Catalase-peroxidase - Mycobacterium smegmatis

Preferred order of sections

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:

katG

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria By similarity. HAMAP MF_01961
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O. HAMAP MF_01961 2 H₂O₂ = O₂ + 2 H₂O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 740

740

Catalase-peroxidase HAMAP MF_01961

PRO_0000055573

Sites

Active site

114

1

Proton acceptor By similarity

Metal binding

278

1

Iron (heme axial ligand) By similarity

Site

110

1

Transition state stabilizer By similarity

Amino acid modifications

Cross-link

113 ↔ 237

Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-263) By similarity

Cross-link

237 ↔ 263

Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P0C580 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 397CF9B5AA7E1AE7
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FASTA

740

81,230

        10         20         30         40         50         60 
MPEDRPIEDS PPIGEAQTDA PAGGCPAGFG RIKPPVAGGS NXDWWPNQLN MKILQKNPDV 

        70         80         90        100        110        120 
INPLDEDFDY RSAVQNLDVD ALRADIVEVM HTSQDWWPAD FGHYGPLFIR MAWHAAGTYR 

       130        140        150        160        170        180 
VSDGRGGAGA GMQRFAPLNS WPDNASLDKA RRLLWPVKKK YGKNLSWADL IVYAGNVALE 

       190        200        210        220        230        240 
DMGFRTAGFA FGREDRWEPE EDVYWGPEQE WLDRTKRYTG ERDLENPLAA VQMGLIYVNP 

       250        260        270        280        290        300 
EGPNGNPDPQ ASAIDIRETF GRMAMNDVET AALIVGGHTF GKTHGNGDAS LVGPEPEAAP 

       310        320        330        340        350        360 
LEEVGLGWRN PQGTGVGKDA ITSGLEVTWT HTPTKWDNSF LEILYGNEWE LTKSPAGANQ 

       370        380        390        400        410        420 
WKPKDNGWAN SVPLAHEDGK THPSMLTSDL ALRVDPIYEQ ITRRWLDHPE ELAEEFAKAW 

       430        440        450        460        470        480 
FKLLHRDMGP VTRYLGPEVP KDTWLWQDNI PAGNDLSDDE VAKLKELIAD SGLTVSQLVS 

       490        500        510        520        530        540 
TAWKAASTFR SSDLRGGANG GRIRLQPQLG WEANEPDELA QVVRKYEEIQ KASGINVSFA 

       550        560        570        580        590        600 
DLVVLGGNVG VEKAAKAAGF DVTVPFTPGR GDATQEETDV DSFAYLEPKA DGFRNYLGKG 

       610        620        630        640        650        660 
SDLPAEFKLI DRANLLGLSA PEMTTLVGGL RVLDVNHGGT KHGVLTDKPG ALTTDFFVNL 

       670        680        690        700        710        720 
LDMSTAWKPS PADDGTYIGT DRATGSPKWT GTRVDLVFAS NSQLRALAEV YAEDDSKEKF 

       730        740 
VKDFVAAWTK VMDADRFDVA

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References

[1]	Engler O., Telenti A. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: mc(2)1216.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46844 Genomic DNA. Translation: AAC45275.1.
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	2435. MsmCP01_imm30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCSM

Accession

Primary (citable) accession number: P0C580
Secondary accession number(s): O05763, Q59557

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	October 19, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families