ID   L_MOKV                  Reviewed;        2127 AA.
AC   P0C568;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   16-JUN-2009, entry version 14.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Mokola virus (MOKV).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Lyssavirus.
OX   NCBI_TaxID=12538;
OH   NCBI_TaxID=9615; Canis familiaris (Dog).
OH   NCBI_TaxID=9685; Felis silvestris catus (Cat).
OH   NCBI_TaxID=9989; Rodentia.
OH   NCBI_TaxID=269271; Chodsigoa caovansunga (Shrew).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9225031;
RA   Le Mercier P., Jacob Y., Tordo N.;
RT   "The complete Mokola virus genome sequence: structure of the RNA-
RT   dependent RNA polymerase.";
RL   J. Gen. Virol. 78:1571-1576(1997).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein to form the functional
CC       polymerase (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviruses protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; Y09762; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   RefSeq; YP_142354.1; -.
DR   GeneID; 3159474; -.
DR   BRENDA; 2.1.1.56; 295491.
DR   BRENDA; 2.7.7.48; 295491.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017234; RNA-dir_RNA_pol_rhabdovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Methyltransferase;
KW   mRNA capping; mRNA processing; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN         1   2127       Large structural protein.
FT                                /FTId=PRO_0000294416.
FT   DOMAIN      611    799       RdRp catalytic.
FT   REGION     1562   2127       Interaction with P protein (By
FT                                similarity).
SQ   SEQUENCE   2127 AA;  242160 MW;  ACE7284E16A95328 CRC64;
     MMDVTEVYDD PIDPVEPEGE WNSSPVVPNI LRNSDYNLNS PLLEDPANLM IQWLTSGNRP
     SRMNVTENTT RSYKVLRALF KGVDIATIKI GGVGAQAMMG LWVLGSHSES SRSRKCLADL
     SAFYQRTLPI ESILNHTLMN RGLQTPREGV LSGLNRVSYD QSFGRYLGNL YSSYLLFHVI
     ILYMNALDWE EEKTILALWR DITSIDIKND RVYFKDPLWG KLLVTKDFVY AHNSNCLFDK
     NYTLMLKDLF LSRFNSLLIL VSPPDSRYSD DLAANLCRLY ISGDRLLSSC GNAGYDVIKM
     LEPCVVDLLV QRAETFRPLI HSLGEFPAFI KDKTTQLIGT FGPCASQFFS MLQQFDNIHD
     LVFIYGCYRH WGHPYIDYRK GLSKLFDQVH MKKTIDQQYQ ERLASDLARK ILRWGFEKYS
     KWYLDTGVIP KDHPLAPYIA TQTWPPKHVV DLLGDSWHTL PMTQIFEVPE SMDPSEILDD
     KSHSFTRTKL SSWSSEHRGG PVPSEKVIIT ALSRPPVNPR DFLKSIDQGG LPDDDLIIGL
     KPKERELKID GRFFALMSWN LRLYFVITEK LLANHIIPLF DALTMTDNLN KVFKKLIDRV
     TGQGLKDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDR AFGMKKVFSR THEFFQKSWI
     YYSDRSDLIG IWKDQIYCLD MTEGPTCWNG QDGGLEGLRQ KGWSLVSLLM IERESKTRNT
     RTKILAQGDN QVLCPTYMLS SGLNNEGLRY ELENISKNAM SIYRAIEDGA SKLGLIIKKE
     ETMCSFDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH
     SQSLVKPMRD FLLMSVQAIY HYLLFSPIIK DRVYKVLNSK DDDFLLAMSR IIYLDPSLGG
     VSGMSLGRFP IRQFSDPVSE GLTFWKEIWL SSSETWIHHL CQEAGNPDLG DRSLESFTRL
     LEDPTTLNIR GGASPTILLK EAIRKALYDE VDRVENSEFR EAIILSKTHR DNFILFLKSI
     EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTVRRQFRKS LSKTLEESFL NSEIHGINRV
     TQTPQRLGRV WTCSAERADQ LREISWGRKV VGTTVPNPSE MLTLVPKSSV ACGCYTREVG
     NPRISVSVLP SFDPSFLSRG PLKGYLGSST SMSTQLFHSW EKVTNVHVVK RALSLKESIN
     WFVSRESNLA KTLIGNILSL TGPSFPIEEA PVFKRTGSAL HRFKSARYSE GGYSAVCPNL
     LSHISVSTDT MSDLTQDGTN FDFMFQPLML YAQTWTSELV QKDFRLADST FHWHLRCQKC
     IRPIEEVTLD APQLFDFPDI SSRISRMVSG AVPQFRKLPE VGLKAGDLTA LSSSERSYHI
     GTAQGLLYSI LVAVHDPGYN DNSLFPVNIY GKVSARGYLR GLARGILIGS SICFLTRMTN
     ININRPLELI SGVISYILLK LDNHPSLYIM LKEPELRAEI FSIPQKVPAA YPTTMEEGNR
     SVLCYLQQVL RYERDSMSFP PGNDILWIFS DFRSIKMTYL TLITFQAYLW LQRVERNLSK
     QVRVRLRQLN SLMRQVLGGH GEDTIDSDDE ILSLLKESLR RTRWVDQEVR HAAKSMTPDL
     NPVPKISRRM GSSEWICSAQ QIAISTSLNP ASASDIDLRS LSRQYQNPLI SGLRVVQWAT
     GAHYKIKPIL NDLDVCPCLS LVIGDGSGGI SRVVLSMFPD SKLVFNSLLE VNDLMASGTH
     PLPPSALMRG GDDITSRVID FESIWEKPSD LRNPLTWKYF HSIQSKLRSQ FDLIVCDAEV
     TDIESVNKIT LLLSDFSMSI KGPLYLIFKT YGTMLVNPDY KAIHHLSRAF PNVTGFVTQM
     TSSFSSEIYL RFSKTGYFFR DHELLTASTV REMSLVLFNC SNPKSEMLRA RTLNYQDLIR
     GFPPEIISNP YNEMIITLID SEVESFLVHK VVDDLELKRG APSKMAIIIA VAILFSNSVL
     NVSKSLNEPK FFPPSDPKLL RHFNICSSTL LFLSTALGDL SNFTRLHELY NSPVTYYFGK
     QTIKGRRYLS WSWANSSPIF KKVACNSSIS LSSHWIRLIY KIVKTTRLNC SPRDMLRETE
     ACLRTYNKWI NIRDTRSRTS ILDYCCL
//