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P0C2T8

- AMPN_LACLC

UniProt

P0C2T8 - AMPN_LACLC

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Protein

Aminopeptidase N

Gene

pepN

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover By similarity.By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201SubstrateBy similarity
Metal bindingi288 – 2881Zinc; catalyticPROSITE-ProRule annotation
Active sitei289 – 2891Proton acceptorPROSITE-ProRule annotation
Metal bindingi292 – 2921Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi311 – 3111Zinc; catalyticPROSITE-ProRule annotation
Sitei375 – 3751Transition state stabilizerBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP0C2T8.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
Synonyms:lap
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

Cytoplasm By similarity
Note: It may be secreted through an unknown mechanism.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 846845Aminopeptidase NPRO_0000095075Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni252 – 2565Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2T8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVAFHAKGL
60 70 80 90 100
HFNKVRAFSV DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI
110 120 130 140 150
YPSYYEVNGE KKMLIGTQFE SHFARQAFPS IDEPEAKATF DLSVKFDEEE
160 170 180 190 200
GDIIVSNMPE LLNINGIHVF ERTVKMSSYL LAFVFGELQY KKGKTKSGVE
210 220 230 240 250
VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH SWHIALPDFS
260 270 280 290 300
SGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT
310 320 330 340 350
MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD
360 370 380 390 400
GVQSVHVEVT HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL
410 420 430 440 450
YFKRHQYGNT VGDNLWDALA EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD
460 470 480 490 500
TLILSQKQFF VGEGVDKGRL WNVPLNTNWT GLPDLLSSEK VEIPGFAALK
510 520 530 540 550
TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV TKFQILQDRK
560 570 580 590 600
LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE
610 620 630 640 650
KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK
660 670 680 690 700
TKASQIFATH KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS
710 720 730 740 750
LQEFKRELEG AVALIKDEKV IAELLESFKN ADIVKPQDIA FSWFYLLRND
760 770 780 790 800
FSQDAAWAWE KANWASLEEK LGGDMSYDKF VIYPGNTFKT ADKLAEYKAF
810 820 830 840
FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL
Length:846
Mass (Da):95,309
Last modified:May 1, 2007 - v1
Checksum:i8606417173834D40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61230 Genomic DNA. Translation: CAA43547.1.
PIRiJN0324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61230 Genomic DNA. Translation: CAA43547.1 .
PIRi JN0324.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P0C2T8.

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of a gene (lap) encoding a 95.3-kDa aminopeptidase from Lactococcus lactis ssp. cremoris Wg2."
    Stroman P.
    Gene 113:107-112(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wg2.
  2. "Purification and characterization of an aminopeptidase from Lactococcus lactis subsp. cremoris Wg2."
    Tan P.S.T., Konings W.N.
    Appl. Environ. Microbiol. 56:526-532(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-34.
    Strain: Wg2.

Entry informationi

Entry nameiAMPN_LACLC
AccessioniPrimary (citable) accession number: P0C2T8
Secondary accession number(s): P37897
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: October 29, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3