Reviewed, UniProtKB/Swiss-Prot P0C2D7 (OXLA_VIPBB)

Names and origin

Protein names	Recommended name:     L-amino-acid oxidase       Short name=LAAO       Short name=LAO     EC=1.4.3.2
Organism	Vipera berus berus (Common viper)
Taxonomic identifier	31156 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Vipera

Protein attributes

Sequence length	88 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (the most specific substrate is L-Phe, followed by L-Met, L-Leu, L-Phe, L-Ile, L-Arg and L-His). Inhibits both ADP-induced platelet aggregation dose-dependently. Has an ability to induce apoptosis in cultured HeLa and K562 cells. May have an ability to induce hemorrhage. May have an antibacterial activity. Ref.1
Catalytic activity	An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Homodimer. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.

Ontologies

Keywords
Biological process	Apoptosis Blood coagulation
Cellular component	Secreted
Ligand	FAD Flavoprotein
Molecular function	Antibiotic Antimicrobial Oxidoreductase Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›88	›88	L-amino-acid oxidase		PRO_0000273573
Regions
	Nucleotide binding	83 – 86	4	FAD By similarity
Sites
	Binding site	74	1	FAD By similarity
Experimental info
	Non-adjacent residues	37 – 38	2
	Non-adjacent residues	44 – 45	2
	Non-adjacent residues	52 – 53	2
	Non-adjacent residues	61 – 62	2
	Non-adjacent residues	68 – 69	2
	Non-terminal residue	88	1

Sequences

	Sequence		Length	Mass (Da)
	P0C2D7-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: D2A63168391A7965	FASTA	88	10,295
	10 20 30 40 50 60 ADDKNPLEEC FREDDYEEFL EIAKNGLKKT SNPKHIVYPV KPSEQLYEES LRDQLPTSMH 70 80 RYPSMIQKIF FAGEYTANAH GWIDSTIK

References

[1]

"Isolation and characterization of an apoptotic and platelet aggregation inhibiting L-amino acid oxidase from Vipera berus berus (common viper) venom." Samel M., Vija H., Ronnholm G., Siigur J., Kalkkinen N., Siigur E. Biochim. Biophys. Acta 1764:707-714(2006) [PubMed: 16574513] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P0C2D7.
Enzyme and pathway databases
BRENDA	1.4.3.2. 292016.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

OXLA_VIPBB

Accession

Primary (citable) accession number: P0C2D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)