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Protein

Endoglucanase D

Gene

celD

Organism
Clostridium thermocellum (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei177Nucleophile1
Active sitei4921 Publication1
Active sitei5221 Publication1
Active sitei531Proton donorPROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase D (EC:3.2.1.4)
Short name:
EGD
Alternative name(s):
Cellulase D
Endo-1,4-beta-glucanase
Gene namesi
Name:celD
OrganismiClostridium thermocellum (Ruminiclostridium thermocellum)
Taxonomic identifieri1515 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41H → A: 79% of wild-type activity. 1
Mutagenesisi143H → A: 104% of wild-type activity. 1
Mutagenesisi146H → A: 77% of wild-type activity. 1
Mutagenesisi150H → A: 2% of wild-type activity. 1
Mutagenesisi163H → A: 124% of wild-type activity. 1
Mutagenesisi173H → A: 8% of wild-type activity. 1
Mutagenesisi174D → A: 0.08% of wild-type activity. 1
Mutagenesisi177D → A: 0.2% of wild-type activity. 1
Mutagenesisi198H → S: 17% of wild-type activity. 1
Mutagenesisi222D → A: 72% of wild-type activity. 1
Mutagenesisi245H → A: 90% of wild-type activity. 1
Mutagenesisi262H → A: 19% of wild-type activity. 1
Mutagenesisi293D → A: 98% of wild-type activity. 1
Mutagenesisi337D → A: 86% of wild-type activity. 1
Mutagenesisi421H → S: 32% of wild-type activity. 1
Mutagenesisi468H → A: 9% of wild-type activity. 1
Mutagenesisi492H → S: 25% of wild-type activity. 1
Mutagenesisi493D → A: 0.8% of wild-type activity. 1
Mutagenesisi499D → A: 224% of wild-type activity. 1
Mutagenesisi503E → A: 127% of wild-type activity. 1
Mutagenesisi519D → A: 91% of wild-type activity. 1
Mutagenesisi522D → A: 1% of wild-type activity. 1
Mutagenesisi525D → A: 118% of wild-type activity. 1
Mutagenesisi531E → A: 0.3% of wild-type activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 17Add BLAST›17
ChainiPRO_000000794918 – 625Endoglucanase DAdd BLAST608

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0825.

Structurei

Secondary structure

1625
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 37Combined sources4
Beta strandi44 – 47Combined sources4
Beta strandi53 – 58Combined sources6
Beta strandi61 – 68Combined sources8
Beta strandi70 – 74Combined sources5
Turni75 – 78Combined sources4
Beta strandi79 – 85Combined sources7
Beta strandi93 – 99Combined sources7
Turni100 – 102Combined sources3
Beta strandi108 – 110Combined sources3
Turni112 – 115Combined sources4
Helixi116 – 128Combined sources13
Beta strandi135 – 139Combined sources5
Beta strandi142 – 146Combined sources5
Helixi157 – 160Combined sources4
Beta strandi175 – 177Combined sources3
Helixi182 – 198Combined sources17
Helixi200 – 203Combined sources4
Turni211 – 214Combined sources4
Beta strandi215 – 217Combined sources3
Helixi219 – 231Combined sources13
Helixi232 – 234Combined sources3
Beta strandi243 – 248Combined sources6
Helixi258 – 260Combined sources3
Beta strandi266 – 270Combined sources5
Helixi272 – 289Combined sources18
Turni290 – 292Combined sources3
Helixi294 – 313Combined sources20
Helixi336 – 350Combined sources15
Helixi353 – 363Combined sources11
Beta strandi367 – 370Combined sources4
Helixi380 – 389Combined sources10
Helixi397 – 420Combined sources24
Turni426 – 429Combined sources4
Helixi435 – 452Combined sources18
Helixi456 – 469Combined sources14
Beta strandi483 – 486Combined sources4
Helixi494 – 498Combined sources5
Helixi527 – 530Combined sources4
Helixi534 – 545Combined sources12
Turni546 – 549Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLCX-ray1.90A1-625[»]
4CJ0X-ray1.10A1-625[»]
4CJ1X-ray1.63A1-625[»]
ProteinModelPortaliP0C2S4.
SMRiP0C2S4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C2S4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini555 – 625DockerinPROSITE-ProRule annotationAdd BLAST71

Sequence similaritiesi

Contains 1 dockerin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E08. Bacteria.
ENOG410XNTA. LUCA.

Family and domain databases

CDDicd02850. E_set_Cellulase_N. 1 hit.
Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR004197. Cellulase_Ig-like.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SLTGVFPSGL IETKVSAAKI TENYQFDSRI RLNSIGFIPN HSKKATIAAN
60 70 80 90 100
CSTFYVVKED GTIVYTGTAT SMFDNDTKET VYIADFSSVN EEGTYYLAVP
110 120 130 140 150
GVGKSVNFKI AMNVYEDAFK TAMLGMYLLR CGTSVSATYN GIHYSHGPCH
160 170 180 190 200
TNDAYLDYIN GQHTKKDSTK GWHDAGDYNK YVVNAGITVG SMFLAWEHFK
210 220 230 240 250
DQLEPVALEI PEKNNSIPDF LDELKYEIDW ILTMQYPDGS GRVAHKVSTR
260 270 280 290 300
NFGGFIMPEN EHDERFFVPW SSAATADFVA MTAMAARIFR PYDPQYAEKC
310 320 330 340 350
INAAKVSYEF LKNNPANVFA NQSGFSTGEY ATVSDADDRL WAAAEMWETL
360 370 380 390 400
GDEEYLRDFE NRAAQFSKKI EADFDWDNVA NLGMFTYLLS ERPGKNPALV
410 420 430 440 450
QSIKDSLLST ADSIVRTSQN HGYGRTLGTT YYWGCNGTVV RQTMILQVAN
460 470 480 490 500
KISPNNDYVN AALDAISHVF GRNYYNRSYV TGLGINPPMN PHDRRSGADG
510 520 530 540 550
IWEPWPGYLV GGGWPGPKDW VDIQDSYQTN EIAINWNAAL IYALAGFVNY
560 570 580 590 600
NSPQNEVLYG DVNDDGKVNS TDLTLLKRYV LKAVSTLPSS KAEKNADVNR
610 620
DGRVNSSDVT ILSRYLIRVI EKLPI
Length:625
Mass (Da):69,707
Last modified:April 17, 2007 - v1
Checksum:i6BFD88E2BDF1BD8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

PIRiA25535. CZCLDM.

Cross-referencesi

Sequence databases

PIRiA25535. CZCLDM.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLCX-ray1.90A1-625[»]
4CJ0X-ray1.10A1-625[»]
4CJ1X-ray1.63A1-625[»]
ProteinModelPortaliP0C2S4.
SMRiP0C2S4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_0825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105E08. Bacteria.
ENOG410XNTA. LUCA.

Miscellaneous databases

EvolutionaryTraceiP0C2S4.

Family and domain databases

CDDicd02850. E_set_Cellulase_N. 1 hit.
Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR004197. Cellulase_Ig-like.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUND_CLOTM
AccessioniPrimary (citable) accession number: P0C2S4
Secondary accession number(s): P04954
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The sequence shown here has been extracted from PDB entry 1CLC.Curated

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.