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Protein

F-box/SPRY domain-containing protein 1

Gene

FBXO45

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration (By similarity). Plays a role in the regulation of neurotransmission at mature neurons (By similarity). May controls synaptic activity by controlling UNC13A via ubiquitin dependent pathway (By similarity). Specifically recognizes TP73, promoting its ubiquitination and degradation.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/SPRY domain-containing protein 1
Alternative name(s):
F-box only protein 45
Short name:
hFbxo45
Gene namesi
Name:FBXO45
Synonyms:FBX45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29148. FBXO45.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134904114.

Polymorphism and mutation databases

BioMutaiFBXO45.
DMDMi146286173.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 286285F-box/SPRY domain-containing protein 1PRO_0000285933Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0C2W1.
MaxQBiP0C2W1.
PaxDbiP0C2W1.
PRIDEiP0C2W1.

PTM databases

iPTMnetiP0C2W1.
PhosphoSiteiP0C2W1.

Expressioni

Inductioni

Down-regulated in response to DNA-damage.1 Publication

Gene expression databases

BgeeiP0C2W1.
CleanExiHS_FBXO45.
ExpressionAtlasiP0C2W1. baseline and differential.
GenevisibleiP0C2W1. HS.

Organism-specific databases

HPAiHPA040730.

Interactioni

Subunit structurei

Forms a complex with MYCBP2 and SKP1 but does not appear to form an authentic SCF (SKP1-CUL1-F-box protein) complex (By similarity). Interacts (via B30.2/SPRY domain) with TP73 (via SAM domain); leading to TP73 ubiquitination and degradation by proteasome. Interacts (via SRY domain) with UNC13A; leading to degradation of UNC13A by the proteasome (By similarity).By similarity

Protein-protein interaction databases

BioGridi128358. 20 interactions.
IntActiP0C2W1. 5 interactions.
STRINGi9606.ENSP00000310332.

Structurei

3D structure databases

ProteinModelPortaliP0C2W1.
SMRiP0C2W1. Positions 111-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 8250F-boxPROSITE-ProRule annotationAdd
BLAST
Domaini92 – 284193B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the FBXO45/Fsn family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG056500.
InParanoidiP0C2W1.
KOiK10319.
OMAiNNDVWRL.
OrthoDBiEOG7QZGBV.
PhylomeDBiP0C2W1.
TreeFamiTF312822.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001810. F-box_dom.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2W1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAPGAGA ASGGAGCSGG GAGAGAGSGS GAAGAGGRLP SRVLELVFSY
60 70 80 90 100
LELSELRSCA LVCKHWYRCL HGDENSEVWR SLCARSLAEE ALRTDILCNL
110 120 130 140 150
PSYKAKIRAF QHAFSTNDCS RNVYIKKNGF TLHRNPIAQS TDGARTKIGF
160 170 180 190 200
SEGRHAWEVW WEGPLGTVAV IGIATKRAPM QCQGYVALLG SDDQSWGWNL
210 220 230 240 250
VDNNLLHNGE VNGSFPQCNN APKYQIGERI RVILDMEDKT LAFERGYEFL
260 270 280
GVAFRGLPKV CLYPAVSAVY GNTEVTLVYL GKPLDG
Length:286
Mass (Da):30,633
Last modified:May 1, 2007 - v1
Checksum:iC2CF11ED007CC837
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC092933 Genomic DNA. No translation available.
CH471191 Genomic DNA. Translation: EAW53643.1.
CH471191 Genomic DNA. Translation: EAW53644.1.
AK025697 mRNA. No translation available.
CCDSiCCDS46985.1.
RefSeqiNP_001099043.1. NM_001105573.1.
UniGeneiHs.169815.

Genome annotation databases

EnsembliENST00000311630; ENSP00000310332; ENSG00000174013.
GeneIDi200933.
KEGGihsa:200933.
UCSCiuc010iai.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC092933 Genomic DNA. No translation available.
CH471191 Genomic DNA. Translation: EAW53643.1.
CH471191 Genomic DNA. Translation: EAW53644.1.
AK025697 mRNA. No translation available.
CCDSiCCDS46985.1.
RefSeqiNP_001099043.1. NM_001105573.1.
UniGeneiHs.169815.

3D structure databases

ProteinModelPortaliP0C2W1.
SMRiP0C2W1. Positions 111-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128358. 20 interactions.
IntActiP0C2W1. 5 interactions.
STRINGi9606.ENSP00000310332.

PTM databases

iPTMnetiP0C2W1.
PhosphoSiteiP0C2W1.

Polymorphism and mutation databases

BioMutaiFBXO45.
DMDMi146286173.

Proteomic databases

EPDiP0C2W1.
MaxQBiP0C2W1.
PaxDbiP0C2W1.
PRIDEiP0C2W1.

Protocols and materials databases

DNASUi200933.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000311630; ENSP00000310332; ENSG00000174013.
GeneIDi200933.
KEGGihsa:200933.
UCSCiuc010iai.4. human.

Organism-specific databases

CTDi200933.
GeneCardsiFBXO45.
HGNCiHGNC:29148. FBXO45.
HPAiHPA040730.
MIMi609112. gene.
neXtProtiNX_P0C2W1.
PharmGKBiPA134904114.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG056500.
InParanoidiP0C2W1.
KOiK10319.
OMAiNNDVWRL.
OrthoDBiEOG7QZGBV.
PhylomeDBiP0C2W1.
TreeFamiTF312822.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

GenomeRNAii200933.
NextBioi90019.
PROiP0C2W1.
SOURCEiSearch...

Gene expression databases

BgeeiP0C2W1.
CleanExiHS_FBXO45.
ExpressionAtlasiP0C2W1. baseline and differential.
GenevisibleiP0C2W1. HS.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001810. F-box_dom.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 107-286.
    Tissue: Hepatoma.
  4. "Systematic analysis and nomenclature of mammalian F-box proteins."
    Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W.
    Genes Dev. 18:2573-2580(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, IDENTIFICATION.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "The F-box protein FBXO45 promotes the proteasome-dependent degradation of p73."
    Peschiaroli A., Scialpi F., Bernassola F., Pagano M., Melino G.
    Oncogene 28:3157-3166(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, INTERACTION WITH TP73.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFBSP1_HUMAN
AccessioniPrimary (citable) accession number: P0C2W1
Secondary accession number(s): A6NF90, D3DXB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.