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P0C5D2 (PTPA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Low molecular weight protein-tyrosine-phosphatase PtpA

EC=3.1.3.48
Alternative name(s):
Phosphotyrosine phosphatase A
Short name=PTPase A
Gene names
Name:ptpA
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length154 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dephosphorylates the phosphotyrosine-containing proteins.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1

Enzyme regulation

Inhibited by N-ethylmaleimide and sodium orthovanadate. Ref.1

Sequence similarities

Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Biophysicochemical properties

Kinetic parameters:

KM=1.2 mM for p-nitrophenyl-phosphate (at pH 6.2 and 37 degrees Celsius) Ref.1

Vmax=33.6 µmol/min/mg enzyme (at pH 6.2 and 37 degrees Celsius)

pH dependence:

Optimum pH is 6.2.

Temperature dependence:

Optimum temperature is about 40 degrees Celsius.

Ontologies

Keywords
   Molecular functionHydrolase
Protein phosphatase
   Technical term3D-structure
Gene Ontology (GO)
   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 154154Low molecular weight protein-tyrosine-phosphatase PtpA
PRO_0000300656

Sites

Active site81Nucleophile By similarity
Active site141 By similarity
Active site1201Proton donor By similarity

Secondary structure

.................. 154
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0C5D2 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 67E81E0B8125B1E8

FASTA15417,491
        10         20         30         40         50         60 
MVDVAFVCLG NICRSPMAEA IMRQRLKDRN IHDIKVHSRG TGSWNLGEPP HEGTQKILNK 

        70         80         90        100        110        120 
HNIPFDGMIS ELFEATDDFD YIVAMDQSNV DNIKSINPNL KGQLFKLLEF SNMEESDVPD 

       130        140        150 
PYYTNNFEGV YDMVLSSCDN LIDYIVKDAN LKEG 

« Hide

References

[1]"Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases."
Soulat D., Vaganay E., Duclos B., Genestier A.-L., Etienne J., Cozzone A.J.
J. Bacteriol. 184:5194-5199(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: Reynolds.
+Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ROFX-ray1.03A1-152[»]
ProteinModelPortalP0C5D2.
SMRP0C5D2. Positions 1-152.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

PptaseDBP3D0411152.

Proteomic databases

PRIDEP0C5D2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERPTHR11717. PTHR11717. 1 hit.
PfamPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSPR00719. LMWPTPASE.
SMARTSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMSSF52788. SSF52788. 1 hit.
ProtoNetSearch...

Entry information

Entry namePTPA_STAAU
AccessionPrimary (citable) accession number: P0C5D2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references