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P0C5D2

- PTPA_STAAU

UniProt

P0C5D2 - PTPA_STAAU

Protein

Low molecular weight protein-tyrosine-phosphatase PtpA

Gene

ptpA

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Dephosphorylates the phosphotyrosine-containing proteins.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

    Enzyme regulationi

    Inhibited by N-ethylmaleimide and sodium orthovanadate.1 Publication

    Kineticsi

    1. KM=1.2 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius)1 Publication

    Vmax=33.6 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.2.1 Publication

    Temperature dependencei

    Optimum temperature is about 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei8 – 81NucleophileBy similarity
    Active sitei14 – 141By similarity
    Active sitei120 – 1201Proton donorBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Protein family/group databases

    PptaseDBiP3D0411152.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight protein-tyrosine-phosphatase PtpA (EC:3.1.3.48)
    Alternative name(s):
    Phosphotyrosine phosphatase A
    Short name:
    PTPase A
    Gene namesi
    Name:ptpA
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 154154Low molecular weight protein-tyrosine-phosphatase PtpAPRO_0000300656Add
    BLAST

    Proteomic databases

    PRIDEiP0C5D2.

    Structurei

    Secondary structure

    1
    154
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312
    Helixi14 – 2815
    Beta strandi33 – 408
    Helixi52 – 609
    Beta strandi80 – 867
    Helixi87 – 9610
    Beta strandi102 – 1065
    Helixi107 – 1104
    Helixi121 – 1244
    Helixi127 – 14822

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ROFX-ray1.03A1-152[»]
    ProteinModelPortaliP0C5D2.
    SMRiP0C5D2. Positions 1-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0C5D2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVDVAFVCLG NICRSPMAEA IMRQRLKDRN IHDIKVHSRG TGSWNLGEPP    50
    HEGTQKILNK HNIPFDGMIS ELFEATDDFD YIVAMDQSNV DNIKSINPNL 100
    KGQLFKLLEF SNMEESDVPD PYYTNNFEGV YDMVLSSCDN LIDYIVKDAN 150
    LKEG 154
    Length:154
    Mass (Da):17,491
    Last modified:September 11, 2007 - v1
    Checksum:i67E81E0B8125B1E8
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ROF X-ray 1.03 A 1-152 [» ]
    ProteinModelPortali P0C5D2.
    SMRi P0C5D2. Positions 1-152.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    PptaseDBi P3D0411152.

    Proteomic databases

    PRIDEi P0C5D2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
      Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
      J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: Reynolds.

    Entry informationi

    Entry nameiPTPA_STAAU
    AccessioniPrimary (citable) accession number: P0C5D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3