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Protein

Low molecular weight protein-tyrosine-phosphatase PtpB

Gene

ptpB

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

Kineticsi

  1. KM=1.5 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication
  1. Vmax=1.4 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 5.7-6.5.1 Publication

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei7NucleophileBy similarity1
Active sitei13By similarity1
Active sitei111Proton donorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpB (EC:3.1.3.48)
Alternative name(s):
Phosphotyrosine phosphatase B
Short name:
PTPase B
Gene namesi
Name:ptpB
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003006701 – 139Low molecular weight protein-tyrosine-phosphatase PtpBAdd BLAST139

Structurei

3D structure databases

ProteinModelPortaliP0C5D3.
SMRiP0C5D3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107WAU. Bacteria.
COG0394. LUCA.

Family and domain databases

CDDicd00115. LMWPc. 1 hit.
InterProiView protein in InterPro
IPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
PfamiView protein in Pfam
PF01451. LMWPc. 1 hit.
PRINTSiPR00719. LMWPTPASE.
SMARTiView protein in SMART
SM00226. LMWPc. 1 hit.
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C5D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILFVCTGN TCRSPLAESI AKEVMPNHQF ESRGIFAVNN QGVSNYVEDL
60 70 80 90 100
VEEHHLAETT LSQQFTEADL KADIILTMSY SHKELIEAHF GLQNHVFTLH
110 120 130
EYVKEAGEVI DPYGGTKEMY VHTYEELVSL ILKLKDIIC
Length:139
Mass (Da):15,788
Last modified:September 11, 2007 - v1
Checksum:i63EB2C0E2A53EA5B
GO

Sequence databases

RefSeqiWP_000697334.1. NZ_NBZY01000022.1.

Genome annotation databases

GeneIDi31214848.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPTPB_STAAU
AccessioniPrimary (citable) accession number: P0C5D3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: July 5, 2017
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families