Reviewed, UniProtKB/Swiss-Prot P0C2H6 (RL27A_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L27-A

Gene names

Name:	RPL27A
Synonyms:	RPL27
Ordered Locus Names:	YHR010W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.2
Subcellular location	Cytoplasm.
Miscellaneous	Present with 7380 molecules/cell in log phase SD medium. Ref.5 There are 2 genes for L27 in yeast.
Sequence similarities	Belongs to the ribosomal protein L27e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Ref.2 Traceable author statement. Source: SGD
Cellular component	cytosolic large ribosomal subunit Ref.2 Traceable author statement. Source: SGD
Molecular function	structural constituent of ribosome Ref.2 Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 136	135	60S ribosomal protein L27-A		PRO_0000126092

Sequences

	Sequence		Length	Mass (Da)
	P0C2H6-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: F3924B039AA2D85D	FASTA	136	15,531
	10 20 30 40 50 60 MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK 70 80 90 100 110 120 KVAKRTKIKP FIKVVNYNHL LPTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE 130 RHQAGKNQWF FSKLRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[3]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10400 Genomic DNA. Translation: AAB68944.1.
PIR	S46790.
RefSeq	NP_011874.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P0C2H6.
Proteomic databases
PRIDE	P0C2H6.
Genome annotation databases
Ensembl	YHR010W; YHR010W; YHR010W; Saccharomyces cerevisiae. [Genome view]
GeneID	856401.
KEGG	sce:YHR010W.
Organism-specific databases
CYGD	YHR010w.
SGD	S000001052. RPL27A.
Phylogenomic databases
HOGENOM	P0C2H6.
OMA	SKFEERY
OrthoDB	EOG9JM965
Gene expression databases
ArrayExpress	P0C2H6.
Genevestigator	P0C2H6.
GermOnline	YHR010W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001141. Ribosomal_L27e. IPR018262. Ribosomal_L27e_CS. [Graphical view]
PANTHER	PTHR10497. Ribosomal_L27e. 1 hit.
Pfam	PF01777. Ribosomal_L27e. 1 hit. [Graphical view]
ProDom	PD009396. Ribosomal_L27e. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01107. RIBOSOMAL_L27E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981934.

Entry information

Entry name

RL27A_YEAST

Accession

Primary (citable) accession number: P0C2H6
Secondary accession number(s): P38706

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	November 24, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)