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Protein

60S ribosomal protein L27-A

Gene

RPL27A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-31073-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L27-A
Gene namesi
Name:RPL27A
Synonyms:RPL27
Ordered Locus Names:YHR010W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

SGDiS000001052. RPL27A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 13613560S ribosomal protein L27-APRO_0000126092Add
BLAST

Proteomic databases

MaxQBiP0C2H6.

Expressioni

Gene expression databases

GenevestigatoriP0C2H6.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi36437. 93 interactions.
IntActiP0C2H6. 4 interactions.
MINTiMINT-8285600.
STRINGi4932.YHR010W.

Structurei

Secondary structure

1
136
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83Combined sources
Beta strandi9 – 135Combined sources
Beta strandi15 – 184Combined sources
Beta strandi22 – 298Combined sources
Beta strandi34 – 363Combined sources
Beta strandi40 – 489Combined sources
Beta strandi55 – 573Combined sources
Helixi61 – 655Combined sources
Beta strandi69 – 768Combined sources
Helixi77 – 793Combined sources
Beta strandi80 – 867Combined sources
Helixi90 – 923Combined sources
Turni93 – 953Combined sources
Turni98 – 1003Combined sources
Beta strandi101 – 1033Combined sources
Helixi104 – 12219Combined sources
Turni123 – 1275Combined sources
Helixi128 – 1314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW8electron microscopy6.10a1-136[»]
1VWUelectron microscopy6.10a1-136[»]
1VXVelectron microscopy6.20a1-136[»]
1VXYelectron microscopy6.30a1-136[»]
3IZSelectron microscopy-a1-136[»]
3J65electron microscopy8.70Y1-136[»]
3U5EX-ray3.00Z1-136[»]
3U5IX-ray3.00Z1-136[»]
4B6Aelectron microscopy8.10Z1-136[»]
4BYNelectron microscopy4.30Z2-136[»]
4BYUelectron microscopy6.60Z2-136[»]
4CUWelectron microscopy3.70Z1-136[»]
4UJHX-ray3.20M2-136[»]
4UJJX-ray3.20R2-136[»]
4UJMX-ray3.00M2-136[»]
4UJOX-ray3.00R2-136[»]
4UJRX-ray2.80M2-136[»]
4UJTX-ray2.80R2-136[»]
4UJWX-ray3.10M2-136[»]
4UJYX-ray3.10Q2-136[»]
4UK1X-ray3.00M2-136[»]
4UK3X-ray3.00R2-136[»]
4UK6X-ray3.20M2-136[»]
4UK8X-ray3.20R2-136[»]
4UKBX-ray2.90M2-136[»]
4UKEX-ray2.90R2-136[»]
4UKHX-ray3.45M2-136[»]
4UKJX-ray3.45Q2-136[»]
4UKMX-ray3.20M2-136[»]
4UKOX-ray3.20Q2-136[»]
4UKRX-ray3.30M2-136[»]
4UKTX-ray3.30R2-136[»]
4UKWX-ray3.10M2-136[»]
4UKYX-ray3.10R2-136[»]
4UL1X-ray3.60M2-136[»]
4UL3X-ray3.60R2-136[»]
4UL6X-ray3.00M2-136[»]
4UL8X-ray3.00R2-136[»]
4ULBX-ray3.00M2-136[»]
4ULDX-ray3.00R2-136[»]
4ULGX-ray3.20M2-136[»]
4ULIX-ray3.20R2-136[»]
4ULMX-ray3.10M2-136[»]
4ULOX-ray3.10R2-136[»]
4ULRX-ray3.20M2-136[»]
4ULTX-ray3.20Q2-136[»]
4V7Felectron microscopy8.70Y1-136[»]
4V8Telectron microscopy8.10Z1-136[»]
ProteinModelPortaliP0C2H6.
SMRiP0C2H6. Positions 2-136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L27e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010721.
HOGENOMiHOG000210138.
InParanoidiP0C2H6.
KOiK02901.
OMAiKIYKPGK.
OrthoDBiEOG7GXPR2.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR014722. Rib_L2_dom2.
IPR001141. Ribosomal_L27e.
IPR018262. Ribosomal_L27e_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR10497. PTHR10497. 1 hit.
PfamiPF01777. Ribosomal_L27e. 1 hit.
[Graphical view]
ProDomiPD009396. Ribosomal_L27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiPS01107. RIBOSOMAL_L27E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2H6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP
60 70 80 90 100
LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDVE AFKSVVSTET
110 120 130
FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF
Length:136
Mass (Da):15,531
Last modified:March 6, 2007 - v1
Checksum:iF3924B039AA2D85D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10400 Genomic DNA. Translation: AAB68944.1.
BK006934 Genomic DNA. Translation: DAA06699.1.
PIRiS46790.
RefSeqiNP_011874.1. NM_001179140.1.

