databases

RefSeqiWP_000697334.1. NZ_JFFV01000012.1.

Cross-referencesi

Sequence databases

RefSeqi WP_000697334.1. NZ_JFFV01000012.1.

3D structure databases

ProteinModelPortali P0C5D3.
SMRi P0C5D3. Positions 1-134.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

PptaseDBi P3D0411153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view ]
PANTHERi PTHR11717:SF14. PTHR11717:SF14. 1 hit.
Pfami PF01451. LMWPc. 1 hit.
[Graphical view ]
PRINTSi PR00719. LMWPTPASE.
SMARTi SM00226. LMWPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52788. SSF52788. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
    Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
    J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: Reynolds.

Entry informationi

Entry nameiPTPB_STAAU
AccessioniPrimary (citable) accession number: P0C5D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3