P0C2J9 (TACY_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pneumolysin

Alternative name(s):
Thiol-activated cytolysin

Gene names

Name:	ply
Ordered Locus Names:	SP_1923

Organism

Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]

Taxonomic identifier

170187 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation By similarity.
Subunit structure	Homooligomeric pore complex; when inserted in the host membrane By similarity.
Subcellular location	Secreted By similarity. Host cell membrane; Multi-pass membrane protein By similarity. Note: Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands By similarity.
Sequence similarities	Belongs to the thiol-activated cytolysin family.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Host cell membrane Host membrane Membrane Secreted
Domain	Transmembrane Transmembrane beta strand
Ligand	Lipid-binding
Molecular function	Toxin
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cholesterol binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 471	470	Pneumolysin		PRO_0000204251
Sites
	Site	428	1	Binding to cholesterol By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C2J9 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 986A146548E0F738	FASTA	471	52,898
	10 20 30 40 50 60 MANKAVNDFI LAMNYDKKKL LTHQGESIEN RFIKEGNQLP DEFVVIERKK RSLSTNTSDI 70 80 90 100 110 120 SVTATNDSRL YPGALLVVDE TLLENNPTLL AVDRAPMTYS IDLPGLASSD SFLQVEDPSN 130 140 150 160 170 180 SSVRGAVNDL LAKWHQDYGQ VNNVPARMQY EKITAHSMEQ LKVKFGSDFE KTGNSLDIDF 190 200 210 220 230 240 NSVHSGEKQI QIVNFKQIYY TVSVDAVKNP GDVFQDTVTV EDLKQRGISA ERPLVYISSV 250 260 270 280 290 300 AYGRQVYLKL ETTSKSDEVE AAFEALIKGV KVAPQTEWKQ ILDNTEVKAV ILGGDPSSGA 310 320 330 340 350 360 RVVTGKVDMV EDLIQEGSRF TADHPGLPIS YTTSFLRDNV VATFQNSTDY VETKVTAYRN 370 380 390 400 410 420 GDLLLDHSGA YVAQYYITWN ELSYDHQGKE VLTPKAWDRN GQDLTAHFTT SIPLKGNVRN 430 440 450 460 470 LSVKIRECTG LAWEWWRTVY EKTDLPLVRK RTISIWGTTL YPQVEDKVEN D

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK75991.1.
PIR	A28568. A99089.
RefSeq	NP_346351.1. NC_003028.3.
3D structure databases
ProteinModelPortal	P0C2J9.
SMR	P0C2J9. Positions 2-469.
ModBase	Search...
Protein-protein interaction databases
STRING	170187.SP_1923.
Protein family/group databases
TCDB	1.C.12.1.5. thiol-activated cholesterol-dependent cytolysin (CDC) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK75991; AAK75991; SP_1923.
GeneID	931915.
KEGG	spn:SP_1923.
PATRIC	19708367. VBIStrPne105772_2005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG04845.
HOGENOM	HOG000056095.
KO	K11031.
OMA	VAYGRTI.
ProtClustDB	CLSK877006.
Family and domain databases
Gene3D	3.90.840.10. 4 hits.
InterPro	IPR001869. Thiol_cytolysin. [Graphical view]
Pfam	PF01289. Thiol_cytolysin. 1 hit. [Graphical view]
PRINTS	PR01400. TACYTOLYSIN.
SUPFAM	SSF56978. Thiol_cytolysin. 1 hit.
PROSITE	PS00481. THIOL_CYTOLYSINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5117.

Entry information

Entry name

TACY_STRPN

Accession

Primary (citable) accession number: P0C2J9
Secondary accession number(s): P11990

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program