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Protein

Low molecular weight protein-tyrosine-phosphatase PtpA

Gene

ptpA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

Kineticsi

  1. KM=1.2 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication
  1. Vmax=33.6 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 6.2.1 Publication

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8NucleophileBy similarity1
Active sitei14By similarity1
Active sitei120Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight protein-tyrosine-phosphatase PtpA (EC:3.1.3.48)
Alternative name(s):
Phosphotyrosine phosphatase A
Short name:
PTPase A
Gene namesi
Name:ptpA
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003006561 – 154Low molecular weight protein-tyrosine-phosphatase PtpAAdd BLAST154

Interactioni

Protein-protein interaction databases

STRINGi93062.SACOL1939.

Structurei

Secondary structure

1154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 13Combined sources12
Helixi14 – 28Combined sources15
Beta strandi33 – 40Combined sources8
Helixi52 – 60Combined sources9
Beta strandi80 – 86Combined sources7
Helixi87 – 96Combined sources10
Beta strandi102 – 106Combined sources5
Helixi107 – 110Combined sources4
Helixi121 – 124Combined sources4
Helixi127 – 148Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ROFX-ray1.03A1-152[»]
ProteinModelPortaliP0C5D2.
SMRiP0C5D2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107ZCW. Bacteria.
COG0394. LUCA.

Family and domain databases

CDDicd00115. LMWPc. 1 hit.
InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C5D2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDVAFVCLG NICRSPMAEA IMRQRLKDRN IHDIKVHSRG TGSWNLGEPP
60 70 80 90 100
HEGTQKILNK HNIPFDGMIS ELFEATDDFD YIVAMDQSNV DNIKSINPNL
110 120 130 140 150
KGQLFKLLEF SNMEESDVPD PYYTNNFEGV YDMVLSSCDN LIDYIVKDAN

LKEG
Length:154
Mass (Da):17,491
Last modified:September 11, 2007 - v1
Checksum:i67E81E0B8125B1E8
GO

Sequence databases

RefSeqiWP_000228666.1. NZ_MCFM01000005.1.

Genome annotation databases

GeneIDi28380476.

Cross-referencesi

Sequence databases

RefSeqiWP_000228666.1. NZ_MCFM01000005.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ROFX-ray1.03A1-152[»]
ProteinModelPortaliP0C5D2.
SMRiP0C5D2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL1939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi28380476.

Phylogenomic databases

eggNOGiENOG4107ZCW. Bacteria.
COG0394. LUCA.

Family and domain databases

CDDicd00115. LMWPc. 1 hit.
InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTPA_STAAU
AccessioniPrimary (citable) accession number: P0C5D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.