P0C1P9 (DPO3_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III PolC-type
Short name=PolIII
EC=2.7.7.7

Gene names

Name:

polC

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	1435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity. HAMAP-Rule MF_00356
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_00356
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. PolC subfamily. Contains 1 exonuclease domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1435	1435	DNA polymerase III PolC-type HAMAP-Rule MF_00356		PRO_0000204591
Regions
	Domain	420 – 576	157	Exonuclease

Sequences

	Sequence		Length	Mass (Da)
	P0C1P9 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 59CA6C672B56981F	FASTA	1,435	162,477
	10 20 30 40 50 60 MTEQQKFKVL ADQIKISNQL DAEILNSGEL TRIDVSNKNR TWEFHITLPQ FLAHEDYLLF 70 80 90 100 110 120 INAIEQEFKD IANVTCRFTV TNGTNQDEHA IKYFGHCIDQ TALSPKVKGQ LKQKKLIMSG 130 140 150 160 170 180 KVLKVMVSND IERNHFDKAC NGSLIKAFRN CGFDIDKIIF ETNDNDQEQN LASLEAHIQE 190 200 210 220 230 240 EDEQSARLAT EKLEKMKAEK AKQQDNKQSA VDKCQIGKPI QIENIKPIES IIEEEFKVAI 250 260 270 280 290 300 EGVIFDINLK ELKSGRHIVE IKVTDYTDSL VLKMFTRKNK DDLEHFKALS VGKWVRAQGR 310 320 330 340 350 360 IEEDTFIRDL VMMMSDIEEI KKATKKDKAE EKRVEFHLHT AMSQMDGIPN IGAYVKQAAD 370 380 390 400 410 420 WGHPAIAVTD HNVVQAFPDA HAAAEKHGIK MIYGMEGMLV DDGVPIAYKP QDVVLKDATY 430 440 450 460 470 480 VVFDVETTGL SNQYDKIIEL AAVKVHNGEI IDKFERFSNP HERLSETIIN LTHITDDMLV 490 500 510 520 530 540 DAPEIEEVLT EFKEWVGDAI FVAHNASFDM GFIDTGYERL GFGPSTNGVI DTLELSRTIN 550 560 570 580 590 600 TEYGKHGLNF LAKKYGVELT QHHRAIYDTE ATAYIFIKMV QQMKELGVLN HNEINKKLSN 610 620 630 640 650 660 EDAYKRARPS HVTLIVQNQQ GLKNLFKIVS ASLVKYFYRT PRIPRSLLDE YREGLLVGTA 670 680 690 700 710 720 CDEGELFTAV MQKDQSQVEK IAKYYDFIEI QPPALYQDLI DRELIRDTET LHEIYQRLIH 730 740 750 760 770 780 AGDTAGIPVI ATGNAHYLFE HDGIARKILI ASQPGNPLNR STLPEAHFRT TDEMLNEFHF 790 800 810 820 830 840 LGEEKAHEIV VKNTNELADR IERVVPIKDE LYTPRMEGAN EEIRELSYAN ARKLYGEDLP 850 860 870 880 890 900 QIVIDRLEKE LKSIIGNGFA VIYLISQRLV KKSLDDGYLV GSRGSVGSSF VATMTEITEV 910 920 930 940 950 960 NPLPPHYICP NCKTSEFFND GSVGSGFDLP DKTCETCGAP LIKEGQDIPF EKFLGFKGDK 970 980 990 1000 1010 1020 VPDIDLNFSG EYQPNAHNYT KVLFGEDKVF RAGTIGTVAE KTAFGYVKGY LNDQGIHKRG 1030 1040 1050 1060 1070 1080 AEIDRLVKGC TGVKATTGQH PGGIIVVPDY MDIYDFTPIQ YPADDQNSAW MTTHFDFHSI 1090 1100 1110 1120 1130 1140 HDNVLKLDIL GHDDPTMIRM LQDLSGIDPK TIPVDDKEVM QIFSTPESLG VTEDEILCKT 1150 1160 1170 1180 1190 1200 GTFGVPNSDR IRRQMLEDTK PTTFSELVQI SGLSHGTDVW LGNAQELIKT GICDLSSVIG 1210 1220 1230 1240 1250 1260 CRDDIMVYLM YAGLEPSMAF KIMESVRKGK GLTEEMIETM KENEVPDWYL DSCLKIKYIF 1270 1280 1290 1300 1310 1320 PKAHAAAYVL MAVRIAYFKV HHPLYYYASY FTIRASDFDL ITMIKDKTSI RNTVKDMYSR 1330 1340 1350 1360 1370 1380 YMDLGKKEKD VLTVLEIMNE MAHRGYRMQP ISLEKSQAFE FIIEGDTLIP PFISVPGLGE 1390 1400 1410 1420 1430 NVAKRIVEAR DDGPFLSKED LNKKAGLYQK IIEYLDELGS LPNLPDKAQL SIFDM

References

[1]	"Characterization and overexpression of the gene encoding Staphylococcus aureus DNA polymerase III." Pacitti D.F., Barnes M.H., Li D., Brown N.C. Gene 165:51-56(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IP8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86727 Genomic DNA. Translation: BAA13160.1. Z48003 Genomic DNA. Translation: CAA88043.1.
PIR	S52267.
3D structure databases
ProteinModelPortal	P0C1P9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.5.140. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_00356. DNApol_PolC.
InterPro	IPR011708. DNA_pol3_alpha. IPR024754. DNA_polC-like_N. IPR023223. DNA_polIII_IA3_alpha-helix. IPR006054. DnaQ. IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR012340. NA-bd_OB-fold. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR006308. PolC_gram_pos. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF11490. DNA_pol3_alph_N. 1 hit. PF07733. DNA_pol3_alpha. 2 hits. PF02811. PHP. 1 hit. PF00929. RNase_T. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00573. dnaq. 1 hit. TIGR01405. polC_Gram_pos. 1 hit.
ProtoNet	Search...
Other
BindingDB	P0C1P9.
ChEMBL	CHEMBL4910.

Entry information

Entry name

DPO3_STAAU

Accession

Primary (citable) accession number: P0C1P9
Secondary accession number(s): P68851 , Q53665, Q57110, Q9F1J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program