P0C2H8 (RL31A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L31-A

Alternative name(s):
L34
YL28

Gene names

Name:	RPL31A
Synonyms:	RPL34, RPL34A
Ordered Locus Names:	YDL075W
ORF Names:	D2478

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.9
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 genes for L31 in yeast.
Sequence similarities	Belongs to the ribosomal protein L31e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from direct assay PubMed 18782943. Source: SGD regulation of translational fidelity Inferred from mutant phenotype PubMed 18829863. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay PubMed 18782943. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay PubMed 18782943. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4 Ref.5
	Chain	2 – 113	112	60S ribosomal protein L31-A		PRO_0000153788
Amino acid modifications
	Modified residue	100	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P0C2H8 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 351B5ADD15DB3B5D	FASTA	113	12,953
	10 20 30 40 50 60 MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW 70 80 90 100 110 KRGVKGVEYR LRLRISRKRN EEEDAKNPLF SYVEPVLVAS AKGLQTVVVE EDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of a gene encoding yeast ribosomal protein L34." Schaap P.J., Molenaar C.M.T., Mager W.H., Planta R.J. Curr. Genet. 9:47-52(1984) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Carlsbergensis.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PROTEIN SEQUENCE OF 2-51.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, MASS SPECTROMETRY.
[7]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 6-83, ELECTRON MICROSCOPY.
[8]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
[9]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01441 Genomic DNA. Translation: CAA25679.1.
Z74123 Genomic DNA. Translation: CAA98641.1.
BK006938 Genomic DNA. Translation: DAA11784.1.

PIR

R5BY1E. S67611.

RefSeq

NP_010208.1. NM_001180134.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	W	6-83	[»]
1S1I	electron microscopy	11.70	W	2-113	[»]
2WW9	electron microscopy	8.60	M	1-113	[»]
2WWA	electron microscopy	8.90	M	1-113	[»]
3IZS	electron microscopy	-	g	1-113	[»]
3O58	X-ray	4.00	a	1-113	[»]
3O5H	X-ray	4.00	a	1-113	[»]
3U5E	X-ray	3.00	d	1-113	[»]
3U5I	X-ray	3.00	d	1-113	[»]
4B6A	electron microscopy	8.10	d	1-113	[»]

ProteinModelPortal

P0C2H8.

SMR

P0C2H8. Positions 4-112.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5135N.

Proteomic databases

PeptideAtlas

P0C2H8.

PRIDE

P0C2H8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDL075W; YDL075W; YDL075W.

GeneID

851484.

KEGG

sce:YDL075W.

Organism-specific databases

SGD

S000002233. RPL31A.

Phylogenomic databases

GeneTree

ENSGT00390000005200.

HOGENOM

HOG000216660.

KO

K02910.

OMA

NECEANE.

OrthoDB

EOG4H75N6.

Gene expression databases

ArrayExpress

P0C2H8.

Genevestigator

P0C2H8.

GermOnline

YDL075W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.10.440.10. 1 hit.

InterPro

IPR000054. Ribosomal_L31e.
IPR020052. Ribosomal_L31e_CS.
IPR023621. Ribosomal_L31e_dom.
[Graphical view]

PANTHER

PTHR10956. PTHR10956. 1 hit.

Pfam

PF01198. Ribosomal_L31e. 1 hit.
[Graphical view]

ProDom

PD006030. Ribosomal_L31e. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF54575. Ribosomal_L31e. 1 hit.

PROSITE

PS01144. RIBOSOMAL_L31E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0C2H8.

NextBio

968804.

Entry information

Entry name

RL31A_YEAST

Accession

Primary (citable) accession number: P0C2H8
Secondary accession number(s): D6VRS4, P04649, Q3E7B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program