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P0C2T8

- AMPN_LACLC

UniProt

P0C2T8 - AMPN_LACLC

Protein

Aminopeptidase N

Gene

pepN

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover By similarity.By similarity

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei120 – 1201SubstrateBy similarity
    Metal bindingi288 – 2881Zinc; catalyticPROSITE-ProRule annotation
    Active sitei289 – 2891Proton acceptorPROSITE-ProRule annotation
    Metal bindingi292 – 2921Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi311 – 3111Zinc; catalyticPROSITE-ProRule annotation
    Sitei375 – 3751Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP0C2T8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Alternative name(s):
    Alanine aminopeptidase
    Lysyl aminopeptidase
    Short name:
    Lys-AP
    Gene namesi
    Name:pepN
    Synonyms:lap
    OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
    Taxonomic identifieri1359 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    Subcellular locationi

    Cytoplasm By similarity
    Note: It may be secreted through an unknown mechanism.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 846845Aminopeptidase NPRO_0000095075Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni252 – 2565Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C2T8-1 [UniParc]FASTAAdd to Basket

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    MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVAFHAKGL    50
    HFNKVRAFSV DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI 100
    YPSYYEVNGE KKMLIGTQFE SHFARQAFPS IDEPEAKATF DLSVKFDEEE 150
    GDIIVSNMPE LLNINGIHVF ERTVKMSSYL LAFVFGELQY KKGKTKSGVE 200
    VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH SWHIALPDFS 250
    SGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 300
    MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD 350
    GVQSVHVEVT HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL 400
    YFKRHQYGNT VGDNLWDALA EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD 450
    TLILSQKQFF VGEGVDKGRL WNVPLNTNWT GLPDLLSSEK VEIPGFAALK 500
    TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV TKFQILQDRK 550
    LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE 600
    KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK 650
    TKASQIFATH KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS 700
    LQEFKRELEG AVALIKDEKV IAELLESFKN ADIVKPQDIA FSWFYLLRND 750
    FSQDAAWAWE KANWASLEEK LGGDMSYDKF VIYPGNTFKT ADKLAEYKAF 800
    FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL 846
    Length:846
    Mass (Da):95,309
    Last modified:May 1, 2007 - v1
    Checksum:i8606417173834D40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61230 Genomic DNA. Translation: CAA43547.1.
    PIRiJN0324.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61230 Genomic DNA. Translation: CAA43547.1 .
    PIRi JN0324.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P0C2T8.

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a gene (lap) encoding a 95.3-kDa aminopeptidase from Lactococcus lactis ssp. cremoris Wg2."
      Stroman P.
      Gene 113:107-112(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Wg2.
    2. "Purification and characterization of an aminopeptidase from Lactococcus lactis subsp. cremoris Wg2."
      Tan P.S.T., Konings W.N.
      Appl. Environ. Microbiol. 56:526-532(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-34.
      Strain: Wg2.

    Entry informationi

    Entry nameiAMPN_LACLC
    AccessioniPrimary (citable) accession number: P0C2T8
    Secondary accession number(s): P37897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3