P0C5D3 (PTPB_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpB
EC=3.1.3.48

Alternative name(s):
Phosphotyrosine phosphatase B
Short name=PTPase B

Gene names

Name:

ptpB

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dephosphorylates the phosphotyrosine-containing proteins.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1
Enzyme regulation	Inhibited by N-ethylmaleimide and sodium orthovanadate. Ref.1
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
Biophysicochemical properties	Kinetic parameters: KM=1.5 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius) Ref.1 Vmax=1.4 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius) pH dependence: Optimum pH is 5.7-6.5. Temperature dependence: Optimum temperature is about 40 degrees Celsius.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 139	139	Low molecular weight protein-tyrosine-phosphatase PtpB		PRO_0000300670
Sites
	Active site	7	1	Nucleophile By similarity
	Active site	13	1	By similarity
	Active site	111	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C5D3 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 63EB2C0E2A53EA5B	FASTA	139	15,788
	10 20 30 40 50 60 MKILFVCTGN TCRSPLAESI AKEVMPNHQF ESRGIFAVNN QGVSNYVEDL VEEHHLAETT 70 80 90 100 110 120 LSQQFTEADL KADIILTMSY SHKELIEAHF GLQNHVFTLH EYVKEAGEVI DPYGGTKEMY 130 VHTYEELVSL ILKLKDIIC

References

[1]

"Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases." Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J. J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: Reynolds.

Cross-references

3D structure databases
ProteinModelPortal	P0C5D3.
SMR	P0C5D3. Positions 1-134.
ModBase	Search...
Protein family/group databases
PptaseDB	P3D0411153.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR023485. Ptyr_pPase_SF. IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. PTHR11717. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
SUPFAM	SSF52788. Tyr_Pase_low_mol_wt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTPB_STAAU

Accession

Primary (citable) accession number: P0C5D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program