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P0C587

- HEM1_ORYSJ

UniProt

P0C587 - HEM1_ORYSJ

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Protein

Glutamyl-tRNA reductase, chloroplastic

Gene

Os10g0502400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351NucleophileBy similarity
Sitei184 – 1841Important for activityBy similarity
Binding sitei194 – 1941SubstrateBy similarity
Binding sitei205 – 2051SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816NADPBy similarity

GO - Molecular functioni

  1. glutamyl-tRNA reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-KW
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductase, chloroplastic (EC:1.2.1.70)
Short name:
GluTR
Gene namesi
Ordered Locus Names:Os10g0502400, LOC_Os10g35840
ORF Names:OSJNBa0078O01.14, OSJNBb0073N24.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 10

Organism-specific databases

GrameneiP0C587.

Subcellular locationi

Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848ChloroplastSequence AnalysisAdd
BLAST
Chaini49 – 537489Glutamyl-tRNA reductase, chloroplasticPRO_0000013313Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiP0C587. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliP0C587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1374Substrate bindingBy similarity
Regioni199 – 2013Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0373.
InParanoidiP0C587.
KOiK02492.
OMAiGLSIHTT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
SUPFAMiSSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C587-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMASTTSATA AGGAFAAAKT RAGSSAAGGG ACARVAAGGR RRSGVVVRCD
60 70 80 90 100
AGVEAQAQAQ AVAKAASVAA LEQFKISADR YMKERSSIAV IGLSVHTAPV
110 120 130 140 150
EMREKLAVAE ELWPRAISEL TSLNHIEEAA VLSTCNRMEI YVVALSWNRG
160 170 180 190 200
IREVVDWMSK KSGIPASELR EHLFMLRDSD ATRHLFEVSA GLDSLVLGEG
210 220 230 240 250
QILAQVKQVV RSGQNSGGLG KNIDRMFKDA ITAGKRVRCE TNISSGAVSV
260 270 280 290 300
SSAAVELALM KLPKSECLSA RMLLIGAGKM GKLVVKHLIA KGCKKVVVVN
310 320 330 340 350
RSVERVDAIR EEMKDIEIVY RPLTEMYEAA AEADVVFTST ASETPLFTKE
360 370 380 390 400
HAEALPAISD AMGGVRLFVD ISVPRNVSAC VSEVGHARVY NVDDLKEVVE
410 420 430 440 450
ANKEDRLRKA MEAQTIITQE LKRFEAWRDS LETVPTIKKL RSYADRIRAS
460 470 480 490 500
ELEKCLQKIG EDALTKKMRR SIEELSTGIV NKLLHGPLQH LRCDGSDSRT
510 520 530
LDETLENMHA LNRMFSLDTE KAIIEQKIKA KVEKSQN
Length:537
Mass (Da):58,419
Last modified:July 24, 2007 - v1
Checksum:i51415C5525E3C640
GO

Sequence cautioni

The sequence AAG13620.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti346 – 3461L → F in AK099393. (PubMed:12869764)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC078840 Genomic DNA. Translation: AAG13620.1. Different initiation.
AC079888 Genomic DNA. Translation: AAM93670.1.
DP000086 Genomic DNA. Translation: ABB47844.1.
AP008216 Genomic DNA. Translation: BAF26905.1.
AK099393 mRNA. No translation available.
RefSeqiNP_001064991.1. NM_001071526.1.
UniGeneiOs.11129.

Genome annotation databases

EnsemblPlantsiOS10T0502400-01; OS10T0502400-01; OS10G0502400.
GeneIDi4349044.
KEGGiosa:4349044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC078840 Genomic DNA. Translation: AAG13620.1 . Different initiation.
AC079888 Genomic DNA. Translation: AAM93670.1 .
DP000086 Genomic DNA. Translation: ABB47844.1 .
AP008216 Genomic DNA. Translation: BAF26905.1 .
AK099393 mRNA. No translation available.
RefSeqi NP_001064991.1. NM_001071526.1.
UniGenei Os.11129.

3D structure databases

ProteinModelPortali P0C587.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS10T0502400-01 ; OS10T0502400-01 ; OS10G0502400 .
GeneIDi 4349044.
KEGGi osa:4349044.

Organism-specific databases

Gramenei P0C587.

Phylogenomic databases

eggNOGi COG0373.
InParanoidi P0C587.
KOi K02492.
OMAi GLSIHTT.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00316 .

Gene expression databases

ExpressionAtlasi P0C587. baseline and differential.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00087. Glu_tRNA_reductase.
InterProi IPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view ]
Pfami PF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view ]
SUPFAMi SSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsi TIGR01035. hemA. 1 hit.
PROSITEi PS00747. GLUTR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "In-depth view of structure, activity, and evolution of rice chromosome 10."
    Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H.
    , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.
    Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiHEM1_ORYSJ
AccessioniPrimary (citable) accession number: P0C587
Secondary accession number(s): O48674
, Q0IWL0, Q337F6, Q8LNE9, Q9FW00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3