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P0C2S1

- CELK_CLOTM

UniProt

P0C2S1 - CELK_CLOTM

Protein

Cellulose 1,4-beta-cellobiosidase

Gene

celK

Organism
Clostridium thermocellum (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.1 Publication

    Enzyme regulationi

    Inhibited by cellobiose.1 Publication

    Kineticsi

    1. KM=1.67 µM for PNP-cellobioside1 Publication

    Vmax=15.1 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Temperature dependencei

    Retains 97% of original activity when incubated for 200 hours at 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei737 – 7371By similarity
    Active sitei786 – 7861By similarity
    Active sitei795 – 7951By similarity

    GO - Molecular functioni

    1. cellulase activity Source: InterPro
    2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM4. Carbohydrate-Binding Module Family 4.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cellulose 1,4-beta-cellobiosidase (EC:3.2.1.91)
    Gene namesi
    Name:celK
    OrganismiClostridium thermocellum (Ruminiclostridium thermocellum)
    Taxonomic identifieri1515 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 895868Cellulose 1,4-beta-cellobiosidasePRO_0000045748Add
    BLAST

    Structurei

    Secondary structure

    1
    895
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 353
    Helixi38 – 403
    Beta strandi57 – 604
    Beta strandi64 – 729
    Beta strandi80 – 8910
    Helixi93 – 953
    Beta strandi96 – 10510
    Beta strandi110 – 12112
    Beta strandi123 – 13311
    Beta strandi138 – 1425
    Beta strandi147 – 1504
    Beta strandi153 – 16210
    Beta strandi168 – 1769
    Helixi179 – 1813
    Beta strandi184 – 19714

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3P6BX-ray2.00A/B27-210[»]
    ProteinModelPortaliP0C2S1.
    SMRiP0C2S1. Positions 208-813, 831-895.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C2S1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 199160CBM-cenCAdd
    BLAST
    Domaini834 – 85421Dockerin 1Add
    BLAST
    Domaini866 – 88621Dockerin 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni199 – 24042LinkerAdd
    BLAST
    Regioni241 – 815575CatalyticAdd
    BLAST

    Sequence similaritiesi

    Contains 2 dockerin domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    2.60.120.260. 1 hit.
    2.60.40.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR003305. CenC_carb-bd.
    IPR018242. Dockerin_1.
    IPR008979. Galactose-bd-like.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR004197. Glyco_hydro_9_Ig-like.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF02018. CBM_4_9. 1 hit.
    PF02927. CelD_N. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF63446. SSF63446. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C2S1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSSKLPDYKN DLLYERTFDE    50
    GLCFPWHTCE DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH 100
    RGITLEQGHT YTVRFTIWSD KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT 150
    PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE LAAGTPYYVY LDDVSLYDPR 200
    FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK WQLLNSANQV 250
    VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP 300
    FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGIEPN 350
    KGDTNVPTWP QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT 400
    LMNMYERAKI RGIANQGAYK DGGMNIPERN NGYPDILDEA RWEIEFFKKM 450
    QVTEKEDPSI AGMVHHKIHD FRWTALGMLP HEDPQPRYLR PVSTAATLNF 500
    AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY AEYTPGSGGP 550
    GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN 600
    GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPATDIQ KARNNIAKAA 650
    DRWLENIEEQ GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN 700
    SKYLDGMQDG MSYLLGRNGL DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP 750
    APPPGIIAGG PNSRFEDPTI TAAVKKDTPP QKCYIDHTDS WSTNEITVNW 800
    NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK VNSTDAVALK 850
    RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK 895
    Length:895
    Mass (Da):100,712
    Last modified:April 17, 2007 - v1
    Checksum:i5DB1FD84A6750CCE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039030 Genomic DNA. Translation: AAC06139.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039030 Genomic DNA. Translation: AAC06139.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3P6B X-ray 2.00 A/B 27-210 [» ]
    ProteinModelPortali P0C2S1.
    SMRi P0C2S1. Positions 208-813, 831-895.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM4. Carbohydrate-Binding Module Family 4.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0C2S1.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    2.60.120.260. 1 hit.
    2.60.40.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR003305. CenC_carb-bd.
    IPR018242. Dockerin_1.
    IPR008979. Galactose-bd-like.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR004197. Glyco_hydro_9_Ig-like.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF02018. CBM_4_9. 1 hit.
    PF02927. CelD_N. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF63446. SSF63446. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination."
      Kataeva I., Li X.L., Chen H., Choi S.-K., Ljungdahl L.G.
      J. Bacteriol. 181:5288-5295(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 283-287 AND 326-337, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
      Strain: JW20.
    2. "Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of trucated polypeptides."
      Choi S.K., Ljungdahl L.G.
      Biochemistry 35:4897-4905(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-45, SUBCELLULAR LOCATION.
      Strain: JW20.

    Entry informationi

    Entry nameiCELK_CLOTM
    AccessioniPrimary (citable) accession number: P0C2S1
    Secondary accession number(s): O68438, Q10748, Q4CGG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3