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P0C5D3

- PTPB_STAAU

UniProt

P0C5D3 - PTPB_STAAU

Protein

Low molecular weight protein-tyrosine-phosphatase PtpB

Gene

ptpB

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Dephosphorylates the phosphotyrosine-containing proteins.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

    Enzyme regulationi

    Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

    Kineticsi

    1. KM=1.5 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication

    Vmax=1.4 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 5.7-6.5.1 Publication

    Temperature dependencei

    Optimum temperature is about 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei7 – 71NucleophileBy similarity
    Active sitei13 – 131By similarity
    Active sitei111 – 1111Proton donorBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Protein family/group databases

    PptaseDBiP3D0411153.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight protein-tyrosine-phosphatase PtpB (EC:3.1.3.48)
    Alternative name(s):
    Phosphotyrosine phosphatase B
    Short name:
    PTPase B
    Gene namesi
    Name:ptpB
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 139139Low molecular weight protein-tyrosine-phosphatase PtpBPRO_0000300670Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0C5D3.
    SMRiP0C5D3. Positions 1-134.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0C5D3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILFVCTGN TCRSPLAESI AKEVMPNHQF ESRGIFAVNN QGVSNYVEDL    50
    VEEHHLAETT LSQQFTEADL KADIILTMSY SHKELIEAHF GLQNHVFTLH 100
    EYVKEAGEVI DPYGGTKEMY VHTYEELVSL ILKLKDIIC 139
    Length:139
    Mass (Da):15,788
    Last modified:September 11, 2007 - v1
    Checksum:i63EB2C0E2A53EA5B
    GO

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P0C5D3.
    SMRi P0C5D3. Positions 1-134.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    PptaseDBi P3D0411153.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
      Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
      J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: Reynolds.

    Entry informationi

    Entry nameiPTPB_STAAU
    AccessioniPrimary (citable) accession number: P0C5D3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3