Reviewed, UniProtKB/Swiss-Prot P0C1V6 (VHUU_METKA)

Names and origin

Protein names

Recommended name:
    F420-non-reducing hydrogenase vhu subunit U
    EC=1.12.99.-

Gene names

Name:	vhuU
Ordered Locus Names:	MK0177.1

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	42 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase vhu is composed of four subunits; vhuA, vhuD, vhuG and vhuU By similarity.
Miscellaneous	The large subunit of vhu is split into vhuA and vhuU. Each contributes two ligands to the [NiFeSe] center.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Coding sequence diversity	Selenocysteine
Ligand	Metal-binding Nickel Selenium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 27	27	F420-non-reducing hydrogenase vhu subunit U		PRO_0000249594
	Propeptide	28 – 42	15	Removed in mature form By similarity		PRO_0000249595
Sites
	Metal binding	21	1	Nickel Potential
	Metal binding	24	1	Nickel Potential
Amino acid modifications
	Non-standard residue	21	1	Selenocysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	P0C1V6-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 6D0614F1CD624BF1	FASTA	42	5,092
	10 20 30 40 MAEKRDVLNY LEMVIRSYDI ULSCAAHVLD RVKFRIERKD ED

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010317 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0C1V6.
Family and domain databases
InterPro	IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
PROSITE	PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHUU_METKA

Accession

Primary (citable) accession number: P0C1V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 14 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)