Reviewed, UniProtKB/Swiss-Prot P0C5B6 (4CLL4_ARATH)

Names and origin

Protein names

Recommended name:
    4-coumarate--CoA ligase-like 4
    EC=6.2.1.-

Gene names

Name:	4CLL4
Ordered Locus Names:	At1g20500
ORF Names:	F5M15.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Peroxisome Potential.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence AAF79612.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 3 genes: At1g20480, At1g20490 and At1g20500.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	4-coumarate--CoA ligase-like 4		PRO_0000299177
Regions
	Nucleotide binding	205 – 213	9	ATP By similarity
	Nucleotide binding	345 – 350	6	ATP By similarity
	Region	273 – 344	72	SBD1 By similarity
	Region	345 – 409	65	SBD2 By similarity
	Motif	548 – 550	3	Microbody targeting signal Potential
Sites
	Binding site	430	1	ATP By similarity
	Binding site	445	1	ATP By similarity
	Binding site	536	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C5B6-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 1ACD57613E6D1D81	FASTA	550	60,521
	10 20 30 40 50 60 MAYPERSLIV DPRSGFCKSN STFYSKRQPL SLPPNLSRDV TTFISSQPHR GKTAFIDAAT 70 80 90 100 110 120 GQCLTFSDLW RAVDRVADCL YHEVGIRRGD VVLILSPNSI FIPVVCLSVM SLGAVFTTAN 130 140 150 160 170 180 TLNTSGEISK QIADSNPTLV FTTRQLAPKL PVAISVVLTD DEVYQELTSA IRVVGILSEM 190 200 210 220 230 240 VKKEPSGQRV RDRVNQDDTA MMLYSSGTTG PSKGVISSHR NLTAHVARFI SDNLKRDDIF 250 260 270 280 290 300 ICTVPMFHTY GLLTFAMGTV ALGSTVVILR RFQLHDMMDA VEKHRATALA LAPPVLVAMI 310 320 330 340 350 360 NDADLIKAKY DLSSLKTVRC GGAPLSKEVT EGFLEKYPTV DILQGYALTE SNGGGAFTNS 370 380 390 400 410 420 AEESRRYGTA GTLTSDVEAR IVDPNTGRFM GINQTGELWL KGPSISKGYF KNQEATNETI 430 440 450 460 470 480 NLEGWLKTGD LCYIDEDGFL FVVDRLKELI KYKGYQVPPA ELEALLITHP DILDAAVIPF 490 500 510 520 530 540 PDKEAGQYPM AYVVRKHESN LSEKQVIDFI SKQVAPYKKI RKVSFINSIP KTASGKTLRK 550 DLIKLATSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases." Shockey J.M., Fulda M.S., Browse J. Plant Physiol. 132:1065-1076(2003) [PubMed: 12805634] [Abstract] Cited for: GENE FAMILY ORGANIZATION.
[3]	"The substrate specificity-determining amino acid code of 4-coumarate:CoA ligase." Schneider K., Hoevel K., Witzel K., Hamberger B., Schomburg D., Kombrink E., Stuible H.-P. Proc. Natl. Acad. Sci. U.S.A. 100:8601-8606(2003) [PubMed: 12819348] [Abstract] Cited for: GENE FAMILY ORGANIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC027665 Genomic DNA. Translation: AAF79612.1. Sequence problems.
IPI	IPI00529307.
PIR	D86338.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Proteomic databases
PRIDE	P0C5B6.
Genome annotation databases
GenomeReviews	Gene locus AT1G20500 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g20500.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CLL4_ARATH

Accession

Primary (citable) accession number: P0C5B6
Secondary accession number(s): Q9LMV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)