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Protein

DNA topoisomerase 4 subunit A

Gene

parC

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Transition state stabilizerUniRule annotation1
Active sitei119O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit AUniRule annotation
Gene namesi
Name:parCUniRule annotation
Synonyms:grlA
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4088.
DrugBankiDB00537. Ciprofloxacin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454141 – 800DNA topoisomerase 4 subunit AAdd BLAST800

Proteomic databases

PRIDEiP0C1U9.

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39Interaction with DNAUniRule annotation1
Sitei75Interaction with DNAUniRule annotation1
Sitei77Interaction with DNAUniRule annotation1
Sitei88Interaction with DNAUniRule annotation1
Sitei94Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi93062.SACOL1390.

Chemistry databases

BindingDBiP0C1U9.

Structurei

3D structure databases

ProteinModelPortaliP0C1U9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00937. ParC_type2. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR005741. TopoIV_A_Gpos.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 5 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01061. parC_Gpos. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C1U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEIIQDLSL EDVLGDRFGR YSKYIIQERA LPDVRDGLKP VQRRILYAMY
60 70 80 90 100
SSGNTHDKNF RKSAKTVGDV IGQYHPHGDF SVYKAMVRLS QDWKLRHVLI
110 120 130 140 150
EMHGNNGSID NDPPAAMRYT EAKLSLLAEE LLRDINKETV SFIPNYDDTT
160 170 180 190 200
LEPMVLPSRF PNLLVNGSTG ISAGYATDIP PHNLAEVIQA TLKYIDNPDI
210 220 230 240 250
TVNQLMKYIK GPDFPTGGII QGIDGIKKAY ESGKGRIIVR SKVEEETLRN
260 270 280 290 300
GRKQLIITEI PYEVNKSSLV KRIDELRADK KVDGIVEVRD ETDRTGLRIA
310 320 330 340 350
IELKKDVNSE SIKNYLYKNS DLQISYNFNM VAISDGRPKL MGIRQIIDSY
360 370 380 390 400
LNHQIEVVAN RTKFELDNAE KRMHIVEGLI KALSILDKVI ELIRSSKNKR
410 420 430 440 450
DAKENLIEVF EFTEEQAEAI VMLQLYRLTN TDIVALEGEH KELEALIKQL
460 470 480 490 500
RHILDNHDAL LNVIKEELNE IKKKFKSERL SLIEAEIEEI KIDKEVMVPS
510 520 530 540 550
EEVILSMTRH GYIKRTSIRS FNASGVEDIG LKDGDSLLKH QEVNTQDTVL
560 570 580 590 600
VFTNKGRYLF IPVHKLADIR WKELGQHVSQ IVPIEEDEVV INVFNEKDFN
610 620 630 640 650
TDAFYVFATQ NGMIKKSTVP LFKTTRFNKP LIATKVKEND DLISVMRFEK
660 670 680 690 700
DQLITVITNK GMSLTYNTSE LSDTGLRAAG VKSINLKAED FVVMTEGVSE
710 720 730 740 750
NDTILMATQR GSLKRISFKI LQVAKRAQRG ITLLKELKKN PHRIVAAHVV
760 770 780 790 800
TGEHSQYTLY SKSNEEHGLI NDIHKSEQYT NGSFIVDTDD FGEVIDMYIS
Length:800
Mass (Da):91,041
Last modified:September 5, 2006 - v1
Checksum:i01A59C49A81A4148
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti267S → G in AAA53116 (PubMed:7997176).Curated1
Sequence conflicti410F → Y in AAA53116 (PubMed:7997176).Curated1
Sequence conflicti567A → R in AAA53116 (PubMed:7997176).Curated1
Sequence conflicti594F → Y in AAA53116 (PubMed:7997176).Curated1
Sequence conflicti688A → V in AAA53116 (PubMed:7997176).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti80F → S in strain: FDA 574. 1
Natural varianti84K → E in strain: FDA 574. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25288 Genomic DNA. Translation: AAA53116.1.
D67074 Genomic DNA. Translation: BAA11085.1.
PIRiS54427.
RefSeqiWP_001289554.1. NZ_JYFP01000006.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25288 Genomic DNA. Translation: AAA53116.1.
D67074 Genomic DNA. Translation: BAA11085.1.
PIRiS54427.
RefSeqiWP_001289554.1. NZ_JYFP01000006.1.

3D structure databases

ProteinModelPortaliP0C1U9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL1390.

Chemistry databases

BindingDBiP0C1U9.
ChEMBLiCHEMBL4088.
DrugBankiDB00537. Ciprofloxacin.

Proteomic databases

PRIDEiP0C1U9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00937. ParC_type2. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR005741. TopoIV_A_Gpos.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 5 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01061. parC_Gpos. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPARC_STAAU
AccessioniPrimary (citable) accession number: P0C1U9
Secondary accession number(s): P50073, P95682, P95683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: October 5, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Phe-80 and Lys-84 confer resistance to fluoroquinolones.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.