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P0C2T8

- AMPN_LACLC

UniProt

P0C2T8 - AMPN_LACLC

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Protein
Aminopeptidase N
Gene
pepN, lap
Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover By similarity.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201Substrate By similarity
Metal bindingi288 – 2881Zinc; catalytic By similarity
Active sitei289 – 2891Proton acceptor By similarity
Metal bindingi292 – 2921Zinc; catalytic By similarity
Metal bindingi311 – 3111Zinc; catalytic By similarity
Sitei375 – 3751Transition state stabilizer By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP0C2T8.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
Synonyms:lap
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

Cytoplasm By similarity
Note: It may be secreted through an unknown mechanism By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 846845Aminopeptidase N
PRO_0000095075Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni252 – 2565Substrate binding By similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2T8-1 [UniParc]FASTAAdd to Basket

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MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVAFHAKGL    50
HFNKVRAFSV DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI 100
YPSYYEVNGE KKMLIGTQFE SHFARQAFPS IDEPEAKATF DLSVKFDEEE 150
GDIIVSNMPE LLNINGIHVF ERTVKMSSYL LAFVFGELQY KKGKTKSGVE 200
VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH SWHIALPDFS 250
SGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 300
MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD 350
GVQSVHVEVT HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL 400
YFKRHQYGNT VGDNLWDALA EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD 450
TLILSQKQFF VGEGVDKGRL WNVPLNTNWT GLPDLLSSEK VEIPGFAALK 500
TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV TKFQILQDRK 550
LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE 600
KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK 650
TKASQIFATH KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS 700
LQEFKRELEG AVALIKDEKV IAELLESFKN ADIVKPQDIA FSWFYLLRND 750
FSQDAAWAWE KANWASLEEK LGGDMSYDKF VIYPGNTFKT ADKLAEYKAF 800
FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL 846
Length:846
Mass (Da):95,309
Last modified:May 1, 2007 - v1
Checksum:i8606417173834D40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61230 Genomic DNA. Translation: CAA43547.1.
PIRiJN0324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61230 Genomic DNA. Translation: CAA43547.1 .
PIRi JN0324.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P0C2T8.

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of a gene (lap) encoding a 95.3-kDa aminopeptidase from Lactococcus lactis ssp. cremoris Wg2."
    Stroman P.
    Gene 113:107-112(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wg2.
  2. "Purification and characterization of an aminopeptidase from Lactococcus lactis subsp. cremoris Wg2."
    Tan P.S.T., Konings W.N.
    Appl. Environ. Microbiol. 56:526-532(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-34.
    Strain: Wg2.

Entry informationi

Entry nameiAMPN_LACLC
AccessioniPrimary (citable) accession number: P0C2T8
Secondary accession number(s): P37897
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: May 14, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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