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Protein

Protein Rex

Gene
N/A
Organism
Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rex escorts unspliced gag-pro-pol and singly spliced env mRNAs out of the nucleus of infected cells. These mRNAs carry a recognition sequence called Rex responsive element (RxRE or XRE) located at the 3' region of the long terminal repeat (LTR). This function is essential since most HTLV proteins are translated from unspliced or partially spliced pre-mRNAs that cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rex itself is translated from a fully spliced mRNA that probably readily exits the nucleus. Rex's nuclear localization signal (NLS) binds directly to KPNB1/importin beta-1 without previous binding to KPNA1/importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rex to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rex from KPNB1 and allows Rex's binding to the RRE in viral pre-mRNAs. Rex multimerizes on the RRE via cooperative assembly. This multimerization is critical for its full biological activity, since it may shield the viral RNA from being spliced or down-regulated, and probably exposes Rex's nuclear export signal (NES) to the surface. Rex can then form a complex with XPO1/CRM1, RANBP3 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rex/RRE/XPO1/RANBP3/RAN complex, so that Rex can return to the nucleus for a subsequent round of export (Probable).Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, mRNA transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Rex
Alternative name(s):
Rev homolog
Rex-1
p27Rex
OrganismiHuman T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1)
Taxonomic identifieri11926 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeDeltaretrovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007683 Componenti: Genome

Subcellular locationi

Isoform Rex :

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85Q → A: No effect on mRNA export. 1 Publication1
Mutagenesisi85Q → P: Complete loss of mRNA export. 1 Publication1
Mutagenesisi86L → A: Complete loss of mRNA export; when associated with A-90. 1 Publication1
Mutagenesisi90L → A: Complete loss of mRNA export; when associated with A-86. 1 Publication1
Mutagenesisi91S → P: Complete loss of mRNA export. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002597821 – 189Protein RexAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70Phosphoserine; by host1 Publication1
Modified residuei174Phosphothreonine; by host1 Publication1
Modified residuei177Phosphoserine; by host1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP0C205.

Expressioni

Inductioni

Down-regulated by P30II.

Interactioni

Subunit structurei

Homomultimer. Multimeric assembly is essential for activity and involves XPO1. Binds to human XPO1 and KPNB1. Interacts (via N-terminal nuclear localization signal) with human NPM1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DICER1Q9UPY37EBI-8332963,EBI-395506From Homo sapiens.

Protein-protein interaction databases

IntActiP0C205. 1 interactor.
MINTiMINT-7996809.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 70HomomultimerizationBy similarityAdd BLAST15
Regioni123 – 131HomomultimerizationBy similarity9

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2 – 18Nuclear localization signal, and RNA-binding (RxRE)Add BLAST17
Motifi82 – 93Nuclear export signalAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi95 – 188Pro-richAdd BLAST94

Domaini

The RNA-binding motif of Rex binds to the RxRE, a complex secondary structure consisting of four stem loops and a long stretch of stem structure, present in incompletely spliced viral pre-mRNAs. This region also contains the NLS which mediates nuclear localization. These overlapping functions prevent Rex bound to RxRE from undesirable return to the nucleus. When Rex binds the RxRE, the NLS becomes masked while the NES remains accessible. The leucine-rich NES mediates binding to human XPO1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the deltaretrovirus Rex protein family.Curated

Phylogenomic databases

OrthoDBiVOG0900016H.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Rex (identifier: P0C205-1) [UniParc]FASTAAdd to basket
Also known as: p27Rex

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKTRRRPRR SQRKRPPTPW PTSQGLDRVF FSDTQSTCLE TVYKATGAPS
60 70 80 90 100
LGDYVRPAYI VTPYWPPVQS IRSPGTPSMD ALSAQLYSSL SLDSPPSPPR
110 120 130 140 150
EPLRPSRSLP RQSLIQPPTF HPPSSRPCAN TPPSEMDTWN PPLGSTSQPC
160 170 180
LFQTPDSGPK TCTPSGEAPL SACTSTSFPP PSPGPSCPT
Length:189
Mass (Da):20,499
Last modified:November 14, 2006 - v1
Checksum:i3DA522C115C6E233
GO
Isoform p21Rex (identifier: P0C205-2) [UniParc]FASTAAdd to basket
Also known as: p21

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:111
Mass (Da):11,640
Checksum:iF57B724A8D618093
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0215391 – 78Missing in isoform p21Rex. CuratedAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02029 Genomic DNA. No translation available.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02029 Genomic DNA. No translation available.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0C205. 1 interactor.
MINTiMINT-7996809.

PTM databases

iPTMnetiP0C205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG0900016H.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiREX_HTL1A
AccessioniPrimary (citable) accession number: P0C205
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: November 14, 2006
Last modified: March 15, 2017
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

HTLV-1 lineages are divided in four clades, A (Cosmopolitan), B (Central African group), C (Melanesian group) and D (New Central African group).

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.