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P0C1U4 (GUN9_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 9
EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta glucanase 9
OsCel9D
OsGLU1
Gene names
Name:GLU1
Ordered Locus Names:Os03g0329500, LOC_Os03g21210
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in cell wall assembly during cell elongation. Ref.6

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Ubiquitous. Ref.1 Ref.6

Induction

By gibberellin (GA3) and brassinosteroid. Ref.6

Disruption phenotype

Plants are dwarf with reduction in cell elongation and decrease in cellulose content. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Cellulose degradation
Polysaccharide degradation
   Cellular componentMembrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

cortical microtubule organization

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

hyperosmotic salinity response

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to cytokinin stimulus

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to nematode

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

shoot system development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

unidimensional cell growth

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cell plate

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

early endosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Endoglucanase 9
PRO_0000249286

Regions

Topological domain1 – 7474Cytoplasmic Potential
Transmembrane75 – 9521Helical; Signal-anchor for type II membrane protein; Potential
Topological domain96 – 619524Extracellular Potential

Sites

Active site5141 By similarity
Active site5621 By similarity
Active site5711 By similarity

Amino acid modifications

Glycosylation2181N-linked (GlcNAc...) Potential
Glycosylation3251N-linked (GlcNAc...) Potential
Glycosylation3461N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Glycosylation4261N-linked (GlcNAc...) Potential
Glycosylation5681N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict571R → Q in AK102748. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P0C1U4 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 7F3CCB374071162A

FASTA61968,369
        10         20         30         40         50         60 
MSMYGRDPWG GPLEICHDSA TDDDRSRNLD LDRGALSRTL DETQQSWLLA GPGDQGRKKK 

        70         80         90        100        110        120 
KYVDLGCLVV SRKLFVWTVG VLLAAAVFAG LVAGIAKAIP RHHRPPPPPD DFTVALRKAL 

       130        140        150        160        170        180 
MFFNAQKSGK LPKNNNVHWR GNSCMKDGLS DPAVGRSLVG GYYDAGDAVK FNFPAAFSMT 

       190        200        210        220        230        240 
LLSWSVIEYS AKYEAVGELG HIRDTIKWGA DYFLKTFNST ADTIDRVVMQ VGSGATSPGS 

       250        260        270        280        290        300 
TQPNDHYCWM RPEDIDYPRP VVECHACSDL AAEMAASLAA ASIVFKDNKA YSQKLVHGAT 

       310        320        330        340        350        360 
TLFKFARQNR GRYSAGGSDA AKFYNSTSYW DEFVWGGSWM YLATGNSSYL QLATHPKLAK 

       370        380        390        400        410        420 
HAGAYWGGPD YGVFSWDNKL TGAQVLLSRL RLFLSPGYPY EEILRTFHNQ TSIIMCSYLP 

       430        440        450        460        470        480 
IFKSFNRTKG GLIQLNHGRP QPLQYVVNAA FLASLYGDYL EAADTPGWYC GPHFYPIETL 

       490        500        510        520        530        540 
RNFARTQIEY ILGKNPLKMS YVVGYGNRYP KRVHHRGASI PKNGVHYGCK GGWKWRETKK 

       550        560        570        580        590        600 
PNPNIIVGAM VAGPDRHDGF KDVRKNYNYT EATLAGNAGL VAALVALSGE GHGVDKNTMF 

       610 
SAVPPMFPSP PPPPAPWKP

« Hide

References

« Hide 'large scale' references
[1]"Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is post-translationally truncated."
Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H., Nishitani K., Komae K.
Plant Cell Physiol. 47:1555-1571(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Sasanishiki.
Tissue: Root.
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[6]"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB040817 mRNA. Translation: BAF37261.1.
DP000009 Genomic DNA. Translation: ABF95745.1.
AP008209 Genomic DNA. Translation: BAF11918.1.
AK102748 mRNA. No translation available.
RefSeqNP_001050004.1. NM_001056539.1.
UniGeneOs.13910.

3D structure databases

ProteinModelPortalP0C1U4.
ModBaseSearch...

Protein-protein interaction databases

STRING39947.LOC_Os03g21210.1.

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsLOC_Os03g21210.1; LOC_Os03g21210.1; LOC_Os03g21210.
GeneID4332724.
KEGGdosa:Os03t0329500-01.
osa:4332724.

Organism-specific databases

GrameneP0C1U4.

Phylogenomic databases

eggNOGNOG257354.
HOGENOMHOG000021033.
OMAVVECHAC.
ProtClustDBCLSN2679649.

Gene expression databases

ArrayExpressP0C1U4.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. Glyco_trans_6hp. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN9_ORYSJ
AccessionPrimary (citable) accession number: P0C1U4
Secondary accession number(s): A0P890, Q10LZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: May 29, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

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