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P0C1S8

- WEE2_HUMAN

UniProt

P0C1S8 - WEE2_HUMAN

Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei241 – 2411ATPPROSITE-ProRule annotation
    Active sitei339 – 3391Proton acceptorPROSITE-ProRule annotation
    Metal bindingi344 – 3441Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi380 – 3801Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi218 – 2269ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. female meiotic division Source: UniProtKB
    2. female pronucleus assembly Source: UniProtKB
    3. mitotic nuclear division Source: InterPro
    4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
    5. negative regulation of oocyte maturation Source: UniProtKB
    6. regulation of meiosis I Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiP0C1S8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase 2 (EC:2.7.10.2)
    Alternative name(s):
    Wee1-like protein kinase 1B
    Wee1B kinase
    Gene namesi
    Name:WEE2
    Synonyms:WEE1B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:19684. WEE2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134909633.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 567567Wee1-like protein kinase 2PRO_0000248079Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei76 – 761PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation leads to increase its activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP0C1S8.
    PRIDEiP0C1S8.

    PTM databases

    PhosphoSiteiP0C1S8.

    Expressioni

    Tissue specificityi

    Expressed in testis.1 Publication

    Gene expression databases

    BgeeiP0C1S8.
    CleanExiHS_WEE2.
    GenevestigatoriP0C1S8.

    Organism-specific databases

    HPAiHPA027418.

    Interactioni

    Protein-protein interaction databases

    BioGridi138978. 1 interaction.
    STRINGi9606.ENSP00000380675.

    Structurei

    3D structure databases

    ProteinModelPortaliP0C1S8.
    SMRiP0C1S8. Positions 206-549.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini212 – 486275Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili494 – 51926Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi173 – 1753Nuclear localization signalBy similarity
    Motifi315 – 32915Nuclear export signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000004824.
    HOVERGENiHBG005050.
    InParanoidiP0C1S8.
    KOiK06632.
    OMAiICHKMQS.
    PhylomeDBiP0C1S8.
    TreeFamiTF101088.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C1S8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDKDIDKEL RQKLNFSYCE ETEIEGQKKV EESREASSQT PEKGEVQDSE    50
    AKGTPPWTPL SNVHELDTSS EKDKESPDQI LRTPVSHPLK CPETPAQPDS 100
    RSKLLPSDSP STPKTMLSRL VISPTGKLPS RGPKHLKLTP APLKDEMTSL 150
    ALVNINPFTP ESYKKLFLQS GGKRKIRGDL EEAGPEEGKG GLPAKRCVLR 200
    ETNMASRYEK EFLEVEKIGV GEFGTVYKCI KRLDGCVYAI KRSMKTFTEL 250
    SNENSALHEV YAHAVLGHHP HVVRYYSSWA EDDHMIIQNE YCNGGSLQAA 300
    ISENTKSGNH FEEPKLKDIL LQISLGLNYI HNSSMVHLDI KPSNIFICHK 350
    MQSESSGVIE EVENEADWFL SANVMYKIGD LGHATSINKP KVEEGDSRFL 400
    ANEILQEDYR HLPKADIFAL GLTIAVAAGA ESLPTNGAAW HHIRKGNFPD 450
    VPQELSESFS SLLKNMIQPD AEQRPSAAAL ARNTVLRPSL GKTEELQQQL 500
    NLEKFKTATL ERELREAQQA QSPQGYTHHG DTGVSGTHTG SRSTKRLVGG 550
    KSARSSSFTS GEREPLH 567
    Length:567
    Mass (Da):62,925
    Last modified:July 28, 2009 - v2
    Checksum:iBC05DDFE96D87E3F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti351 – 3511M → V in AK131218. (PubMed:14702039)Curated
    Sequence conflicti529 – 5291H → Y in AK131218. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti8 – 81K → T.1 Publication
    Corresponds to variant rs35672788 [ dbSNP | Ensembl ].
    VAR_041304
    Natural varianti332 – 3321N → K in a gastric adenocarcinoma sample; somatic mutation. 1 Publication
    VAR_041305
    Natural varianti398 – 3981R → H in an ovarian mucinous carcinoma sample; somatic mutation. 1 Publication
    VAR_041306
    Natural varianti470 – 4701D → E.1 Publication
    Corresponds to variant rs55901099 [ dbSNP | Ensembl ].
    VAR_041307
    Natural varianti526 – 5261Y → D.1 Publication
    Corresponds to variant rs35683659 [ dbSNP | Ensembl ].
    VAR_041308

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK131218 mRNA. No translation available.
    AC004918 Genomic DNA. No translation available.
    CCDSiCCDS43660.1.
    RefSeqiNP_001099028.1. NM_001105558.1.
    UniGeneiHs.657927.

    Genome annotation databases

    EnsembliENST00000397541; ENSP00000380675; ENSG00000214102.
    ENST00000574659; ENSP00000459392; ENSG00000263042.
    GeneIDi494551.
    KEGGihsa:494551.
    UCSCiuc003vwn.2. human.

    Polymorphism databases

    DMDMi254763343.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK131218 mRNA. No translation available.
    AC004918 Genomic DNA. No translation available.
    CCDSi CCDS43660.1.
    RefSeqi NP_001099028.1. NM_001105558.1.
    UniGenei Hs.657927.

    3D structure databases

    ProteinModelPortali P0C1S8.
    SMRi P0C1S8. Positions 206-549.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 138978. 1 interaction.
    STRINGi 9606.ENSP00000380675.

    Chemistry

    BindingDBi P0C1S8.
    ChEMBLi CHEMBL5095.
    GuidetoPHARMACOLOGYi 2279.

    PTM databases

    PhosphoSitei P0C1S8.

    Polymorphism databases

    DMDMi 254763343.

    Proteomic databases

    PaxDbi P0C1S8.
    PRIDEi P0C1S8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000397541 ; ENSP00000380675 ; ENSG00000214102 .
    ENST00000574659 ; ENSP00000459392 ; ENSG00000263042 .
    GeneIDi 494551.
    KEGGi hsa:494551.
    UCSCi uc003vwn.2. human.

    Organism-specific databases

    CTDi 494551.
    GeneCardsi GC07P141409.
    H-InvDB HIX0007154.
    HIX0033848.
    HGNCi HGNC:19684. WEE2.
    HPAi HPA027418.
    MIMi 614084. gene.
    neXtProti NX_P0C1S8.
    PharmGKBi PA134909633.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000004824.
    HOVERGENi HBG005050.
    InParanoidi P0C1S8.
    KOi K06632.
    OMAi ICHKMQS.
    PhylomeDBi P0C1S8.
    TreeFami TF101088.

    Enzyme and pathway databases

    SignaLinki P0C1S8.

    Miscellaneous databases

    GenomeRNAii 494551.
    NextBioi 111889.
    PROi P0C1S8.
    SOURCEi Search...

    Gene expression databases

    Bgeei P0C1S8.
    CleanExi HS_WEE2.
    Genevestigatori P0C1S8.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of human Wee1B, a new member of the Wee1 family of Cdk-inhibitory kinases."
      Nakanishi M., Ando H., Watanabe N., Kitamura K., Ito K., Okayama H., Miyamoto T., Agui T., Sasaki M.
      Genes Cells 5:839-847(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    3. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-8; LYS-332; HIS-398; GLU-470 AND ASP-526.

    Entry informationi

    Entry nameiWEE2_HUMAN
    AccessioniPrimary (citable) accession number: P0C1S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3