Reviewed, UniProtKB/Swiss-Prot P0C5A3 (DPNP_ORYSA)

Names and origin

Protein names	Recommended name:     3'(2'),5'-bisphosphate nucleotidase     EC=3.1.3.7 Alternative name(s):     3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase     DPNPase
Organism	Oryza sativa (Rice)
Taxonomic identifier	4530 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS.
Catalytic activity	Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by Ca2+, Li+, and Na+ and activated by K+.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	sulfur metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Inferred from electronic annotation. Source: EC inositol or phosphatidylinositol phosphatase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	3'(2'),5'-bisphosphate nucleotidase		PRO_0000296248
Sites
	Metal binding	78	1	Magnesium 1 By similarity
	Metal binding	140	1	Magnesium 1 By similarity
	Metal binding	140	1	Magnesium 2 By similarity
	Metal binding	142	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	143	1	Magnesium 2 By similarity
	Metal binding	292	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C5A3-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: EFE813C18BEDB5FD	FASTA	358	37,480
	10 20 30 40 50 60 MSQAAGNPYA AELAAAKKAV TLAARLCQAV QKDILQSGVQ SKADQSPVTV ADYGSQILVS 70 80 90 100 110 120 LVLKMEAPAS SSFSMVAEED SEELRKEGAE EILENITELV NETIVDDGTY SIYFSKEGIL 130 140 150 160 170 180 SAIDDGKSEG GPSGRHWVLD PIDGTKGFLR GDQYAIALAL LDEGKVVLGV LACPNLSLGS 190 200 210 220 230 240 IGNLNGGSSG DQVGALFSAT IGCGAEVESL QGSPAQKISV CSIDNPVEAS FFESYEGAHS 250 260 270 280 290 300 LRDLTGSIAE KLGVQAPPVR IDSQAKYGAL ARGDGAIYLR FPHKGYREKI WDHAAGSIVV 310 320 330 340 350 TEAGGLVTDA SGNDLDFSKG RFLDLDTGII ATNKQLMPSL LKAVQDAIKE QNQAASPL

References

[1]

"A rice HAL2-like gene encodes a Ca(2+)-sensitive 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase and complements yeast met22 and Escherichia coli cysQ mutations." Peng Z., Verma D.P.S. J. Biol. Chem. 270:29105-29110(1995) [PubMed: 7493934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Tallahamsa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U33283 mRNA. Translation: AAC49121.1.
PIR	T03305.
3D structure databases
HSSP	HSSP built from PDB template 1KA1 based on UniProtKB P32179.
ModBase	Search...
Organism-specific databases
Gramene	Q40639.
Phylogenomic databases
OMA	P0C5A3. DGAIYLR.
Enzyme and pathway databases
BRENDA	3.1.3.7. 1975.
Family and domain databases
InterPro	IPR006239. Bisphos_HAL2. IPR000760. Inositol_P. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00378. INOSPHPHTASE.
ProDom	PD023420. Inositol_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01330. bisphos_HAL2. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPNP_ORYSA

Accession

Primary (citable) accession number: P0C5A3
Secondary accession number(s): Q40639

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)