Genome annotation databases

EnsemblFungiiYHR010W; YHR010W; YHR010W.
GeneIDi856401.
KEGGisce:YHR010W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10400 Genomic DNA. Translation: AAB68944.1.
BK006934 Genomic DNA. Translation: DAA06699.1.
PIRiS46790.
RefSeqiNP_011874.1. NM_001179140.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW8electron microscopy6.10a1-136[»]
1VWUelectron microscopy6.10a1-136[»]
1VXVelectron microscopy6.20a1-136[»]
1VXYelectron microscopy6.30a1-136[»]
3IZSelectron microscopy-a1-136[»]
3J65electron microscopy8.70Y1-136[»]
3U5EX-ray3.00Z1-136[»]
3U5IX-ray3.00Z1-136[»]
4B6Aelectron microscopy8.10Z1-136[»]
4BYNelectron microscopy4.30Z2-136[»]
4BYUelectron microscopy6.60Z2-136[»]
4CUWelectron microscopy3.70Z1-136[»]
4UJHX-ray3.20M2-136[»]
4UJJX-ray3.20R2-136[»]
4UJMX-ray3.00M2-136[»]
4UJOX-ray3.00R2-136[»]
4UJRX-ray2.80M2-136[»]
4UJTX-ray2.80R2-136[»]
4UJWX-ray3.10M2-136[»]
4UJYX-ray3.10Q2-136[»]
4UK1X-ray3.00M2-136[»]
4UK3X-ray3.00R2-136[»]
4UK6X-ray3.20M2-136[»]
4UK8X-ray3.20R2-136[»]
4UKBX-ray2.90M2-136[»]
4UKEX-ray2.90R2-136[»]
4UKHX-ray3.45M2-136[»]
4UKJX-ray3.45Q2-136[»]
4UKMX-ray3.20M2-136[»]
4UKOX-ray3.20Q2-136[»]
4UKRX-ray3.30M2-136[»]
4UKTX-ray3.30R2-136[»]
4UKWX-ray3.10M2-136[»]
4UKYX-ray3.10R2-136[»]
4UL1X-ray3.60M2-136[»]
4UL3X-ray3.60R2-136[»]
4UL6X-ray3.00M2-136[»]
4UL8X-ray3.00R2-136[»]
4ULBX-ray3.00M2-136[»]
4ULDX-ray3.00R2-136[»]
4ULGX-ray3.20M2-136[»]
4ULIX-ray3.20R2-136[»]
4ULMX-ray3.10M2-136[»]
4ULOX-ray3.10R2-136[»]
4ULRX-ray3.20M2-136[»]
4ULTX-ray3.20Q2-136[»]
4V7Felectron microscopy8.70Y1-136[»]
4V8Telectron microscopy8.10Z1-136[»]
ProteinModelPortaliP0C2H6.
SMRiP0C2H6. Positions 2-136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36437. 93 interactions.
IntActiP0C2H6. 4 interactions.
MINTiMINT-8285600.
STRINGi4932.YHR010W.

Proteomic databases

MaxQBiP0C2H6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR010W; YHR010W; YHR010W.
GeneIDi856401.
KEGGisce:YHR010W.

Organism-specific databases

SGDiS000001052. RPL27A.

Phylogenomic databases

GeneTreeiENSGT00390000010721.
HOGENOMiHOG000210138.
InParanoidiP0C2H6.
KOiK02901.
OMAiKIYKPGK.
OrthoDBiEOG7GXPR2.

Enzyme and pathway databases

BioCyciYEAST:G3O-31073-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

NextBioi981934.

Gene expression databases

GenevestigatoriP0C2H6.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR014722. Rib_L2_dom2.
IPR001141. Ribosomal_L27e.
IPR018262. Ribosomal_L27e_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR10497. PTHR10497. 1 hit.
PfamiPF01777. Ribosomal_L27e. 1 hit.
[Graphical view]
ProDomiPD009396. Ribosomal_L27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50104. SSF50104. 1 hit.
PROSITEiPS01107. RIBOSOMAL_L27E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  4. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL27A_YEAST
AccessioniPrimary (citable) accession number: P0C2H6
Secondary accession number(s): D3DKV5, P38706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: February 4, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7380 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L27 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.