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Protein

60S ribosomal protein L27-A

Gene

RPL27A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 7380 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL27 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-31073-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L27-A1 Publication
Alternative name(s):
Large ribosomal subunit protein eL27-A1 Publication
Gene namesi
Name:RPL27A1 Publication
Synonyms:RPL27
Ordered Locus Names:YHR010W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR010W.
SGDiS000001052. RPL27A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001260922 – 13660S ribosomal protein L27-AAdd BLAST135

Proteomic databases

MaxQBiP0C2H6.
PRIDEiP0C2H6.
TopDownProteomicsiP0C2H6.

PTM databases

iPTMnetiP0C2H6.

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi36437. 84 interactors.
IntActiP0C2H6. 4 interactors.
MINTiMINT-8285600.
STRINGi4932.YHR010W.

Structurei

Secondary structure

1136
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi9 – 13Combined sources5
Beta strandi15 – 18Combined sources4
Beta strandi22 – 29Combined sources8
Beta strandi34 – 36Combined sources3
Beta strandi40 – 48Combined sources9
Beta strandi55 – 57Combined sources3
Helixi61 – 65Combined sources5
Beta strandi69 – 76Combined sources8
Helixi77 – 79Combined sources3
Beta strandi80 – 86Combined sources7
Helixi90 – 92Combined sources3
Turni93 – 95Combined sources3
Turni98 – 100Combined sources3
Beta strandi101 – 103Combined sources3
Helixi104 – 122Combined sources19
Turni123 – 127Combined sources5
Helixi128 – 131Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10671-136[»]
3J6Yelectron microscopy6.10671-136[»]
3J77electron microscopy6.20771-136[»]
3J78electron microscopy6.30771-136[»]
3JCTelectron microscopy3.08Z1-136[»]
4U3MX-ray3.00N7/n72-136[»]
4U3NX-ray3.20N7/n72-136[»]
4U3UX-ray2.90N7/n72-136[»]
4U4NX-ray3.10N7/n72-136[»]
4U4OX-ray3.60N7/n72-136[»]
4U4QX-ray3.00N7/n72-136[»]
4U4RX-ray2.80N7/n72-136[»]
4U4UX-ray3.00N7/n72-136[»]
4U4YX-ray3.20N7/n72-136[»]
4U4ZX-ray3.10N7/n72-136[»]
4U50X-ray3.20N7/n72-136[»]
4U51X-ray3.20N7/n72-136[»]
4U52X-ray3.00N7/n72-136[»]
4U53X-ray3.30N7/n72-136[»]
4U55X-ray3.20N7/n72-136[»]
4U56X-ray3.45N7/n72-136[»]
4U6FX-ray3.10N7/n72-136[»]
4V6Ielectron microscopy8.80Ba1-136[»]
4V7Felectron microscopy8.70Y1-136[»]
4V88X-ray3.00BZ/DZ1-136[»]
4V8Telectron microscopy8.10Z1-136[»]
4V8Yelectron microscopy4.30BZ2-136[»]
4V8Zelectron microscopy6.60BZ2-136[»]
4V91electron microscopy3.70Z1-136[»]
5APNelectron microscopy3.91Z1-136[»]
5APOelectron microscopy3.41Z1-136[»]
5DATX-ray3.15N7/n72-136[»]
5DC3X-ray3.25N7/n72-136[»]
5DGEX-ray3.45N7/n72-136[»]
5DGFX-ray3.30N7/n72-136[»]
5DGVX-ray3.10N7/n72-136[»]
5FCIX-ray3.40N7/n72-136[»]
5FCJX-ray3.10N7/n72-136[»]
5FL8electron microscopy9.50Z1-136[»]
5GAKelectron microscopy3.88b1-136[»]
5H4Pelectron microscopy3.07Z1-136[»]
5I4LX-ray3.10N7/n72-136[»]
5JCSelectron microscopy9.50Z1-136[»]
5JUOelectron microscopy4.00EA1-136[»]
5JUPelectron microscopy3.50EA1-136[»]
5JUSelectron microscopy4.20EA1-136[»]
5JUTelectron microscopy4.00EA1-136[»]
5JUUelectron microscopy4.00EA1-136[»]
5LYBX-ray3.25N7/n72-136[»]
5M1Jelectron microscopy3.30Z52-136[»]
5MC6electron microscopy3.80AN1-136[»]
5T62electron microscopy3.30m1-136[»]
5T6Relectron microscopy4.50m1-136[»]
5TGAX-ray3.30N7/n72-136[»]
5TGMX-ray3.50N7/n72-136[»]
ProteinModelPortaliP0C2H6.
SMRiP0C2H6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000210138.
InParanoidiP0C2H6.
KOiK02901.
OMAiRYPRKVH.
OrthoDBiEOG092C5ISN.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiView protein in InterPro
IPR014722. Rib_L2_dom2.
IPR001141. Ribosomal_L27e.
IPR018262. Ribosomal_L27e_CS.
IPR008991. Translation_prot_SH3-like.
PANTHERiPTHR10497. PTHR10497. 1 hit.
PfamiView protein in Pfam
PF01777. Ribosomal_L27e. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009396. Ribosomal_L27e. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiView protein in PROSITE
PS01107. RIBOSOMAL_L27E. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP
60 70 80 90 100
LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDVE AFKSVVSTET
110 120 130
FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF
Length:136
Mass (Da):15,531
Last modified:March 6, 2007 - v1
Checksum:iF3924B039AA2D85D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10400 Genomic DNA. Translation: AAB68944.1.
BK006934 Genomic DNA. Translation: DAA06699.1.
PIRiS46790.
RefSeqiNP_011874.1. NM_001179140.1.

Genome annotation databases

EnsemblFungiiYHR010W; YHR010W; YHR010W.
GeneIDi856401.
KEGGisce:YHR010W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRL27A_YEAST
AccessioniPrimary (citable) accession number: P0C2H6
Secondary accession number(s): D3DKV5, P38706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: July 5, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names