P0C2F4 (KBX3_HETLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Heteroscorpine-1 Short name=HS-1
Organism	Heterometrus laoticus (Thai giant scorpion)
Taxonomic identifier	217256 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Scorpionoidea › Scorpionidae › Scorpioninae › Heterometrus

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has antibacterial activity against B.subtilis, K.pneumoniae and P.aeruginosa.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains 3 disulfide bonds.
Toxic dose	PD50 is 80 ul to crickets (Gryllus sp). Ref.1
Sequence similarities	Belongs to the long chain scorpion toxin family. Class 3 subfamily.
Mass spectrometry	Molecular mass is 8293 Da from positions 20 - 95. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 95	76	Heteroscorpine-1		PRO_0000274680
Amino acid modifications
	Disulfide bond	58 ↔ 82		By similarity
	Disulfide bond	68 ↔ 87		By similarity
	Disulfide bond	72 ↔ 89		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C2F4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 21CCE2CD82EFFFA6	FASTA	95	10,246
	10 20 30 40 50 60 MNSKLTALIF LGLVAIASCG WINEEKIQKK IDEKIGNNIL GGMAKAVVHK LAKGEFQCVA 70 80 90 NIDTMGNCET HCQKTSGEKG FCHGTKCKCG KPLSY

References

[1]

"Purification and characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus scorpion venom." Uawonggul N., Thammasirirak S., Chaveerach A., Arkaravichien T., Bunyatratchata W., Ruangjirachuporn W., Jearranaiprepame P., Nakamura T., Matsuda M., Kobayashi M., Hattori S., Daduang S. Toxicon 49:19-29(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20; 58-71 AND 80-86, MASS SPECTROMETRY, PARALYTIC DOSE. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

KBX3_HETLA

Accession

Primary (citable) accession number: P0C2F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	October 3, 2012
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)