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Protein

Kappa-theraphotoxin-Cg1a 1

Gene
N/A
Organism
Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys jingzhao)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This toxin acts as a voltage-dependent gating-modifier (PubMed:25240294). It inhibits the sodium conductance (IC50=124 nM) and slows the fast inactivation (EC(50)=1180 nM) of Nav1.5/SCN5A (PubMed:17176080, PubMed:25240294). It significantly shifts the activation to more depolarized voltages and decreases the deactivation of Nav1.5 currents upon extreme depolarization, but only slightly affects voltage-dependence of steady-state inactivation (PubMed:17176080, PubMed:25240294). In addition, this toxin causes an approximately five-fold decrease in the rate of recovery from inactivation and an approximately 1.9-fold reduction in the closed-state inactivation rate (PubMed:25240294). This toxin integrates the functions of site 3 toxins (alpha-scorpion toxins) with site 4 toxins (beta-scorpion and spider toxins) by targeting multiple sites on Nav1.5 (PubMed:25240294). Also shows inhibition of voltage-gated potassium channels (5 µM completely inhibits Kv2.1/KCNB1, whereas 5 µM moderately inhibits Kv4.2/KCND2 Kv4.1/KCND1 channels) (PubMed:17176080).2 Publications

Miscellaneous

This toxin does not show effect on Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3, Kv1.4/KCNA4, Kv3.1/KCNC1 (all expressed in oocytes), voltage-gated sodium channels (Nav1/SCN) (from DRG neurons), and in a range of voltage-gated calcium channels (expressed in rat DRG neurons) (PubMed:17176080). Does not show significant toxic symptoms when injected into mice and into cockroaches (PubMed:17176080).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei80Involved in active faceBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBi8.B.5.3.5. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Cg1a 11 Publication
Short name:
Kappa-TRTX-Cg1a1 Publication
Alternative name(s):
Jingzhaotoxin-11
Short name:
JZTX-11
Jingzhaotoxin-XI1 Publication
Short name:
JZTX-XI1 Publication
Peptide F4-13.641 Publication
OrganismiChilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys jingzhao)
Taxonomic identifieri278060 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeChilobrachys

Organism-specific databases

ArachnoServeriAS000043. kappa-theraphotoxin-Cg1a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000028743122 – 502 PublicationsAdd BLAST29
PeptideiPRO_000026245651 – 84Kappa-theraphotoxin-Cg1a 13 PublicationsAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 661 PublicationImported
Disulfide bondi59 ↔ 711 PublicationImported
Disulfide bondi65 ↔ 781 PublicationImported
Modified residuei84Phenylalanine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.2 Publications

Structurei

Secondary structure

186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi62 – 64Combined sources3
Beta strandi73 – 78Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A2VNMR-A51-84[»]
ProteinModelPortaliP0C247.
SMRiP0C247.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C247.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 56Involved in active faceBy similarity2

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.1 Publication

Sequence similaritiesi

Belongs to the huwentoxin-1 family. Jztx-11 subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSVLITLA VLGVMFVWAS AAELEERGSD QRDSPAWLKS MERIFQSGER
60 70 80
ECRKMFGGCS VDSDCCAHLG CKPTLKYCAW DGTFGK
Length:86
Mass (Da):9,527
Last modified:May 15, 2007 - v2
Checksum:iFD137D8FD06B9AD9
GO

Mass spectrometryi

P0C247: Molecular mass is 3726.38 Da from positions 51 - 84. Determined by MALDI. 1 Publication

Similar proteinsi

Entry informationi

Entry nameiJZ11A_CHIGU
AccessioniPrimary (citable) accession number: P0C247
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 15, 2007
Last modified: October 25, 2017
This is version 50 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families