P0C574 (PB1F2_I18A0) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 24. History...
Names and origin
|Protein names||Recommended name:|
|Organism||Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1)) [Complete proteome]|
|Taxonomic identifier||88776 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›|
|Virus host||Aves [TaxID: 8782]|
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
|Sequence length||90 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis By similarity. Ref.3
Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1 By similarity.
Host mitochondrion inner membrane By similarity. Host nucleus By similarity. Host cytoplasm › host cytosol By similarity. Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol By similarity.
Is not encoded in all strains, and seems to be dispensable for replication.
South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.
Belongs to the influenza viruses PB1-F2 family.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 90||90||Protein PB1-F2||PRO_0000310572|
|Region||65 – 87||23||Mitochondrial targeting sequence By similarity|
|Site||66||1||Important for pathogenicity, S is highly pathogenic whereas N is low pathogenic|
|Mutagenesis||66||1||S → N: Reduces 50% lethal dose by 3-log in mice. Ref.2|
|||"Characterization of the 1918 influenza virus polymerase genes."|
Taubenberger J.K., Reid A.H., Lourens R.M., Wang R., Jin G., Fanning T.G.
Nature 437:889-893(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 Influenza A viruses contributes to increased virulence."|
Conenello G.M., Zamarin D., Perrone L.A., Tumpey T., Palese P.
PLoS Pathog. 3:1414-1421(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF SER-66.
|||"Expression of the 1918 Influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia."|
McAuley J.L., Hornung F., Boyd K.L., Smith A.M., McKeon R., Bennick J., Yewdell J.W., McCullers J.A.
Cell Host Microbe 2:240-249(2007)
Cited for: ROLE IN PATHOGENICITY.
|DQ208310 mRNA. No translation available.|
3D structure databases
|SMR||P0C574. Positions 50-87. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR021045. Flu_proapoptotic_PB1-F2. |
|Pfam||PF11986. PB1-F2. 1 hit. |
|Accession||Primary (citable) accession number: P0C574|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families