Reviewed, UniProtKB/Swiss-Prot P0C2D4 (OXLA_NAJKA)

Names and origin

Protein names	Recommended name:     L-amino-acid oxidase       Short name=LAAO       Short name=LAO       Short name=K-LAO     EC=1.4.3.2
Organism	Naja kaouthia (Monocled cobra) (Naja siamensis)
Taxonomic identifier	8649 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Elapinae › Naja

Protein attributes

Sequence length	38 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Is very active against L-Phe and L-Tyr, moderately active against L-Trp, L-Met, L-Leu, and L-Arg. Inhibits both agonist- and shear stress-induced platelet aggregation (SIPA) dose-dependently through the formation of hydrogen peroxide (H2O2). May induce apoptosis and hemorrhage. May have an antibacterial activity. Ref.2
Catalytic activity	An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Homodimer. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.
biophysicochemical properties	pH dependence: Optimum pH is 8.5, unlike many other venom L-amino acid oxidase, is also stable in alkaline medium.

Ontologies

Keywords
Biological process	Apoptosis Blood coagulation
Cellular component	Secreted
Ligand	FAD Flavoprotein
Molecular function	Antibiotic Antimicrobial Oxidoreductase Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›38	›38	L-amino-acid oxidase		PRO_0000273569
Experimental info
	Non-terminal residue	38	1

Sequences

	Sequence		Length	Mass (Da)
	P0C2D4-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: D988B9FC32DDDE08	FASTA	38	4,514
	10 20 30 DDRRSPLEEC FQQNDYEEFL EIAKNGLKKT XNPKHVXV

References

[1]	"Inhibition of human platelet aggregation by L-amino acid oxidase purified from Naja naja kaouthia venom." Sakurai Y., Takatsuka H., Yoshioka A., Matsui T., Suzuki M., Titani K., Fujimura Y. Toxicon 39:1827-1833(2001) [PubMed: 11600144] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Purification and properties of the L-amino acid oxidase from monocellate cobra (Naja naja kaouthia) venom." Tan N.-H., Swaminathan S. Int. J. Biochem. 24:967-973(1992) [PubMed: 1612186] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P0C2D4.
Enzyme and pathway databases
BRENDA	1.4.3.2. 274888.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

OXLA_NAJKA

Accession

Primary (citable) accession number: P0C2D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)