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P0C2J9 (TACY_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pneumolysin
Alternative name(s):
Thiol-activated cytolysin
Gene names
Name:ply
Ordered Locus Names:SP_1923
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation By similarity.

Subunit structure

Homooligomeric pore complex; when inserted in the host membrane By similarity.

Subcellular location

Secreted By similarity. Host cell membrane; Multi-pass membrane protein By similarity. Note: Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands By similarity.

Sequence similarities

Belongs to the thiol-activated cytolysin family.

Ontologies

Keywords
   Biological processCytolysis
Hemolysis
   Cellular componentHost cell membrane
Host membrane
Membrane
Secreted
   DomainTransmembrane
Transmembrane beta strand
   LigandLipid-binding
   Molecular functionToxin
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processhemolysis in other organism

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncholesterol binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 471470Pneumolysin
PRO_0000204251

Sites

Site4281Binding to cholesterol By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C2J9 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 986A146548E0F738

FASTA47152,898
        10         20         30         40         50         60 
MANKAVNDFI LAMNYDKKKL LTHQGESIEN RFIKEGNQLP DEFVVIERKK RSLSTNTSDI 

        70         80         90        100        110        120 
SVTATNDSRL YPGALLVVDE TLLENNPTLL AVDRAPMTYS IDLPGLASSD SFLQVEDPSN 

       130        140        150        160        170        180 
SSVRGAVNDL LAKWHQDYGQ VNNVPARMQY EKITAHSMEQ LKVKFGSDFE KTGNSLDIDF 

       190        200        210        220        230        240 
NSVHSGEKQI QIVNFKQIYY TVSVDAVKNP GDVFQDTVTV EDLKQRGISA ERPLVYISSV 

       250        260        270        280        290        300 
AYGRQVYLKL ETTSKSDEVE AAFEALIKGV KVAPQTEWKQ ILDNTEVKAV ILGGDPSSGA 

       310        320        330        340        350        360 
RVVTGKVDMV EDLIQEGSRF TADHPGLPIS YTTSFLRDNV VATFQNSTDY VETKVTAYRN 

       370        380        390        400        410        420 
GDLLLDHSGA YVAQYYITWN ELSYDHQGKE VLTPKAWDRN GQDLTAHFTT SIPLKGNVRN 

       430        440        450        460        470 
LSVKIRECTG LAWEWWRTVY EKTDLPLVRK RTISIWGTTL YPQVEDKVEN D 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005672 Genomic DNA. Translation: AAK75991.1.
PIRA28568.
A99089.
RefSeqNP_346351.1. NC_003028.3.

3D structure databases

ProteinModelPortalP0C2J9.
SMRP0C2J9. Positions 2-469.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING170187.SP_1923.

Chemistry

ChEMBLCHEMBL5117.

Protein family/group databases

TCDB1.C.12.1.5. thiol-activated cholesterol-dependent cytolysin (cdc) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75991; AAK75991; SP_1923.
GeneID931915.
KEGGspn:SP_1923.
PATRIC19708367. VBIStrPne105772_2005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG04845.
HOGENOMHOG000056095.
KOK11031.
OMAVAYGRTI.
OrthoDBEOG6S7XT6.
ProtClustDBCLSK877006.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1953-MONOMER.

Family and domain databases

Gene3D3.90.840.10. 4 hits.
InterProIPR001869. Thiol_cytolysin.
[Graphical view]
PfamPF01289. Thiol_cytolysin. 1 hit.
[Graphical view]
PRINTSPR01400. TACYTOLYSIN.
SUPFAMSSF56978. SSF56978. 1 hit.
PROSITEPS00481. THIOL_CYTOLYSINS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTACY_STRPN
AccessionPrimary (citable) accession number: P0C2J9
Secondary accession number(s): P11990
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families