P0C2B2 (DSBA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiol:disulfide interchange protein DsbA

Gene names

Name:	dsbA
Ordered Locus Names:	PA5489

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Chain

23 – 211

189

Thiol:disulfide interchange protein DsbA

PRO_0000034262

Regions

Domain

23 – 203

181

Thioredoxin

Amino acid modifications

Disulfide bond

56 ↔ 59

Redox-active Potential

Experimental info

Sequence conflict

209

1

Missing Ref.1

Secondary structure

1

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211

Helix Strand Turn

Details...

Helix

22 – 24

3

Beta strand

31 – 33

3

Beta strand

46 – 52

7

Helix

57 – 71

15

Beta strand

77 – 83

7

Turn

90 – 92

3

Helix

96 – 102

7

Helix

107 – 118

12

Helix

127 – 134

8

Helix

141 – 148

8

Turn

151 – 153

3

Helix

157 – 167

11

Beta strand

174 – 177

4

Beta strand

181 – 184

4

Helix

192 – 210

19

Sequences

	Sequence		Length	Mass (Da)
	P0C2B2 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: A994F93CC8E8C9F6	FASTA	211	23,375
	10 20 30 40 50 60 MRNLILTAML AMASLFGMAA QADDYTAGKE YVELSSPVPV SQPGKIEVVE LFWYGCPHCY 70 80 90 100 110 120 AFEPTIVPWS EKLPADVHFV RLPALFGGIW NVHGQMFLTL ESMGVEHDVH NAVFEAIHKE 130 140 150 160 170 180 HKKLATPEEM ADFLAGKGVD KEKFLSTYNS FAIKGQMEKA KKLAMAYQVT GVPTMVVNGK 190 200 210 YRFDIGSAGG PEETLKLADY LIEKERAAAK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a dsbA-homologous gene from Pseudomonas aeruginosa." Leipelt M., Schneidinger B., Jaeger K.-E. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U84726 Genomic DNA. Translation: AAB41795.1.
AE004091 Genomic DNA. Translation: AAG08874.1.

PIR

H82960.

RefSeq

NP_254176.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3H93	X-ray	1.50	A	22-211	[»]

ProteinModelPortal

P0C2B2.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

877731.

GenomeReviews

Gene locus PA5489 in contig AE004091_GR.

KEGG

pae:PA5489.

PATRIC

19845865. VBIPseAer58763_5754.

Organism-specific databases

PseudoCAP

PA5489.

Phylogenomic databases

HOGENOM

HBG680642.

OMA

EVPKIKA.

ProtClustDB

CLSK869391.

Enzyme and pathway databases

BioCyc

PAER208964:PA5489-MONOMER.

Family and domain databases

InterPro

IPR001853. DSBA-like_thioredoxin_dom.
IPR023205. Thiol:disulphide_interchange.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.

KO

K03673.

Pfam

PF01323. DSBA. 1 hit.
[Graphical view]

PIRSF

PIRSF001488. Tdi_protein. 1 hit.

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DSBA_PSEAE

Accession

Primary (citable) accession number: P0C2B2
Secondary accession number(s): P95460

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program