Cellulose 1,4-beta-cellobiosidase precursor

Preferred order of sections

Names and origin

Protein names

Recommended name:
Cellulose 1,4-beta-cellobiosidase
EC=3.2.1.91

Gene names

Name:

celK

Organism

Clostridium thermocellum

Taxonomic identifier

1515 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	895 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. Ref.1
Enzyme regulation	Inhibited by cellobiose. Ref.1
Subcellular location	Secreted Ref.1 Ref.2.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM-cenC (cenC-type cellulose-binding) domain. Contains 2 dockerin domains.
Biophysicochemical properties	Kinetic parameters: K_M=1.67 µM for PNP-cellobioside Ref.1 V_max=15.1 µmol/min/mg enzyme pH dependence: Optimum pH is 6.0. Temperature dependence: Retains 97% of original activity when incubated for 200 hours at 60 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	extracellular space Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	cellulase activity Inferred from electronic annotation. Source: InterPro cellulose 1,4-beta-cellobiosidase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Ref.2

Chain

28 – 895

868

Cellulose 1,4-beta-cellobiosidase

PRO_0000045748

Regions

Domain

40 – 199

160

CBM-cenC

Domain

834 – 854

21

Dockerin 1

Domain

866 – 886

21

Dockerin 2

Region

199 – 240

42

Linker

Region

241 – 815

575

Catalytic

Sites

Active site

737

1

By similarity

Active site

786

1

By similarity

Active site

795

1

By similarity

Secondary structure

1

.

895

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0C2S1 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 5DB1FD84A6750CCE
Show »

FASTA

895

100,712

        10         20         30         40         50         60 
MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSSKLPDYKN DLLYERTFDE GLCFPWHTCE 

        70         80         90        100        110        120 
DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH RGITLEQGHT YTVRFTIWSD 

       130        140        150        160        170        180 
KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE 

       190        200        210        220        230        240 
LAAGTPYYVY LDDVSLYDPR FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK 

       250        260        270        280        290        300 
WQLLNSANQV VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP 

       310        320        330        340        350        360 
FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGIEPN KGDTNVPTWP 

       370        380        390        400        410        420 
QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT LMNMYERAKI RGIANQGAYK 

       430        440        450        460        470        480 
DGGMNIPERN NGYPDILDEA RWEIEFFKKM QVTEKEDPSI AGMVHHKIHD FRWTALGMLP 

       490        500        510        520        530        540 
HEDPQPRYLR PVSTAATLNF AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY 

       550        560        570        580        590        600 
AEYTPGSGGP GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN 

       610        620        630        640        650        660 
GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPATDIQ KARNNIAKAA DRWLENIEEQ 

       670        680        690        700        710        720 
GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN SKYLDGMQDG MSYLLGRNGL 

       730        740        750        760        770        780 
DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP APPPGIIAGG PNSRFEDPTI TAAVKKDTPP 

       790        800        810        820        830        840 
QKCYIDHTDS WSTNEITVNW NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK 

       850        860        870        880        890 
VNSTDAVALK RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK

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References

[1]	"Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination." Kataeva I., Li X.L., Chen H., Choi S.-K., Ljungdahl L.G. J. Bacteriol. 181:5288-5295(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 283-287 AND 326-337, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION. Strain: JW20.
[2]	"Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of trucated polypeptides." Choi S.K., Ljungdahl L.G. Biochemistry 35:4897-4905(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-45, SUBCELLULAR LOCATION. Strain: JW20.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF039030 Genomic DNA. Translation: AAC06139.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P6B	X-ray	2.00	A/B	27-210	[»]

ProteinModelPortal

P0C2S1.

SMR

P0C2S1. Positions 208-813, 831-895.

ModBase

Search...

Protein family/group databases

CAZy

CBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.10. 1 hit.

InterPro

IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]

Pfam

PF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]

SUPFAM

SSF63446. Cellulos_enz_dockerin_1. 1 hit.
SSF49785. Gal_bind_like. 1 hit.
SSF48208. Glyco_trans_6hp. 1 hit.
SSF81296. Ig_E-set. 1 hit.

PROSITE

PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C2S1.

Entry information

Entry name

CELK_CLOTM

Accession

Primary (citable) accession number: P0C2S1
Secondary accession number(s): O68438, Q10748, Q4CGG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	April 3, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families