Reviewed, UniProtKB/Swiss-Prot P0C2B2 (DSBA_PSEAE)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbA

Gene names

Name:	dsbA
Ordered Locus Names:	PA5489

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 211	189	Thiol:disulfide interchange protein dsbA		PRO_0000034262
Regions
	Domain	23 – 203	181	Thioredoxin
Amino acid modifications
	Disulfide bond	56 ↔ 59		Redox-active Potential
Experimental info
	Sequence conflict	209	1	Missing Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C2B2-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: A994F93CC8E8C9F6	FASTA	211	23,375
	10 20 30 40 50 60 MRNLILTAML AMASLFGMAA QADDYTAGKE YVELSSPVPV SQPGKIEVVE LFWYGCPHCY 70 80 90 100 110 120 AFEPTIVPWS EKLPADVHFV RLPALFGGIW NVHGQMFLTL ESMGVEHDVH NAVFEAIHKE 130 140 150 160 170 180 HKKLATPEEM ADFLAGKGVD KEKFLSTYNS FAIKGQMEKA KKLAMAYQVT GVPTMVVNGK 190 200 210 YRFDIGSAGG PEETLKLADY LIEKERAAAK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a dsbA-homologous gene from Pseudomonas aeruginosa." Leipelt M., Schneidinger B., Jaeger K.-E. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U84726 Genomic DNA. Translation: AAB41795.1. AE004091 Genomic DNA. Translation: AAG08874.1.
PIR	H82960.
RefSeq	NP_254176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	877731.
GenomeReviews	Gene locus PA5489 in contig AE004091_GR.
KEGG	pae:PA5489.
Organism-specific databases
PseudoCAP	PA5489.
CMR	Search...
Phylogenomic databases
HOGENOM	P0C2B2.
OMA	P0C2B2. GKWALSQ.
Enzyme and pathway databases
BioCyc	PAER208964:PA5489-MON.
Family and domain databases
InterPro	IPR001853. OxRdtase_DSBA. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF01323. DSBA. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBA_PSEAE

Accession

Primary (citable) accession number: P0C2B2
Secondary accession number(s): P95460

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)