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Protein

Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

Gene

Aldh4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes (By similarity).By similarity

Catalytic activityi

L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable proline dehydrogenase 2 (Prodh2)
  2. Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (Aldh4a1)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei207NADBy similarity1
Sitei210Transition state stabilizerBy similarity1
Binding sitei232NADBy similarity1
Active sitei313Proton acceptorPROSITE-ProRule annotation1
Active sitei347NucleophilePROSITE-ProRule annotation1
Binding sitei446NADBy similarity1
Binding sitei512SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 289NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Proline metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP0C2X9.
UniPathwayiUPA00261; UER00374.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (EC:1.2.1.88)
Short name:
P5C dehydrogenase
Alternative name(s):
Aldehyde dehydrogenase family 4 member A1
L-glutamate gamma-semialdehyde dehydrogenase
Gene namesi
Name:Aldh4a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1624206. Aldh4a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionBy similarityAdd BLAST23
ChainiPRO_000028782724 – 563Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrialAdd BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30N6-succinyllysineBy similarity1
Modified residuei43PhosphoserineBy similarity1
Modified residuei51N6-acetyllysineBy similarity1
Modified residuei92N6-acetyllysine; alternateBy similarity1
Modified residuei92N6-succinyllysine; alternateBy similarity1
Modified residuei98N6-acetyllysine; alternateBy similarity1
Modified residuei98N6-succinyllysine; alternateBy similarity1
Modified residuei113N6-acetyllysine; alternateBy similarity1
Modified residuei113N6-succinyllysine; alternateBy similarity1
Modified residuei129N6-acetyllysine; alternateBy similarity1
Modified residuei129N6-succinyllysine; alternateBy similarity1
Modified residuei174N6-acetyllysine; alternateBy similarity1
Modified residuei174N6-succinyllysine; alternateBy similarity1
Modified residuei317N6-acetyllysineBy similarity1
Modified residuei346N6-succinyllysineBy similarity1
Modified residuei364N6-acetyllysineBy similarity1
Modified residuei375N6-acetyllysineBy similarity1
Modified residuei394N6-succinyllysineBy similarity1
Modified residuei461N6-acetyllysineBy similarity1
Modified residuei508N6-acetyllysine; alternateBy similarity1
Modified residuei508N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP0C2X9.

PTM databases

iPTMnetiP0C2X9.
PhosphoSitePlusiP0C2X9.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C2X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000271511.
HOVERGENiHBG050484.
InParanoidiP0C2X9.
PhylomeDBiP0C2X9.

Family and domain databases

CDDicd07123. ALDH_F4-17_P5CDH. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005931. P5CDH/ALDH4A1.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01236. D1pyr5carbox1. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPPALLRRS LLSYAWRGSG LRWKHASSLK VANEPILAFT QGSPERDALQ
60 70 80 90 100
KALNDLKDQT EAIPCVVGDE EVWTSDVRYQ LSPFNHGHKV AKFCYADKAL
110 120 130 140 150
LNKAIEAAVL ARKEWDLKPV ADRAQIFLKA ADMLSGPRRA EILAKTMVGQ
160 170 180 190 200
GKTVIQAEID AAAELIDFFR FNAKFAVELE GEQPISVPPS TNHVVYRGLE
210 220 230 240 250
GFVAAISPFN FTAIGGNLAG APALMGNVVL WKPSDTAMLA SYAVYRILRE
260 270 280 290 300
AGLPPNVIQF VPADGPTFGD TVTSSEHLCG INFTGSVPTF KHLWKQVAQN
310 320 330 340 350
LDRFRTFPRL AGECGGKNFH FVHSSADVDS VVSGTLRSAF EYGGQKCSAC
360 370 380 390 400
SRLYVPQSLW PQIKGRLLEE HSRIKVGNPA EDFGTFFSAV IDAKAFARIK
410 420 430 440 450
KWLEHARSSP SLSILAGGQC NESVGYFVEP CIIESKDPQE PIMKEEIFGP
460 470 480 490 500
VLTVYVYPDE KYRETLQLVD STTSYGLTGA VFAQDKTIVQ EATRMLRNAA
510 520 530 540 550
GNFYINDKST GSVVGQQPFG GARASGERDI PGQPRLVQLW TEPPFTPLAV
560
SPPLGDWRYS YMQ
Length:563
Mass (Da):61,869
Last modified:May 15, 2007 - v1
Checksum:iAF19417D93825EA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03107656 Genomic DNA. No translation available.
UniGeneiRn.203318.

Genome annotation databases

UCSCiRGD:1624206. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03107656 Genomic DNA. No translation available.
UniGeneiRn.203318.

3D structure databases

ProteinModelPortaliP0C2X9.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP0C2X9.
PhosphoSitePlusiP0C2X9.

Proteomic databases

PRIDEiP0C2X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:1624206. rat.

Organism-specific databases

RGDi1624206. Aldh4a1.

Phylogenomic databases

HOGENOMiHOG000271511.
HOVERGENiHBG050484.
InParanoidiP0C2X9.
PhylomeDBiP0C2X9.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00374.
SABIO-RKP0C2X9.

Miscellaneous databases

PROiP0C2X9.

Family and domain databases

CDDicd07123. ALDH_F4-17_P5CDH. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005931. P5CDH/ALDH4A1.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01236. D1pyr5carbox1. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAL4A1_RAT
AccessioniPrimary (citable) accession number: P0C2X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.