Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0C2S1

- CELK_CLOTM

UniProt

P0C2S1 - CELK_CLOTM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cellulose 1,4-beta-cellobiosidase

Gene

celK

Organism
Clostridium thermocellum (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.1 Publication

Enzyme regulationi

Inhibited by cellobiose.1 Publication

Kineticsi

  1. KM=1.67 µM for PNP-cellobioside1 Publication

Vmax=15.1 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Temperature dependencei

Retains 97% of original activity when incubated for 200 hours at 60 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei737 – 7371By similarity
Active sitei786 – 7861By similarity
Active sitei795 – 7951By similarity

GO - Molecular functioni

  1. cellulase activity Source: InterPro
  2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Cellulose 1,4-beta-cellobiosidase (EC:3.2.1.91)
Gene namesi
Name:celK
OrganismiClostridium thermocellum (Ruminiclostridium thermocellum)
Taxonomic identifieri1515 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 895868Cellulose 1,4-beta-cellobiosidasePRO_0000045748Add
BLAST

Structurei

Secondary structure

1
895
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 353Combined sources
Helixi38 – 403Combined sources
Beta strandi57 – 604Combined sources
Beta strandi64 – 729Combined sources
Beta strandi80 – 8910Combined sources
Helixi93 – 953Combined sources
Beta strandi96 – 10510Combined sources
Beta strandi110 – 12112Combined sources
Beta strandi123 – 13311Combined sources
Beta strandi138 – 1425Combined sources
Beta strandi147 – 1504Combined sources
Beta strandi153 – 16210Combined sources
Beta strandi168 – 1769Combined sources
Helixi179 – 1813Combined sources
Beta strandi184 – 19714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P6BX-ray2.00A/B27-210[»]
ProteinModelPortaliP0C2S1.
SMRiP0C2S1. Positions 208-813, 831-895.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C2S1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 199160CBM-cenCAdd
BLAST
Domaini834 – 85421Dockerin 1Add
BLAST
Domaini866 – 88621Dockerin 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 24042LinkerAdd
BLAST
Regioni241 – 815575CatalyticAdd
BLAST

Sequence similaritiesi

Contains 2 dockerin domains.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2S1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSSKLPDYKN DLLYERTFDE
60 70 80 90 100
GLCFPWHTCE DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH
110 120 130 140 150
RGITLEQGHT YTVRFTIWSD KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT
160 170 180 190 200
PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE LAAGTPYYVY LDDVSLYDPR
210 220 230 240 250
FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK WQLLNSANQV
260 270 280 290 300
VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP
310 320 330 340 350
FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGIEPN
360 370 380 390 400
KGDTNVPTWP QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT
410 420 430 440 450
LMNMYERAKI RGIANQGAYK DGGMNIPERN NGYPDILDEA RWEIEFFKKM
460 470 480 490 500
QVTEKEDPSI AGMVHHKIHD FRWTALGMLP HEDPQPRYLR PVSTAATLNF
510 520 530 540 550
AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY AEYTPGSGGP
560 570 580 590 600
GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN
610 620 630 640 650
GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPATDIQ KARNNIAKAA
660 670 680 690 700
DRWLENIEEQ GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN
710 720 730 740 750
SKYLDGMQDG MSYLLGRNGL DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP
760 770 780 790 800
APPPGIIAGG PNSRFEDPTI TAAVKKDTPP QKCYIDHTDS WSTNEITVNW
810 820 830 840 850
NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK VNSTDAVALK
860 870 880 890
RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK
Length:895
Mass (Da):100,712
Last modified:April 17, 2007 - v1
Checksum:i5DB1FD84A6750CCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039030 Genomic DNA. Translation: AAC06139.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039030 Genomic DNA. Translation: AAC06139.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3P6B X-ray 2.00 A/B 27-210 [» ]
ProteinModelPortali P0C2S1.
SMRi P0C2S1. Positions 208-813, 831-895.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P0C2S1.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
Pfami PF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination."
    Kataeva I., Li X.L., Chen H., Choi S.-K., Ljungdahl L.G.
    J. Bacteriol. 181:5288-5295(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 283-287 AND 326-337, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: JW20.
  2. "Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of trucated polypeptides."
    Choi S.K., Ljungdahl L.G.
    Biochemistry 35:4897-4905(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-45, SUBCELLULAR LOCATION.
    Strain: JW20.

Entry informationi

Entry nameiCELK_CLOTM
AccessioniPrimary (citable) accession number: P0C2S1
Secondary accession number(s): O68438, Q10748, Q4CGG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: November 26, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3