Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0C574

- PB1F2_I18A0

UniProt

P0C574 - PB1F2_I18A0

Protein

Protein PB1-F2

Gene

PB1

Organism
Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei66 – 661Important for pathogenicity, S is highly pathogenic whereas N is low pathogenic

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. modulation by virus of host apoptotic process Source: UniProtKB-KW
    3. suppression by virus of host MAVS activity Source: UniProtKB

    Keywords - Biological processi

    Apoptosis, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Modulation of host cell apoptosis by virus, Viral immunoevasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein PB1-F2
    Gene namesi
    Name:PB1
    OrganismiInfluenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
    Taxonomic identifieri88776 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008430: Genome

    Subcellular locationi

    Host mitochondrion inner membrane By similarity. Host nucleus By similarity. Host cytoplasmhost cytosol By similarity
    Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol By similarity.By similarity

    GO - Cellular componenti

    1. host cell cytosol Source: UniProtKB-SubCell
    2. host cell mitochondrial inner membrane Source: UniProtKB-SubCell
    3. host cell nucleus Source: UniProtKB-SubCell
    4. membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Host mitochondrion, Host mitochondrion inner membrane, Host nucleus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi66 – 661S → N: Reduces 50% lethal dose by 3-log in mice. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9090Protein PB1-F2PRO_0000310572Add
    BLAST

    Interactioni

    Subunit structurei

    Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C574.
    SMRiP0C574. Positions 50-87.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni65 – 8723Mitochondrial targeting sequenceBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the influenza viruses PB1-F2 family.Curated

    Family and domain databases

    InterProiIPR021045. Flu_proapoptotic_PB1-F2.
    [Graphical view]
    PfamiPF11986. PB1-F2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C574-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQEQDTPWI LSTGHISTQK REDGQQTPRL EHHNSTRLMD HCQKTMNQVV   50
    MPKQIVYWKQ WLSLRSPTPV SLKTRVLKRW RLFSKHEWTS 90
    Length:90
    Mass (Da):10,810
    Last modified:November 13, 2007 - v1
    Checksum:i81FAE70341B06964
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ208310 mRNA. No translation available.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ208310 mRNA. No translation available.

    3D structure databases

    ProteinModelPortali P0C574.
    SMRi P0C574. Positions 50-87.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR021045. Flu_proapoptotic_PB1-F2.
    [Graphical view ]
    Pfami PF11986. PB1-F2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the 1918 influenza virus polymerase genes."
      Taubenberger J.K., Reid A.H., Lourens R.M., Wang R., Jin G., Fanning T.G.
      Nature 437:889-893(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 Influenza A viruses contributes to increased virulence."
      Conenello G.M., Zamarin D., Perrone L.A., Tumpey T., Palese P.
      PLoS Pathog. 3:1414-1421(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF SER-66.
    3. "Expression of the 1918 Influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia."
      McAuley J.L., Hornung F., Boyd K.L., Smith A.M., McKeon R., Bennick J., Yewdell J.W., McCullers J.A.
      Cell Host Microbe 2:240-249(2007)
      Cited for: ROLE IN PATHOGENICITY.

    Entry informationi

    Entry nameiPB1F2_I18A0
    AccessioniPrimary (citable) accession number: P0C574
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Is not encoded in all strains, and seems to be dispensable for replication.
    South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3