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Protein

Kunitz-type conkunitzin-S1

Gene
N/A
Organism
Conus striatus (Striated cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Blocks specifically voltage-activated potassium channels (Kv) of the Shaker family (IC50=1.33 nM).1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Protease inhibitor, Serine protease inhibitor, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type conkunitzin-S1
Short name:
Conk-S1
OrganismiConus striatus (Striated cone)
Taxonomic identifieri6493 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000024980527 – 86Kunitz-type conkunitzin-S1Add BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 83PROSITE-ProRule annotation1 Publication
Disulfide bondi58 ↔ 79PROSITE-ProRule annotation1 Publication
Modified residuei86Threonine amide1 Publication1

Post-translational modificationi

Contains 2 disulfide bonds instead of 3, as for all Kunitz domain proteins. A double Cys-mutant carrying an additional Cys bridge does not show difference in activity with the natural peptide. However, there are some differences in the kinetics of binding of both peptides to the channel (PubMed:15833744).1 Publication

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 34Combined sources4
Beta strandi46 – 52Combined sources7
Turni53 – 56Combined sources4
Beta strandi57 – 63Combined sources7
Beta strandi65 – 67Combined sources3
Beta strandi73 – 75Combined sources3
Helixi76 – 83Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y62X-ray2.45A/B/C/D/E/F27-86[»]
2CA7NMR-A27-86[»]
ProteinModelPortaliP0C1X2.
SMRiP0C1X2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C1X2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 83BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Sequence similaritiesi

Belongs to the venom Kunitz-type family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd00109. KU. 1 hit.
Gene3Di4.10.410.10. 1 hit.
InterProiView protein in InterPro
IPR002223. Kunitz_BPTI.
PfamiView protein in Pfam
PF00014. Kunitz_BPTI. 1 hit.
PRINTSiPR00759. BASICPTASE.
SMARTiView protein in SMART
SM00131. KU. 1 hit.
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiView protein in PROSITE
PS50279. BPTI_KUNITZ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C1X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRRFAAVL ILTICMLAPG TGTLLPKDRP SLCDLPADSG SGTKAEKRIY
60 70 80
YNSARKQCLR FDYTGQGGNE NNFRRTYDCQ RTCLYT
Length:86
Mass (Da):9,661
Last modified:September 19, 2006 - v1
Checksum:i69BEDED1DFE63355
GO

Entry informationi

Entry nameiVKTS1_CONST
AccessioniPrimary (citable) accession number: P0C1X2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: August 30, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families