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Protein

Kunitz-type conkunitzin-S1

Gene
N/A
Organism
Conus striatus (Striated cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Blocks specifically voltage-activated potassium channels (Kv) of the Shaker family (IC50=1.33 nM).1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Protease inhibitor, Serine protease inhibitor, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type conkunitzin-S1
Short name:
Conk-S1
OrganismiConus striatus (Striated cone)
Taxonomic identifieri6493 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 8660Kunitz-type conkunitzin-S1PRO_0000249805Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 83PROSITE-ProRule annotation1 Publication
Disulfide bondi58 ↔ 79PROSITE-ProRule annotation1 Publication
Modified residuei86 – 861Threonine amide1 Publication

Post-translational modificationi

Contains 2 disulfide bonds instead of 3, as for all Kunitz domain proteins. A double Cys-mutant carrying an additional Cys bridge does not show difference in activity with the natural peptide. However, there are some differences in the kinetics of binding of both peptides to the channel (PubMed:15833744).1 Publication

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

1
86
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 344Combined sources
Beta strandi46 – 527Combined sources
Turni53 – 564Combined sources
Beta strandi57 – 637Combined sources
Beta strandi65 – 673Combined sources
Beta strandi73 – 753Combined sources
Helixi76 – 838Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y62X-ray2.45A/B/C/D/E/F27-86[»]
2CA7NMR-A27-86[»]
ProteinModelPortaliP0C1X2.
SMRiP0C1X2. Positions 27-86.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C1X2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 8351BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the venom Kunitz-type family.Curated
Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiIPR002223. Kunitz_BPTI.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiPS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C1X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRRFAAVL ILTICMLAPG TGTLLPKDRP SLCDLPADSG SGTKAEKRIY
60 70 80
YNSARKQCLR FDYTGQGGNE NNFRRTYDCQ RTCLYT
Length:86
Mass (Da):9,661
Last modified:September 19, 2006 - v1
Checksum:i69BEDED1DFE63355
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y62X-ray2.45A/B/C/D/E/F27-86[»]
2CA7NMR-A27-86[»]
ProteinModelPortaliP0C1X2.
SMRiP0C1X2. Positions 27-86.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP0C1X2.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiIPR002223. Kunitz_BPTI.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiPS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVKTS1_CONST
AccessioniPrimary (citable) accession number: P0C1X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: December 9, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.