P0C384 (NDHI_ORYSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(P)H-quinone oxidoreductase subunit I, chloroplastic
EC=1.6.5.-

Alternative name(s):
NAD(P)H dehydrogenase subunit I
Short name=NDH subunit I
NADH-plastoquinone oxidoreductase subunit I

Gene names

Name:	ndhI
Synonyms:	frxB
ORF Names:	PA173

Encoded on

Plastid; Chloroplast

Organism

Oryza sativa (Rice)

Taxonomic identifier

4530 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01351
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP-Rule MF_01351
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP-Rule MF_01351
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_01351.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological_process	photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid Inferred by curator PubMed 12520024. Source: Gramene
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	NAD(P)H-quinone oxidoreductase subunit I, chloroplastic HAMAP-Rule MF_01351		PRO_0000118711
Regions
	Domain	55 – 84	30	4Fe-4S ferredoxin-type 1
	Domain	95 – 124	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	64	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	67	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	70	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	74	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	104	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	107	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	110	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	114	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C384 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 58F13F2645FE25BB	FASTA	180	21,165
	10 20 30 40 50 60 MFPMVTGFMS YGQQTIRAAR YIGQSFIITL SHTNRLPITI HYPYEKSITS ERFRGRIHFE 70 80 90 100 110 120 FDKCIACEVC VRVCPIDLPL VDWRFEKDIK RKQLLNYSID FGVCIFCGNC VEYCPTNCLS 130 140 150 160 170 180 MTEEYELSTY DRHELNYNQI ALSRLPISIM GDYTIQTIRN STQSKIDEEK SWNSRTITDY

References

[1]	"A comparison of rice chloroplast genomes." Tang J., Xia H., Cao M., Zhang X., Zeng W., Hu S., Tong W., Wang J., Wang J., Yu J., Yang H., Zhu L. Plant Physiol. 135:412-420(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. PA64s.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY522331 Genomic DNA. Translation: AAS46224.1.
3D structure databases
ProteinModelPortal	P0C384.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P0C384.
Phylogenomic databases
HOGENOM	HOG000276870.
Gene expression databases
ArrayExpress	P0C384.
Genevestigator	P0C384.
Family and domain databases
HAMAP	MF_01351. NDH1_NuoI.
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR004497. NADH_plast_OxRdtase_su_I. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR00403. ndhI. 1 hit. TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDHI_ORYSA

Accession

Primary (citable) accession number: P0C384
Secondary accession number(s): P12099, Q6QXX0, Q6QY33

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program