P0C2F3 (KBX1_TITST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin Tst-beta-KTx Short name=TstbetaKTx
Organism	Tityus stigmurus (Brazilian scorpion)
Taxonomic identifier	50344 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Tityus

Protein attributes

Sequence length	60 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Blocks potassium channels Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.3/KCNA3. Ref.3
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Miscellaneous	This toxin does not inhibit Kv1.4/KCNA4, Kv1.5/KCNA5, Kv1.6/KCNA6, Shaker IR and Kv11.1/KCNH2 potassium channels. Does not show cytolytic activity (Ref.3).
Sequence similarities	Belongs to the long chain scorpion toxin family. Class 1 subfamily.
Mass spectrometry	Molecular mass is 6716.8 Da from positions 1 - 60. Determined by ESI. Ref.1 Ref.2

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 60	60	Potassium channel toxin Tst-beta-KTx		PRO_0000274675
Amino acid modifications
	Disulfide bond	29 ↔ 50		By similarity
	Disulfide bond	36 ↔ 55		By similarity
	Disulfide bond	40 ↔ 57		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C2F3 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: BA0F39860AB5CD21	FASTA	60	6,723
	10 20 30 40 50 60 KLVALIPNDQ LRSILKAVVH KVAKTQFGCP AYEGYCNDHC NDIERKDGEC HGFKCKCAKD

References

[1]	"Proteomic analysis of the venom from the scorpion Tityus stigmurus: biochemical and physiological comparison with other Tityus species." Batista C.V.F., Roman-Gonzalez S.A., Salas-Castillo S.P., Zamudio F.Z., Gomez-Lagunas F., Possani L.D. Comp. Biochem. Physiol. 146C:147-157(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.
[2]	"Wide phylogenetic distribution of scorpine and long-chain beta-KTx-like peptides in scorpion venoms: identification of 'orphan' components." Diego-Garcia E., Schwartz E.F., D'Suze G., Gonzalez S.A., Batista C.V., Garcia B.I., Rodriguez de la Vega R.C., Possani L.D. Peptides 28:31-37(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.
[3]	"Cytolytic and K+ channel blocking activities of beta-KTx and scorpine-like peptides purified from scorpion venoms." Diego-Garcia E., Abdel-Mottaleb Y., Schwartz E.F., Rodriguez de la Vega R.C., Tytgat J., Possani L.D. Cell. Mol. Life Sci. 65:187-200(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

KBX1_TITST

Accession

Primary (citable) accession number: P0C2F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	March 6, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program