Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0C1H3 (H2B1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone H2B 1/2/3/4/6
Alternative name(s):
H2B I
H2B II
H2B III
H2B IV
H2B VI
Gene names
Name:H2B-I
AND
Name:H2B-II
AND
Name:H2B-III
AND
Name:H2B-IV
AND
Name:H2B-VI
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length126 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Ref.11

Has broad-spectrum antibacterial activity. May be important in the antimicrobial defenses of chick reproductive system during follicle development in the ovary and egg formation in the oviduct. Ref.11

Subunit structure

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location

Nucleus. Chromosome.

Post-translational modification

Monoubiquitination of Lys-121 by the BRE1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation By similarity.

Phosphorylated on Ser-15 during apoptosis; which facilitates apoptotic chromatin condensation. Ref.9

Sequence similarities

Belongs to the histone H2B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6 Ref.7
Chain2 – 126125Histone H2B 1/2/3/4/6
PRO_0000071846

Amino acid modifications

Modified residue61N6-acetyllysine Ref.10
Modified residue131N6-acetyllysine Ref.10
Modified residue151Phosphoserine Ref.9
Modified residue161N6-acetyllysine Ref.10
Modified residue211N6-acetyllysine Ref.10
Cross-link121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Probable

Experimental info

Sequence conflict141G → A in CAA28745. Ref.2
Sequence conflict361E → A in CAA28747. Ref.2
Sequence conflict621I → S AA sequence Ref.6
Sequence conflict1231T → I in CAA28751. Ref.2

Secondary structure

........... 126
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0C1H3 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 908F039A02A3400D

FASTA12613,922
        10         20         30         40         50         60 
MPEPAKSAPA PKKGSKKAVT KTQKKGDKKR KKSRKESYSI YVYKVLKQVH PDTGISSKAM 

        70         80         90        100        110        120 
GIMNSFVNDI FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT 


KYTSSK 

« Hide

References

[1]"Complete nucleotide sequence of a chicken H2B histone gene."
Grandy D.K., Engel J.D., Dodgson J.B.
J. Biol. Chem. 257:8577-8580(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structure and organization of the chicken H2B histone gene family."
Grandy D.K., Dodgson J.B.
Nucleic Acids Res. 15:1063-1080(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: White leghorn.
Tissue: Blood.
[3]"Nucleotide sequence of a member of the chicken H2B histone-encoding gene family."
Nakayama T., Setoguchi Y.
Gene 98:299-300(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Organization of the chicken histone genes in a major gene cluster and generation of an almost complete set of the core histone protein sequences."
Takami Y., Higashio M., Fukuoka T., Takechi S., Nakayama T.
DNA Res. 3:95-99(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NOMENCLATURE.
Strain: White leghorn.
[5]"Conservation of histone H2A/H2B intergene regions: a role for the H2B specific element in divergent transcription."
Sturm R.A., Dalton S., Wells J.R.E.
Nucleic Acids Res. 16:8571-8586(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The complete amino-acid sequence of histone H2B from erythrocytes of the adult domestic fowl Gallus domesticus."
van Helden P., Strickland W.N., Strickland M., von Holt C.
Biochim. Biophys. Acta 703:17-20(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-126.
[7]"Histone H2B variants from the erythrocytes of an amphibian, a reptile and a bird."
van Helden P., Strickland W.N., Brandt W.F., von Holt C.
Biochim. Biophys. Acta 533:278-281(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-30 AND 62-90.
Tissue: Erythrocyte.
[8]"Ubiquitinated histone H2B is preferentially located in transcriptionally active chromatin."
Nickel B.E., Allis C.D., Davie J.R.
Biochemistry 28:958-963(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION.
[9]"Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase."
Cheung W.L., Ajiro K., Samejima K., Kloc M., Cheung P., Mizzen C.A., Beeser A., Etkin L.D., Chernoff J., Earnshaw W.C., Allis C.D.
Cell 113:507-517(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-15.
[10]"Acetylation of histone H2B mirrors that of H4 and H3 at the chicken beta-globin locus but not at housekeeping genes."
Myers F.A., Chong W., Evans D.R., Thorne A.W., Crane-Robinson C.
J. Biol. Chem. 278:36315-36322(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT LYS-6; LYS-13; LYS-16 AND LYS-21.
[11]"Antimicrobial proteins in chicken reproductive system."
Silphaduang U., Hincke M.T., Nys Y., Mine Y.
Biochem. Biophys. Res. Commun. 340:648-655(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN ANTIBIOTIC, IDENTIFICATION BY MASS SPECTROMETRY.
[12]"The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix."
Arents G., Burlingame R.W., Wang B.-C., Love W.E., Moudrianakis E.N.
Proc. Natl. Acad. Sci. U.S.A. 88:10148-10152(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00415 Genomic DNA. Translation: CAA23706.1.
X05094 Genomic DNA. Translation: CAA28745.1.
X05095 Genomic DNA. Translation: CAA28746.1.
X05096 Genomic DNA. Translation: CAA28747.1.
X05097 Genomic DNA. Translation: CAA28748.1.
X05098 Genomic DNA. Translation: CAA28749.1.
X05100 Genomic DNA. Translation: CAA28751.1.
X57263 Genomic DNA. Translation: CAA40537.1.
X07763 Genomic DNA. Translation: CAA30590.1.
X07766 Genomic DNA. Translation: CAA30596.1.
PIRB26399.
HSCH22. S14510.
RefSeqNP_001073188.1. NM_001079720.1.
XP_001232985.1. XM_001232984.2.
XP_001233227.1. XM_001233226.3.
XP_001233337.1. XM_001233336.3.
UniGeneGga.39843.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AROX-ray2.10B/F1-126[»]
ProteinModelPortalP0C1H3.
SMRP0C1H3. Positions 19-126.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid679231. 3 interactions.
IntActP0C1H3. 1 interaction.
STRING9031.ENSGALP00000037300.

Proteomic databases

PaxDbP0C1H3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000021778; ENSGALP00000021742; ENSGALG00000027174.
ENSGALT00000042841; ENSGALP00000042437; ENSGALG00000027594.
ENSGALT00000043224; ENSGALP00000042501; ENSGALG00000027571.
GeneID417956.
769973.
770188.
770267.
KEGGgga:417956.
gga:769973.
gga:770188.
gga:770267.

Organism-specific databases

CTD417956.
769973.
770188.
770267.

Phylogenomic databases

eggNOGNOG303883.
GeneTreeENSGT00720000108413.
HOGENOMHOG000231213.
HOVERGENHBG007774.
InParanoidP0C1H3.
KOK11252.
PhylomeDBP0C1H3.
TreeFamTF300212.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERPTHR23428. PTHR23428. 1 hit.
PfamPF00125. Histone. 1 hit.
[Graphical view]
PRINTSPR00621. HISTONEH2B.
SMARTSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMSSF47113. SSF47113. 1 hit.
PROSITEPS00357. HISTONE_H2B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0C1H3.
NextBio20821182.

Entry information

Entry nameH2B1_CHICK
AccessionPrimary (citable) accession number: P0C1H3
Secondary accession number(s): P02279, Q92067
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references