Reviewed, UniProtKB/Swiss-Prot P0C1G8 (PSA4A_ORYSJ)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-4-1
    EC=3.4.25.1
Alternative name(s):
    20S proteasome alpha subunit C
    20S proteasome subunit alpha-3

Gene names

Name:	PAC1
Ordered Locus Names:	Os06g0176000, LOC_Os06g07878
ORF Names:	P0015E04.21, OsJ_019497
AND
Ordered Locus Names:	Os06g0177100, LOC_Os06g07978
ORF Names:	P0015E04.34

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Proteasome subunit alpha type-4-1		PRO_0000124112

Sequences

	Sequence		Length	Mass (Da)
	P0C1G8-1 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 00FDAD801A97ABD4	FASTA	250	27,044
	10 20 30 40 50 60 MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGN AGSALGVLAA DGVVLVGEKK VTSKLLQTSR 70 80 90 100 110 120 SAEKMYKIDS HLACAVAGIM SDANILLNTA RLHAQRYALS YQEPIPVEQL VQSLCDTKQG 130 140 150 160 170 180 YTQFGGLRPF GVSFLFAGWD KHHGFQLYMS DPSGNYSGWK AAAVGANSQA AQSMLKQDYR 190 200 210 220 230 240 DGMTREEAVA LALKVLSKTM DSTSLTAEKL ELAEVFLQPG TGEVQYQVCS PEAMGKLLAK 250 AGLSQPAPEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structural features of the 20S proteasome subunits of rice (Oryza sativa)." Sassa H., Oguchi S., Inoue T., Hirano H. Gene 250:61-66(2000) [PubMed: 10854779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB026560 mRNA. Translation: BAA96831.1. AP002069 Genomic DNA. Translation: BAA95822.1. AP002069 Genomic DNA. Translation: BAA95832.1. AP008212 Genomic DNA. Translation: BAF18867.1. CM000143 Genomic DNA. Translation: EAZ36014.1.
RefSeq	NP_001056953.1.
UniGene	Os.88355
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P23638.
ModBase	Search...
Genome annotation databases
GeneID	4340285.
KEGG	osa:4340285.
Organism-specific databases
Gramene	Q9LE92.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA4A_ORYSJ

Accession

Primary (citable) accession number: P0C1G8
Secondary accession number(s): Q0DE56, Q9LE92, Q9XIZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	October 13, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)