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Protein

Low molecular weight protein-tyrosine-phosphatase PtpA

Gene

ptpA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

Kineticsi

  1. KM=1.2 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication

Vmax=33.6 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 6.2.1 Publication

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81NucleophileBy similarity
Active sitei14 – 141By similarity
Active sitei120 – 1201Proton donorBy similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Protein family/group databases

PptaseDBiP3D0411152.

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpA (EC:3.1.3.48)
Alternative name(s):
Phosphotyrosine phosphatase A
Short name:
PTPase A
Gene namesi
Name:ptpA
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Low molecular weight protein-tyrosine-phosphatase PtpAPRO_0000300656Add
BLAST

Proteomic databases

PRIDEiP0C5D2.

Structurei

Secondary structure

1
154
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1312Combined sources
Helixi14 – 2815Combined sources
Beta strandi33 – 408Combined sources
Helixi52 – 609Combined sources
Beta strandi80 – 867Combined sources
Helixi87 – 9610Combined sources
Beta strandi102 – 1065Combined sources
Helixi107 – 1104Combined sources
Helixi121 – 1244Combined sources
Helixi127 – 14822Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ROFX-ray1.03A1-152[»]
ProteinModelPortaliP0C5D2.
SMRiP0C5D2. Positions 1-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C5D2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVDVAFVCLG NICRSPMAEA IMRQRLKDRN IHDIKVHSRG TGSWNLGEPP
60 70 80 90 100
HEGTQKILNK HNIPFDGMIS ELFEATDDFD YIVAMDQSNV DNIKSINPNL
110 120 130 140 150
KGQLFKLLEF SNMEESDVPD PYYTNNFEGV YDMVLSSCDN LIDYIVKDAN

LKEG
Length:154
Mass (Da):17,491
Last modified:September 11, 2007 - v1
Checksum:i67E81E0B8125B1E8
GO

Sequence databases

RefSeqiWP_000228666.1. NZ_JQIZ01000036.1.

Cross-referencesi

Sequence databases

RefSeqiWP_000228666.1. NZ_JQIZ01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ROFX-ray1.03A1-152[»]
ProteinModelPortaliP0C5D2.
SMRiP0C5D2. Positions 1-152.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PptaseDBiP3D0411152.

Proteomic databases

PRIDEiP0C5D2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
    Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
    J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: Reynolds.

Entry informationi

Entry nameiPTPA_STAAU
AccessioniPrimary (citable) accession number: P0C5D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: January 7, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.