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Protein

Fasciculin-2

Gene
N/A
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1:1 stoichiometry the mammalian and electric fish AChE at picomolar concentrations. It is highly specific for the peripheral site of AChE and blocks the entry of acetylcholine into the active site of the enzyme (through the Met-33 residue), thereby preventing its breakdown. It has been called fasciculin since after injection into mice it causes severe, generalized and long-lasting (5-7 hours) fasciculations.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Blocks the entrance of the active site gorge of hAChE1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionToxin

Names & Taxonomyi

Protein namesi
Recommended name:
Fasciculin-2
Short name:
Fas-2
Short name:
Fas2
Alternative name(s):
Acetylcholinesterase toxin F-VII
Fasciculin-II
Short name:
FAS-II
Toxin TA1
OrganismiDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Taxonomic identifieri8618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is >20 mg/kg by intravenous injection.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8 – 9TT → AA: 18-fold increase in inhibition potency. 1 Publication2
Mutagenesisi8T → V: 2.5-fold increase in affinity for TcAChE. 2.1-fold decrease in affinity for TcAChE; when associated with N-9. 1.9-fold increase in affinity for TcAChE; when associated with N-9, K-11 and R-29 [FasDesK32]. 3.9-fold decrease in affinity for TcAChE; when associated with N-9, K-11, R-29 and R-32 [FasDes]. 1 Publication1
Mutagenesisi9T → N: 9.0-fold decrease in affinity for TcAChE. 2.1-fold decrease in affinity for TcAChE; when associated with V-8. 1.9-fold increase in affinity for TcAChE; when associated with V-8, K-11 and R-29 [FasDesK32]. 3.9-fold decrease in affinity for TcAChE; when associated with V-8, K-11, R-29 and R-32 [FasDes]. 1 Publication1
Mutagenesisi11R → K: 1.7-fold decrease in affinity for TcAChE. 1.9-fold increase in affinity for TcAChE; when associated with V-8, N-9 and R-29 [FasDesK32]. 3.9-fold decrease in affinity for TcAChE; when associated with V-8, N-9, R-29 and R-32 [FasDes]. 1 Publication1
Mutagenesisi11R → Q: 6-fold increase in inhibition potency. 1 Publication1
Mutagenesisi24R → T: 13-fold decrease in inhibition potency. 1 Publication1
Mutagenesisi25K → L: No significant difference in inhibition potency. 1 Publication1
Mutagenesisi27R → W: 49-fold decrease in inhibition potency. 1 Publication1
Mutagenesisi28R → D: No significant difference in inhibition potency. 1 Publication1
Mutagenesisi29H → D: 73-fold increase in inhibition potency. 1 Publication1
Mutagenesisi29H → R: 6.0-fold increase in affinity for TcAChE. 1.9-fold increase in affinity for TcAChE; when associated with V-8, N-9 and K-11 [FasDesK32]. 3.9-fold decrease in affinity for TcAChE; when associated with V-8, N-9, K-11 and R-32 [FasDes]. 1 Publication1
Mutagenesisi30Missing : 192-fold decrease in inhibition potency. 1 Publication1
Mutagenesisi31P → R: 625-fold decrease in inhibition potency. 1 Publication1
Mutagenesisi32K → G: 3-fold decrease in inhibition potency. 1 Publication1
Mutagenesisi32K → R: 7.4-fold decrease in affinity for TcAChE. 3.9-fold decrease in affinity for TcAChE; when associated with V-8, N-9, K-11 and R-29 [FasDes]. 1 Publication1
Mutagenesisi33M → A: 8-fold decrease in inhibition potency. 1 Publication1
Mutagenesisi34 – 35VL → AA: No significant difference in inhibition potency. 1 Publication2
Mutagenesisi45D → K: No significant difference in inhibition potency. 1 Publication1
Mutagenesisi51K → S: No significant difference in inhibition potency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002530311 – 61Fasciculin-2Add BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 228 Publications
Disulfide bondi17 ↔ 398 Publications
Disulfide bondi41 ↔ 528 Publications
Disulfide bondi53 ↔ 598 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Beta strandi8 – 10Combined sources3
Beta strandi13 – 16Combined sources4
Beta strandi22 – 31Combined sources10
Beta strandi34 – 40Combined sources7
Beta strandi46 – 53Combined sources8
Turni57 – 60Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B41X-ray2.76B1-61[»]
1F8UX-ray2.90B1-61[»]
1FSCX-ray2.00A1-61[»]
1FSSX-ray3.00B1-61[»]
1KU6X-ray2.50B1-61[»]
1MAHX-ray3.20F1-61[»]
2X8BX-ray2.95B1-61[»]
4BDTX-ray3.10B1-61[»]
4EY8X-ray2.60B1-61[»]
ProteinModelPortaliP0C1Z0.
SMRiP0C1Z0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C1Z0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiView protein in InterPro
IPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
PROSITEiView protein in PROSITE
PS00272. SNAKE_TOXIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C1Z0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TMCYSHTTTS RAILTNCGEN SCYRKSRRHP PKMVLGRGCG CPPGDDNLEV
60
KCCTSPDKCN Y
Length:61
Mass (Da):6,758
Last modified:October 17, 2006 - v1
Checksum:i50A38EF3633C383F
GO

Sequence databases

PIRiA01674. T4EP1A.

Similar proteinsi

Entry informationi

Entry namei3SE2_DENAN
AccessioniPrimary (citable) accession number: P0C1Z0
Secondary accession number(s): P01403
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: September 27, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families