P0C1S8 (WEE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wee1-like protein kinase 2
EC=2.7.10.2

Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase

Gene names

Name:	WEE2
Synonyms:	WEE1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Nucleus Ref.1.
Tissue specificity	Expressed in testis. Ref.1
Post-translational modification	Phosphorylation leads to increase its activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	egg activation Inferred from electronic annotation. Source: Compara female meiosis Inferred from sequence or structural similarity. Source: UniProtKB female pronucleus assembly Inferred from sequence or structural similarity. Source: UniProtKB meiotic metaphase II Inferred from electronic annotation. Source: Compara meiotic prophase I Inferred from electronic annotation. Source: Compara mitosis Inferred from electronic annotation. Source: InterPro negative regulation of oocyte development Inferred from electronic annotation. Source: Compara negative regulation of oocyte maturation Inferred from sequence or structural similarity. Source: UniProtKB regulation of meiosis I Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	centrosome Inferred from direct assay. Source: HPA cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Wee1-like protein kinase 2		PRO_0000248079
Regions
	Domain	212 – 486	275	Protein kinase
	Nucleotide binding	218 – 226	9	ATP By similarity
	Coiled coil	494 – 519	26	Potential
	Motif	173 – 175	3	Nuclear localization signal By similarity
	Motif	315 – 329	15	Nuclear export signal By similarity
Sites
	Active site	339	1	Proton acceptor By similarity
	Metal binding	344	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	380	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	241	1	ATP By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity
Natural variations
	Natural variant	8	1	K → T. Ref.4 Corresponds to variant rs35672788 [ dbSNP | Ensembl ].		VAR_041304
	Natural variant	332	1	N → K in a gastric adenocarcinoma sample; somatic mutation. Ref.4		VAR_041305
	Natural variant	398	1	R → H in an ovarian mucinous carcinoma sample; somatic mutation. Ref.4		VAR_041306
	Natural variant	470	1	D → E. Ref.4 Corresponds to variant rs55901099 [ dbSNP | Ensembl ].		VAR_041307
	Natural variant	526	1	Y → D. Ref.4 Corresponds to variant rs35683659 [ dbSNP | Ensembl ].		VAR_041308
Experimental info
	Sequence conflict	351	1	M → V in AK131218. Ref.2
	Sequence conflict	529	1	H → Y in AK131218. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0C1S8 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: BC05DDFE96D87E3F	FASTA	567	62,925
	10 20 30 40 50 60 MDDKDIDKEL RQKLNFSYCE ETEIEGQKKV EESREASSQT PEKGEVQDSE AKGTPPWTPL 70 80 90 100 110 120 SNVHELDTSS EKDKESPDQI LRTPVSHPLK CPETPAQPDS RSKLLPSDSP STPKTMLSRL 130 140 150 160 170 180 VISPTGKLPS RGPKHLKLTP APLKDEMTSL ALVNINPFTP ESYKKLFLQS GGKRKIRGDL 190 200 210 220 230 240 EEAGPEEGKG GLPAKRCVLR ETNMASRYEK EFLEVEKIGV GEFGTVYKCI KRLDGCVYAI 250 260 270 280 290 300 KRSMKTFTEL SNENSALHEV YAHAVLGHHP HVVRYYSSWA EDDHMIIQNE YCNGGSLQAA 310 320 330 340 350 360 ISENTKSGNH FEEPKLKDIL LQISLGLNYI HNSSMVHLDI KPSNIFICHK MQSESSGVIE 370 380 390 400 410 420 EVENEADWFL SANVMYKIGD LGHATSINKP KVEEGDSRFL ANEILQEDYR HLPKADIFAL 430 440 450 460 470 480 GLTIAVAAGA ESLPTNGAAW HHIRKGNFPD VPQELSESFS SLLKNMIQPD AEQRPSAAAL 490 500 510 520 530 540 ARNTVLRPSL GKTEELQQQL NLEKFKTATL ERELREAQQA QSPQGYTHHG DTGVSGTHTG 550 560 SRSTKRLVGG KSARSSSFTS GEREPLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of human Wee1B, a new member of the Wee1 family of Cdk-inhibitory kinases." Nakanishi M., Ando H., Watanabe N., Kitamura K., Ito K., Okayama H., Miyamoto T., Agui T., Sasaki M. Genes Cells 5:839-847(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-8; LYS-332; HIS-398; GLU-470 AND ASP-526.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK131218 mRNA. No translation available. AC004918 Genomic DNA. No translation available.
IPI	IPI00745231.
RefSeq	NP_001099028.1. NM_001105558.1.
UniGene	Hs.657927.
3D structure databases
ProteinModelPortal	P0C1S8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000380675.
PTM databases
PhosphoSite	P0C1S8.
Polymorphism databases
DMDM	254763343.
Proteomic databases
PaxDb	P0C1S8.
PRIDE	P0C1S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000397541; ENSP00000380675; ENSG00000214102. ENST00000574659; ENSP00000459392; ENSG00000263042.
GeneID	494551.
KEGG	hsa:494551.
UCSC	uc003vwn.2. human.
Organism-specific databases
CTD	494551.
GeneCards	GC07P141409.
H-InvDB	HIX0007154. HIX0033848.
HGNC	HGNC:19684. WEE2.
HPA	HPA027418.
MIM	614084. gene.
neXtProt	NX_P0C1S8.
PharmGKB	PA134909633.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000004824.
HOVERGEN	HBG005050.
InParanoid	P0C1S8.
KO	K06632.
OMA	ANVMYKI.
OrthoDB	EOG4FJ88X.
Enzyme and pathway databases
SignaLink	P0C1S8.
Gene expression databases
ArrayExpress	P0C1S8.
Bgee	P0C1S8.
CleanEx	HS_WEE2.
Genevestigator	P0C1S8.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. IPR017164. Wee1-like_protein_kinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P0C1S8.
ChEMBL	CHEMBL5095.
GenomeRNAi	494551.
NextBio	111889.
SOURCE	Search...

Entry information

Entry name

WEE2_HUMAN

Accession

Primary (citable) accession number: P0C1S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 28, 2009
Last modified:	May 29, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.