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Protein

60S ribosomal protein L27-B

Gene

RPL27B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 22900 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL27 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29998-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L27-B1 Publication
Alternative name(s):
Large ribosomal subunit protein eL27-B1 Publication
Gene namesi
Name:RPL27B1 Publication
Synonyms:RPL27
Ordered Locus Names:YDR471W
ORF Names:D8035.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR471W.
SGDiS000002879. RPL27B.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: GO_Central
  • preribosome, large subunit precursor Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002789672 – 13660S ribosomal protein L27-BAdd BLAST135

Proteomic databases

MaxQBiP0C2H7.
PRIDEiP0C2H7.
TopDownProteomicsiP0C2H7.

PTM databases

iPTMnetiP0C2H7.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi32524. 165 interactors.
IntActiP0C2H7. 3 interactors.
MINTiMINT-8285603.

Structurei

3D structure databases

ProteinModelPortaliP0C2H7.
SMRiP0C2H7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000010721.
HOGENOMiHOG000210138.
InParanoidiP0C2H7.
KOiK02901.
OMAiFVKYINV.
OrthoDBiEOG092C5ISN.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiView protein in InterPro
IPR014722. Rib_L2_dom2.
IPR001141. Ribosomal_L27e.
IPR018262. Ribosomal_L27e_CS.
IPR008991. Translation_prot_SH3-like.
PANTHERiPTHR10497. PTHR10497. 1 hit.
PfamiView protein in Pfam
PF01777. Ribosomal_L27e. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009396. Ribosomal_L27e. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiView protein in PROSITE
PS01107. RIBOSOMAL_L27E. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2H7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP
60 70 80 90 100
SKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDVE AFKSVVSTET
110 120 130
FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF
Length:136
Mass (Da):15,505
Last modified:March 6, 2007 - v1
Checksum:i427D55EC9AB4A2E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64935.1.
BK006938 Genomic DNA. Translation: DAA12305.1.
PIRiS69638.
RefSeqiNP_010759.1. NM_001180779.1.

Genome annotation databases

EnsemblFungiiYDR471W; YDR471W; YDR471W.
GeneIDi852082.
KEGGisce:YDR471W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64935.1.
BK006938 Genomic DNA. Translation: DAA12305.1.
PIRiS69638.
RefSeqiNP_010759.1. NM_001180779.1.

3D structure databases

ProteinModelPortaliP0C2H7.
SMRiP0C2H7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32524. 165 interactors.
IntActiP0C2H7. 3 interactors.
MINTiMINT-8285603.

PTM databases

iPTMnetiP0C2H7.

Proteomic databases

MaxQBiP0C2H7.
PRIDEiP0C2H7.
TopDownProteomicsiP0C2H7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR471W; YDR471W; YDR471W.
GeneIDi852082.
KEGGisce:YDR471W.

Organism-specific databases

EuPathDBiFungiDB:YDR471W.
SGDiS000002879. RPL27B.

Phylogenomic databases

GeneTreeiENSGT00390000010721.
HOGENOMiHOG000210138.
InParanoidiP0C2H7.
KOiK02901.
OMAiFVKYINV.
OrthoDBiEOG092C5ISN.

Enzyme and pathway databases

BioCyciYEAST:G3O-29998-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiPR:P0C2H7.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiView protein in InterPro
IPR014722. Rib_L2_dom2.
IPR001141. Ribosomal_L27e.
IPR018262. Ribosomal_L27e_CS.
IPR008991. Translation_prot_SH3-like.
PANTHERiPTHR10497. PTHR10497. 1 hit.
PfamiView protein in Pfam
PF01777. Ribosomal_L27e. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009396. Ribosomal_L27e. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiView protein in PROSITE
PS01107. RIBOSOMAL_L27E. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRL27B_YEAST
AccessioniPrimary (citable) accession number: P0C2H7
Secondary accession number(s): D6VT95, P38706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: June 7, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.