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Protein

Malate dehydrogenase

Gene

mdh

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251NADBy similarity
Binding sitei72 – 721SubstratePROSITE-ProRule annotation
Binding sitei78 – 781SubstratePROSITE-ProRule annotation
Binding sitei85 – 851NADBy similarity
Binding sitei110 – 1101SubstratePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 126NADBy similarity
Nucleotide bindingi108 – 1103NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›135›135Malate dehydrogenasePRO_0000113310Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272620.KPN_03644.

Structurei

3D structure databases

ProteinModelPortaliP0C1G4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF00056. Ldh_1_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

P0C1G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVLXAAG GIQALALLLK TSLYDIAPVT PGVAVDLSHI PTDVKIKGFS
60 70 80 90 100
GEDATPALEG ADVVLISAGV ARKPGMDRSD LFNVNAGIVK NLVQQIAKTC
110 120 130
PQACIGIITN PVNTTVAIAA EVLKKAGVYD KNKLF
Length:135
Mass (Da):13,935
Last modified:June 13, 2006 - v1
Checksum:iE8379B36BB9BD94B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi21 – 222Curated
Non-terminal residuei135 – 1351

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti63 – 631V → I in strain: ES694:2.
Natural varianti99 – 991T → N in strain: UD711:2 and VA680:2.
Natural varianti107 – 1071I → V in strain: SB1, SB31, SB95, SB96, SO661:2, OR803:4, UD711:2, JO757:2, JO436:1, KK427:2, UD827:1, UD892:1 and VA680:2.
Natural varianti127 – 1271G → D in strain: ES694:2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ635372 Genomic DNA. Translation: CAG25783.1.
AJ635373 Genomic DNA. Translation: CAG25784.1.
AJ635374 Genomic DNA. Translation: CAG25785.1.
AJ635375 Genomic DNA. Translation: CAG25786.1.
AJ635376 Genomic DNA. Translation: CAG25787.1.
AJ635377 Genomic DNA. Translation: CAG25788.1.
AJ635378 Genomic DNA. Translation: CAG25789.1.
AJ635380 Genomic DNA. Translation: CAG25791.1.
AJ635381 Genomic DNA. Translation: CAG25792.1.
AJ635382 Genomic DNA. Translation: CAG25793.1.
AJ635383 Genomic DNA. Translation: CAG25794.1.
AJ635384 Genomic DNA. Translation: CAG25795.1.
AJ635385 Genomic DNA. Translation: CAG25796.1.
AJ635386 Genomic DNA. Translation: CAG25797.1.
AJ635387 Genomic DNA. Translation: CAG25798.1.
AJ635388 Genomic DNA. Translation: CAG25799.1.
AJ635389 Genomic DNA. Translation: CAG25800.1.
AJ635390 Genomic DNA. Translation: CAG25801.1.
AJ635392 Genomic DNA. Translation: CAG25803.1.
AJ635393 Genomic DNA. Translation: CAG25804.1.
AJ635394 Genomic DNA. Translation: CAG25805.1.
AJ635395 Genomic DNA. Translation: CAG25806.1.
AJ635396 Genomic DNA. Translation: CAG25807.1.
AJ635397 Genomic DNA. Translation: CAG25808.1.
AJ635398 Genomic DNA. Translation: CAG25809.1.
AJ635399 Genomic DNA. Translation: CAG25810.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ635372 Genomic DNA. Translation: CAG25783.1.
AJ635373 Genomic DNA. Translation: CAG25784.1.
AJ635374 Genomic DNA. Translation: CAG25785.1.
AJ635375 Genomic DNA. Translation: CAG25786.1.
AJ635376 Genomic DNA. Translation: CAG25787.1.
AJ635377 Genomic DNA. Translation: CAG25788.1.
AJ635378 Genomic DNA. Translation: CAG25789.1.
AJ635380 Genomic DNA. Translation: CAG25791.1.
AJ635381 Genomic DNA. Translation: CAG25792.1.
AJ635382 Genomic DNA. Translation: CAG25793.1.
AJ635383 Genomic DNA. Translation: CAG25794.1.
AJ635384 Genomic DNA. Translation: CAG25795.1.
AJ635385 Genomic DNA. Translation: CAG25796.1.
AJ635386 Genomic DNA. Translation: CAG25797.1.
AJ635387 Genomic DNA. Translation: CAG25798.1.
AJ635388 Genomic DNA. Translation: CAG25799.1.
AJ635389 Genomic DNA. Translation: CAG25800.1.
AJ635390 Genomic DNA. Translation: CAG25801.1.
AJ635392 Genomic DNA. Translation: CAG25803.1.
AJ635393 Genomic DNA. Translation: CAG25804.1.
AJ635394 Genomic DNA. Translation: CAG25805.1.
AJ635395 Genomic DNA. Translation: CAG25806.1.
AJ635396 Genomic DNA. Translation: CAG25807.1.
AJ635397 Genomic DNA. Translation: CAG25808.1.
AJ635398 Genomic DNA. Translation: CAG25809.1.
AJ635399 Genomic DNA. Translation: CAG25810.1.

3D structure databases

ProteinModelPortaliP0C1G4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_03644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF00056. Ldh_1_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure."
    Charnock C.
    J. Bacteriol. 179:4066-4070(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-21.
  2. "Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae."
    Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S.
    Antimicrob. Agents Chemother. 48:2400-2408(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-135.
    Strain: 1976E, ATCC 11296, ATCC 13883 / DSM 30104 / JCM 1662 / NBRC 14940 / NCIMB 13281 / NCTC 9633, ES694:2, HU653:4, JO436:1, JO757:2, KK427:2, MA207:1, OR803:4, OR95:2, SB1, SB18, SB30, SB31, SB59, SB95, SB96, SO661:2, UD1001:4, UD580:1, UD711:2, UD827:1, UD890:1, UD892:1 and VA680:2.

Entry informationi

Entry nameiMDH_KLEPN
AccessioniPrimary (citable) accession number: P0C1G4
Secondary accession number(s): P80535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: June 24, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.