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P0C1Y0 (BGAL_LACDE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismLactobacillus delbrueckii subsp. bulgaricus
Taxonomic identifier1585 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length1007 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subunit structure

Monomer.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 10071006Beta-galactosidase
PRO_0000057664

Sites

Active site4651Proton donor By similarity
Active site5321Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C1Y0 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: D8E306D2A24071AE

FASTA1,007114,047
        10         20         30         40         50         60 
MSNKLVKEKR VDQADLAWLT DPEVYEVNTI PPHSDHESFQ SQEELEEGKS SLVQSLDGDW 

        70         80         90        100        110        120 
LIDYAENGQG PVNFYAEDFD DSNFKSVKVP GNLELQGFGQ PQYVNVQYPW DGSEEIFPPQ 

       130        140        150        160        170        180 
IPSKNPLASY VRYFDLDEAF WDKEVSLKFD GAATAIYVWL NGHFVGYGED SFTPSEFMVT 

       190        200        210        220        230        240 
KFLKKENNRL AVALYKYSSA SWLEDQDFWR MSGLFRSVTL QAKPRLHLED LKLTASLTDN 

       250        260        270        280        290        300 
YQKGKLEVEA NIAYRLPNAS FKLEVRDSEG DLVAEKLGPI RSEQLEFTLA DLPVAAWSAE 

       310        320        330        340        350        360 
KPNLYQVRLY LYQAGSLLEV SRQEVGFRNF ELKDGIMYLN GQRIVFKGAN RHEFDSKLGR 

       370        380        390        400        410        420 
AITEEDMIWD IKTMKRSNIN AVRCSHYPNQ SLFYRLCDKY GLYVIDEANL ESHGTWEKVG 

       430        440        450        460        470        480 
GHEDPSFNVP GDDQHWLGAS LSRVKNMMAR DKNHASILIW SLGNESYAGT VFAQMADYVR 

       490        500        510        520        530        540 
KADPTRVQHY EGVTHNRKFD DATQIESRMY APAKVIEEYL TNKPAKPFIS VEYAHAMGNS 

       550        560        570        580        590        600 
VGDLAAYTAL EKYPHYQGGF IWDWIDQGLE KDGHLLYGGD FDDRPTDYEF CGNGLVFADR 

       610        620        630        640        650        660 
TESPKLANVK ALYANLKLEV KDGQLFLKND NLFTNSSSYY FLTSLLVDGK LTYQSRPLTF 

       670        680        690        700        710        720 
GLEPGESGTF ALPWPEVADE KGEVVYRVTA HLKEDLPWAD EGFTVAEAEE VAQKLPEFKP 

       730        740        750        760        770        780 
EGRPDLVDSD YNLGLKGNNF QILFSKVKGW PVSLKYAGRE YLKRLPEFTF WRALTDNDRG 

       790        800        810        820        830        840 
AGYGYDLARW ENAGKYARLK DISCEVKEDS VLVKTAFTLP VALKGDLTVT YEVDGRGKIA 

       850        860        870        880        890        900 
VTADFPGAEE AGLLPAFGLN LALPKELTDY RYYGLGPNES YPDRLEGNYL GIYQGAVKKN 

       910        920        930        940        950        960 
FSPYRPQETG NRSKVRWYQL FDEKGGLEFT ANGADLNLSA LPYSAAQIEA ADHAFELTNN 

       970        980        990       1000 
YTWVRALSAQ MGVGGDDSWG QKVHPEFCLD AQKARQLRLV IQPLLLK 

« Hide

References

[1]"Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli."
Schmidt B.F., Adams R.M., Requadt C., Power S., Mainzer S.E.
J. Bacteriol. 171:625-635(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-30.
Strain: B131.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M23530 Genomic DNA. Translation: AAA25240.1.
PIRA30093.

3D structure databases

ProteinModelPortalP0C1Y0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP0C1Y0.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_LACDE
AccessionPrimary (citable) accession number: P0C1Y0
Secondary accession number(s): P20043
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries