Reviewed, UniProtKB/Swiss-Prot P0C580 (KATG_MYCSM)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:

katG

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Catalase-peroxidase HAMAP MF_01961		PRO_0000055573
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	278	1	Iron (heme axial ligand) By similarity
	Site	110	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	113 ↔ 237		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-263) By similarity
	Cross-link	237 ↔ 263		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C580-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 397CF9B5AA7E1AE7	FASTA	740	81,230
	10 20 30 40 50 60 MPEDRPIEDS PPIGEAQTDA PAGGCPAGFG RIKPPVAGGS NXDWWPNQLN MKILQKNPDV 70 80 90 100 110 120 INPLDEDFDY RSAVQNLDVD ALRADIVEVM HTSQDWWPAD FGHYGPLFIR MAWHAAGTYR 130 140 150 160 170 180 VSDGRGGAGA GMQRFAPLNS WPDNASLDKA RRLLWPVKKK YGKNLSWADL IVYAGNVALE 190 200 210 220 230 240 DMGFRTAGFA FGREDRWEPE EDVYWGPEQE WLDRTKRYTG ERDLENPLAA VQMGLIYVNP 250 260 270 280 290 300 EGPNGNPDPQ ASAIDIRETF GRMAMNDVET AALIVGGHTF GKTHGNGDAS LVGPEPEAAP 310 320 330 340 350 360 LEEVGLGWRN PQGTGVGKDA ITSGLEVTWT HTPTKWDNSF LEILYGNEWE LTKSPAGANQ 370 380 390 400 410 420 WKPKDNGWAN SVPLAHEDGK THPSMLTSDL ALRVDPIYEQ ITRRWLDHPE ELAEEFAKAW 430 440 450 460 470 480 FKLLHRDMGP VTRYLGPEVP KDTWLWQDNI PAGNDLSDDE VAKLKELIAD SGLTVSQLVS 490 500 510 520 530 540 TAWKAASTFR SSDLRGGANG GRIRLQPQLG WEANEPDELA QVVRKYEEIQ KASGINVSFA 550 560 570 580 590 600 DLVVLGGNVG VEKAAKAAGF DVTVPFTPGR GDATQEETDV DSFAYLEPKA DGFRNYLGKG 610 620 630 640 650 660 SDLPAEFKLI DRANLLGLSA PEMTTLVGGL RVLDVNHGGT KHGVLTDKPG ALTTDFFVNL 670 680 690 700 710 720 LDMSTAWKPS PADDGTYIGT DRATGSPKWT GTRVDLVFAS NSQLRALAEV YAEDDSKEKF 730 740 VKDFVAAWTK VMDADRFDVA

References

[1]	Engler O., Telenti A. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: mc(2)1216.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U46844 Genomic DNA. Translation: AAC45275.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.11.1.6. 259.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCSM

Accession

Primary (citable) accession number: P0C580
Secondary accession number(s): O05763, Q59557

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 5, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)