ID   KBX3_HETLA              Reviewed;          95 AA.
AC   P0C2F4;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   24-JAN-2024, entry version 35.
DE   RecName: Full=Heteroscorpine-1 {ECO:0000303|PubMed:17056081};
DE            Short=HS-1 {ECO:0000303|PubMed:17056081};
DE   Flags: Precursor;
OS   Heterometrus laoticus (Thai giant scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Heterometrinae;
OC   Heterometrus.
OX   NCBI_TaxID=217256;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20; 58-71 AND 80-86,
RP   FUNCTION, MASS SPECTROMETRY, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=17056081; DOI=10.1016/j.toxicon.2006.09.003;
RA   Uawonggul N., Thammasirirak S., Chaveerach A., Arkaravichien T.,
RA   Bunyatratchata W., Ruangjirachuporn W., Jearranaiprepame P., Nakamura T.,
RA   Matsuda M., Kobayashi M., Hattori S., Daduang S.;
RT   "Purification and characterization of Heteroscorpine-1 (HS-1) toxin from
RT   Heterometrus laoticus scorpion venom.";
RL   Toxicon 49:19-29(2007).
CC   -!- FUNCTION: Has antibacterial activity against B.subtilis, K.pneumoniae
CC       and P.aeruginosa. {ECO:0000269|PubMed:17056081}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17056081}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:17056081}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:17056081}.
CC   -!- MASS SPECTROMETRY: Mass=8293; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17056081};
CC   -!- TOXIC DOSE: PD(50) is 80 ul to crickets (Gryllus sp).
CC       {ECO:0000269|PubMed:17056081}.
CC   -!- SIMILARITY: Belongs to the long chain scorpion toxin family. Class 3
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C2F4; -.
DR   SMR; P0C2F4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR029237; Long_scorpion_toxin.
DR   Pfam; PF14866; Toxin_38; 1.
DR   PROSITE; PS51862; BSPN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:17056081"
FT   CHAIN           20..95
FT                   /note="Heteroscorpine-1"
FT                   /evidence="ECO:0000305|PubMed:17056081"
FT                   /id="PRO_0000274680"
FT   DOMAIN          55..95
FT                   /note="BetaSPN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
FT   DISULFID        58..82
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
FT   DISULFID        68..87
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
FT   DISULFID        72..89
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
SQ   SEQUENCE   95 AA;  10246 MW;  21CCE2CD82EFFFA6 CRC64;
     MNSKLTALIF LGLVAIASCG WINEEKIQKK IDEKIGNNIL GGMAKAVVHK LAKGEFQCVA
     NIDTMGNCET HCQKTSGEKG FCHGTKCKCG KPLSY
//