Reviewed, UniProtKB/Swiss-Prot P0C2T6 (LDH2_LACLC)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 2
      Short name=L-LDH 2
    EC=1.1.1.27

Gene names

Name:

ldhB

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	L-lactate dehydrogenase 2		PRO_0000168355
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	123	1	NAD or substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C2T6-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 9D5114712ACB75A4	FASTA	314	34,398
	10 20 30 40 50 60 MKITSRKVVV IGTGFVGTSI AYSMINQGLV NELVLIDVNQ DKAEGEALDL LDGISWAQEN 70 80 90 100 110 120 VIVRAGNYKD CENADIVVIT AGVNQKPGQS RLDLVNTNAK IMRSIVTQVM DSGFDGIFVI 130 140 150 160 170 180 ASNPVDILTY VAWETSGLDQ SRIVGTGTTL DTTRFRKELA TKLEIDPRSV HGYIIGEHGD 190 200 210 220 230 240 SEVAVWSHTT IGGKPILEFI VKNKKIGLED LSNLSNKVKN AAYEIIDKKQ ATYYGIGMST 250 260 270 280 290 300 ARIVKAILNN EQVILPVSAY LRGEYGQEGV FTGVPSVVNQ NGVREIIELN IDAYEMKQFE 310 KSVSQLKEVI ESIK

References

[1]

"Characterization of a lactate dehydrogenase encoded by the ldhB gene of Lactococcus lactis." Gaspar P., Coelho P., Neves A.R., Shearman C.A., Gasson M.J., Soares C.M., Baptisa A.M., Ramos A., Santos H. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY236961 Genomic DNA. Translation: AAP49572.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LACLC

Accession

Primary (citable) accession number: P0C2T6
Secondary accession number(s): Q7WYP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)