P0C5D2 (PTPA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpA
EC=3.1.3.48

Alternative name(s):
Phosphotyrosine phosphatase A
Short name=PTPase A

Gene names

Name:

ptpA

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dephosphorylates the phosphotyrosine-containing proteins.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1
Enzyme regulation	Inhibited by N-ethylmaleimide and sodium orthovanadate. Ref.1
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
Biophysicochemical properties	Kinetic parameters: KM=1.2 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius) Ref.1 Vmax=33.6 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius) pH dependence: Optimum pH is 6.2. Temperature dependence: Optimum temperature is about 40 degrees Celsius.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 154

154

Low molecular weight protein-tyrosine-phosphatase PtpA

PRO_0000300656

Sites

Active site

8

1

Nucleophile By similarity

Active site

14

1

By similarity

Active site

120

1

Proton donor By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

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.

.

.

.

.

.

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154

Helix Strand Turn

Details...

Beta strand

2 – 13

12

Helix

14 – 28

15

Beta strand

33 – 40

8

Helix

52 – 60

9

Beta strand

80 – 86

7

Helix

87 – 96

10

Beta strand

102 – 106

5

Helix

107 – 110

4

Helix

121 – 124

4

Helix

127 – 148

22

Sequences

	Sequence		Length	Mass (Da)
	P0C5D2 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 67E81E0B8125B1E8	FASTA	154	17,491
	10 20 30 40 50 60 MVDVAFVCLG NICRSPMAEA IMRQRLKDRN IHDIKVHSRG TGSWNLGEPP HEGTQKILNK 70 80 90 100 110 120 HNIPFDGMIS ELFEATDDFD YIVAMDQSNV DNIKSINPNL KGQLFKLLEF SNMEESDVPD 130 140 150 PYYTNNFEGV YDMVLSSCDN LIDYIVKDAN LKEG

References

[1]	"Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases." Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J. J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: Reynolds.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ROF	X-ray	1.03	A	1-152	[»]

ProteinModelPortal

P0C5D2.

SMR

P0C5D2. Positions 1-152.

ModBase

Search...

Protein family/group databases

PptaseDB

P3D0411152.

Proteomic databases

PRIDE

P0C5D2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]

PANTHER

PTHR11717. PTHR11717. 1 hit.

Pfam

PF01451. LMWPc. 1 hit.
[Graphical view]

PRINTS

PR00719. LMWPTPASE.

SMART

SM00226. LMWPc. 1 hit.
[Graphical view]

SUPFAM

SSF52788. Tyr_Pase_low_mol_wt. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PTPA_STAAU

Accession

Primary (citable) accession number: P0C5D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	January 9, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program