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Protein

Glutamyl-tRNA reductase, chloroplastic

Gene

Os10g0502400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathway:iprotoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase, chloroplastic (Os10g0502400), Glutamyl-tRNA reductase (OsJ_32069)
  2. Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic (GSA)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351NucleophileBy similarity
Sitei184 – 1841Important for activityBy similarity
Binding sitei194 – 1941SubstrateBy similarity
Binding sitei205 – 2051SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductase, chloroplastic (EC:1.2.1.70)
Short name:
GluTR
Gene namesi
Ordered Locus Names:Os10g0502400, LOC_Os10g35840
ORF Names:OSJNBa0078O01.14, OSJNBb0073N24.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 10

Organism-specific databases

GrameneiP0C587.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848ChloroplastSequence AnalysisAdd
BLAST
Chaini49 – 537489Glutamyl-tRNA reductase, chloroplasticPRO_0000013313Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiP0C587. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os10g35840.1.

Structurei

3D structure databases

ProteinModelPortaliP0C587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1374Substrate bindingBy similarity
Regioni199 – 2013Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0373.
InParanoidiP0C587.
KOiK02492.
OMAiNHCPNIK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
SUPFAMiSSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMASTTSATA AGGAFAAAKT RAGSSAAGGG ACARVAAGGR RRSGVVVRCD
60 70 80 90 100
AGVEAQAQAQ AVAKAASVAA LEQFKISADR YMKERSSIAV IGLSVHTAPV
110 120 130 140 150
EMREKLAVAE ELWPRAISEL TSLNHIEEAA VLSTCNRMEI YVVALSWNRG
160 170 180 190 200
IREVVDWMSK KSGIPASELR EHLFMLRDSD ATRHLFEVSA GLDSLVLGEG
210 220 230 240 250
QILAQVKQVV RSGQNSGGLG KNIDRMFKDA ITAGKRVRCE TNISSGAVSV
260 270 280 290 300
SSAAVELALM KLPKSECLSA RMLLIGAGKM GKLVVKHLIA KGCKKVVVVN
310 320 330 340 350
RSVERVDAIR EEMKDIEIVY RPLTEMYEAA AEADVVFTST ASETPLFTKE
360 370 380 390 400
HAEALPAISD AMGGVRLFVD ISVPRNVSAC VSEVGHARVY NVDDLKEVVE
410 420 430 440 450
ANKEDRLRKA MEAQTIITQE LKRFEAWRDS LETVPTIKKL RSYADRIRAS
460 470 480 490 500
ELEKCLQKIG EDALTKKMRR SIEELSTGIV NKLLHGPLQH LRCDGSDSRT
510 520 530
LDETLENMHA LNRMFSLDTE KAIIEQKIKA KVEKSQN
Length:537
Mass (Da):58,419
Last modified:July 24, 2007 - v1
Checksum:i51415C5525E3C640
GO

Sequence cautioni

The sequence AAG13620.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti346 – 3461L → F in AK099393 (PubMed:12869764).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC078840 Genomic DNA. Translation: AAG13620.1. Different initiation.
AC079888 Genomic DNA. Translation: AAM93670.1.
DP000086 Genomic DNA. Translation: ABB47844.1.
AP008216 Genomic DNA. Translation: BAF26905.1.
AK099393 mRNA. No translation available.
RefSeqiNP_001064991.1. NM_001071526.1.
UniGeneiOs.11129.

Genome annotation databases

EnsemblPlantsiOS10T0502400-01; OS10T0502400-01; OS10G0502400.
GeneIDi4349044.
KEGGiosa:4349044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC078840 Genomic DNA. Translation: AAG13620.1. Different initiation.
AC079888 Genomic DNA. Translation: AAM93670.1.
DP000086 Genomic DNA. Translation: ABB47844.1.
AP008216 Genomic DNA. Translation: BAF26905.1.
AK099393 mRNA. No translation available.
RefSeqiNP_001064991.1. NM_001071526.1.
UniGeneiOs.11129.

3D structure databases

ProteinModelPortaliP0C587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os10g35840.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS10T0502400-01; OS10T0502400-01; OS10G0502400.
GeneIDi4349044.
KEGGiosa:4349044.

Organism-specific databases

GrameneiP0C587.

Phylogenomic databases

eggNOGiCOG0373.
InParanoidiP0C587.
KOiK02492.
OMAiNHCPNIK.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.

Gene expression databases

ExpressionAtlasiP0C587. baseline and differential.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
SUPFAMiSSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "In-depth view of structure, activity, and evolution of rice chromosome 10."
    Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H.
    , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.
    Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiHEM1_ORYSJ
AccessioniPrimary (citable) accession number: P0C587
Secondary accession number(s): O48674
, Q0IWL0, Q337F6, Q8LNE9, Q9FW00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: July 22, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.