Reviewed, UniProtKB/Swiss-Prot P0C1U7 (SLE1_STAAU)

Names and origin

Protein names

Recommended name:
    N-acetylmuramoyl-L-alanine amidase sle1
    EC=3.5.1.28

Gene names

Name:	sle1
Synonyms:	aaa

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted.

General annotation (Comments)

Function	Peptidoglycan hydrolase involved in the splitting of the septum during cell division. Binds to both alpha and beta-chains of human fibrinogen as well as fibronectin, which suggests a role in the colonization of host factor-coated material or host tissue. Also exhibits lytic activity against S.carnosus and S.aureus cells but not against M.luteus cells. Ref.1
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted. Cell surface. Ref.1
Sequence similarities	Contains 3 LysM repeats. Contains 1 peptidase C51 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation Septation Virulence
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Gene Ontology (GO)
Biological process	barrier septum formation Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 334	309	N-acetylmuramoyl-L-alanine amidase sle1		PRO_0000231620
Regions
	Repeat	29 – 71	43	LysM 1
	Repeat	93 – 135	43	LysM 2
	Repeat	160 – 202	43	LysM 3
	Domain	210 – 334	125	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	P0C1U7-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 4C1E30AD9DE61D36	FASTA	334	35,836
	10 20 30 40 50 60 MQKKVIAAII GTSAISAVAA TQANAATTHT VKPGESVWAI SNKYGISIAK LKSLNNLTSN 70 80 90 100 110 120 LIFPNQVLKV SGSSNSTSNS SRPSTNSGGG SYYTVQAGDS LSLIASKYGT TYQNIMRLNG 130 140 150 160 170 180 LNNFFIYPGQ KLKVSGTASS SNAASNSSRP STNSGGGSYY TVQAGDSLSL IASKYGTTYQ 190 200 210 220 230 240 KIMSLNGLNN FFIYPGQKLK VTGNASTNSG SATTTNRGYN TPVFSHQNLY TWGQCTYHVF 250 260 270 280 290 300 NRRAEIGKGI STYWWNANNW DNAAAADGYT IDNRPTVGSI AQTDVGYYGH VMFVERVNND 310 320 330 GSILVSEMNY SAAPGILTYR TVPAYQVNNY RYIH

References

[1]

"The multifunctional Staphylococcus aureus autolysin aaa mediates adherence to immobilized fibrinogen and fibronectin." Heilmann C., Hartleib J., Hussain M.S., Peters G. Infect. Immun. 73:4793-4802(2005) [PubMed: 16040992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: 4074.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ250906 Genomic DNA. Translation: CAC80837.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0G based on UniProtKB P23931.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.1.28. 95.
Family and domain databases
InterPro	IPR007921. CHAP. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_sg. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF01476. LysM. 3 hits. [Graphical view]
SMART	SM00257. LysM. 3 hits. [Graphical view]
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLE1_STAAU

Accession

Primary (citable) accession number: P0C1U7
Secondary accession number(s): Q33E91, Q99WD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	October 13, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)