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P0C574

- PB1F2_I18A0

UniProt

P0C574 - PB1F2_I18A0

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Protein
Protein PB1-F2
Gene
PB1
Organism
Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis By similarity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661Important for pathogenicity, S is highly pathogenic whereas N is low pathogenic

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. modulation by virus of host apoptotic process Source: UniProtKB-KW
  3. suppression by virus of host MAVS activity Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Modulation of host cell apoptosis by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PB1-F2
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
Taxonomic identifieri88776 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008430: Genome

Subcellular locationi

Host mitochondrion inner membrane By similarity. Host nucleus By similarity. Host cytoplasmhost cytosol By similarity
Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol By similarity.

GO - Cellular componenti

  1. host cell cytosol Source: UniProtKB-SubCell
  2. host cell mitochondrial inner membrane Source: UniProtKB-SubCell
  3. host cell nucleus Source: UniProtKB-SubCell
  4. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Host mitochondrion, Host mitochondrion inner membrane, Host nucleus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi66 – 661S → N: Reduces 50% lethal dose by 3-log in mice. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9090Protein PB1-F2
PRO_0000310572Add
BLAST

Interactioni

Subunit structurei

Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C574.
SMRiP0C574. Positions 50-87.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 8723Mitochondrial targeting sequence By similarity
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view]
PfamiPF11986. PB1-F2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C574-1 [UniParc]FASTAAdd to Basket

« Hide

MGQEQDTPWI LSTGHISTQK REDGQQTPRL EHHNSTRLMD HCQKTMNQVV   50
MPKQIVYWKQ WLSLRSPTPV SLKTRVLKRW RLFSKHEWTS 90
Length:90
Mass (Da):10,810
Last modified:November 13, 2007 - v1
Checksum:i81FAE70341B06964
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ208310 mRNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ208310 mRNA. No translation available.

3D structure databases

ProteinModelPortali P0C574.
SMRi P0C574. Positions 50-87.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view ]
Pfami PF11986. PB1-F2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the 1918 influenza virus polymerase genes."
    Taubenberger J.K., Reid A.H., Lourens R.M., Wang R., Jin G., Fanning T.G.
    Nature 437:889-893(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 Influenza A viruses contributes to increased virulence."
    Conenello G.M., Zamarin D., Perrone L.A., Tumpey T., Palese P.
    PLoS Pathog. 3:1414-1421(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-66.
  3. "Expression of the 1918 Influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia."
    McAuley J.L., Hornung F., Boyd K.L., Smith A.M., McKeon R., Bennick J., Yewdell J.W., McCullers J.A.
    Cell Host Microbe 2:240-249(2007)
    Cited for: ROLE IN PATHOGENICITY.

Entry informationi

Entry nameiPB1F2_I18A0
AccessioniPrimary (citable) accession number: P0C574
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Is not encoded in all strains, and seems to be dispensable for replication.
South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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