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P0C574 (PB1F2_I18A0) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length90 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in promoting lung pathology in both primary viral infection and secondary bacterial infection. Promotes alteration of mitochondrial morphology, dissipation of mitochondrial membrane potential, and cell death. Alternatively, inhibits the production of interferon in the infected cell at the level of host mitochondrial antiviral signaling MAVS. Its level of expression differs greatly depending on which cell type is infected, in a manner that is independent of the levels of expression of other viral proteins. Monocytic cells are more affected than epithelial cells. Seems to disable virus-infected monocytes or other host innate immune cells. May also act in trans: extracellular PB1-F2 released by infected cells could potentially inactivate hosts cell recruitment to the site of infection. During early stage of infection, may predispose the mitochondria to permeability transition through interaction with human SLC25A6/ANT3 and VDAC1. These proteins participate in the formation of the permeability transition pore complex (PTPC) responsible of the release of mitochondrial products that triggers apoptosis By similarity. Ref.3

Subunit structure

Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1 By similarity.

Subcellular location

Host mitochondrion inner membrane By similarity. Host nucleus By similarity. Host cytoplasmhost cytosol By similarity. Note: Inner mitochondrial membrane in most cells types. Otherwise is detected in the nucleus and cytosol By similarity.

Miscellaneous

Is not encoded in all strains, and seems to be dispensable for replication.

South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

Sequence similarities

Belongs to the influenza viruses PB1-F2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9090Protein PB1-F2
PRO_0000310572

Regions

Region65 – 8723Mitochondrial targeting sequence By similarity

Sites

Site661Important for pathogenicity, S is highly pathogenic whereas N is low pathogenic

Experimental info

Mutagenesis661S → N: Reduces 50% lethal dose by 3-log in mice. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P0C574 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 81FAE70341B06964

FASTA9010,810
        10         20         30         40         50         60 
MGQEQDTPWI LSTGHISTQK REDGQQTPRL EHHNSTRLMD HCQKTMNQVV MPKQIVYWKQ 

        70         80         90 
WLSLRSPTPV SLKTRVLKRW RLFSKHEWTS 

« Hide

References

[1]"Characterization of the 1918 influenza virus polymerase genes."
Taubenberger J.K., Reid A.H., Lourens R.M., Wang R., Jin G., Fanning T.G.
Nature 437:889-893(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 Influenza A viruses contributes to increased virulence."
Conenello G.M., Zamarin D., Perrone L.A., Tumpey T., Palese P.
PLoS Pathog. 3:1414-1421(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF SER-66.
[3]"Expression of the 1918 Influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia."
McAuley J.L., Hornung F., Boyd K.L., Smith A.M., McKeon R., Bennick J., Yewdell J.W., McCullers J.A.
Cell Host Microbe 2:240-249(2007)
Cited for: ROLE IN PATHOGENICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ208310 mRNA. No translation available.

3D structure databases

ProteinModelPortalP0C574.
SMRP0C574. Positions 50-87.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR021045. Flu_proapoptotic_PB1-F2.
[Graphical view]
PfamPF11986. PB1-F2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePB1F2_I18A0
AccessionPrimary (citable) accession number: P0C574
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families