Reviewed, UniProtKB/Swiss-Prot P0C2T8 (AMPN_LACLC)

Names and origin

Protein names

Recommended name:
    Aminopeptidase N
    EC=3.4.11.2
Alternative name(s):
    Lysyl aminopeptidase
      Short name=Lys-AP
    Alanine aminopeptidase

Gene names

Name:	pepN
Synonyms:	lap

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	846 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity. Note: It may be secreted through an unknown mechanism By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 846	845	Aminopeptidase N		PRO_0000095075
Sites
	Active site	289	1	By similarity
	Active site	375	1	Proton donor Potential
	Metal binding	288	1	Zinc; catalytic By similarity
	Metal binding	292	1	Zinc; catalytic By similarity
	Metal binding	311	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C2T8-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 8606417173834D40	FASTA	846	95,309
	10 20 30 40 50 60 MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVAFHAKGL HFNKVRAFSV 70 80 90 100 110 120 DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI YPSYYEVNGE KKMLIGTQFE 130 140 150 160 170 180 SHFARQAFPS IDEPEAKATF DLSVKFDEEE GDIIVSNMPE LLNINGIHVF ERTVKMSSYL 190 200 210 220 230 240 LAFVFGELQY KKGKTKSGVE VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH 250 260 270 280 290 300 SWHIALPDFS SGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 310 320 330 340 350 360 MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD GVQSVHVEVT 370 380 390 400 410 420 HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL YFKRHQYGNT VGDNLWDALA 430 440 450 460 470 480 EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD TLILSQKQFF VGEGVDKGRL WNVPLNTNWT 490 500 510 520 530 540 GLPDLLSSEK VEIPGFAALK TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV 550 560 570 580 590 600 TKFQILQDRK LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE 610 620 630 640 650 660 KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK TKASQIFATH 670 680 690 700 710 720 KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS LQEFKRELEG AVALIKDEKV 730 740 750 760 770 780 IAELLESFKN ADIVKPQDIA FSWFYLLRND FSQDAAWAWE KANWASLEEK LGGDMSYDKF 790 800 810 820 830 840 VIYPGNTFKT ADKLAEYKAF FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL

References

[1]	"Sequence of a gene (lap) encoding a 95.3-kDa aminopeptidase from Lactococcus lactis ssp. cremoris Wg2." Stroman P. Gene 113:107-112(1992) [PubMed: 1563625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wg2.
[2]	"Purification and characterization of an aminopeptidase from Lactococcus lactis subsp. cremoris Wg2." Tan P.S.T., Konings W.N. Appl. Environ. Microbiol. 56:526-532(1990) [PubMed: 16348128] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-34. Strain: Wg2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61230 Genomic DNA. Translation: CAA43547.1.
PIR	JN0324.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.11.2. 289716.
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_LACLC

Accession

Primary (citable) accession number: P0C2T8
Secondary accession number(s): P37897

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	November 24, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)