P0C1G4 (MDH_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:

mdh

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	135 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›135	›135	Malate dehydrogenase HAMAP-Rule MF_01516		PRO_0000113310
Regions
	Nucleotide binding	7 – 12	6	NAD By similarity
	Nucleotide binding	108 – 110	3	NAD By similarity
Sites
	Binding site	25	1	NAD By similarity
	Binding site	72	1	Substrate By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	85	1	NAD By similarity
	Binding site	110	1	Substrate By similarity
Natural variations
	Natural variant	63	1	V → I in strain: ES694:2.
	Natural variant	99	1	T → N in strain: UD711:2 and VA680:2.
	Natural variant	107	1	I → V in strain: SB1, SB31, SB95, SB96, SO661:2, OR803:4, UD711:2, JO757:2, JO436:1, KK427:2, UD827:1, UD892:1 and VA680:2.
	Natural variant	127	1	G → D in strain: ES694:2.
Experimental info
	Non-adjacent residues	21 – 22	2
	Non-terminal residue	135	1

Sequences

	Sequence		Length	Mass (Da)
	P0C1G4 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: E8379B36BB9BD94B	FASTA	135	13,935
	10 20 30 40 50 60 MKVAVLXAAG GIQALALLLK TSLYDIAPVT PGVAVDLSHI PTDVKIKGFS GEDATPALEG 70 80 90 100 110 120 ADVVLISAGV ARKPGMDRSD LFNVNAGIVK NLVQQIAKTC PQACIGIITN PVNTTVAIAA 130 EVLKKAGVYD KNKLF

References

[1]

"Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure." Charnock C. J. Bacteriol. 179:4066-4070(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21.

[2]

"Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae." Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S. Antimicrob. Agents Chemother. 48:2400-2408(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-135. Strain: 1976E, ATCC 11296, ATCC 13883 / DSM 30104 / JCM 1662 / NBRC 14940 / NCIMB 13281 / NCTC 9633, ES694:2, HU653:4, JO436:1, JO757:2, KK427:2, MA207:1, OR803:4, OR95:2, SB1, SB18, SB30, SB31, SB59, SB95, SB96, SO661:2, UD1001:4, UD580:1, UD711:2, UD827:1, UD890:1, UD892:1 and VA680:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ635372 Genomic DNA. Translation: CAG25783.1. AJ635373 Genomic DNA. Translation: CAG25784.1. AJ635374 Genomic DNA. Translation: CAG25785.1. AJ635375 Genomic DNA. Translation: CAG25786.1. AJ635376 Genomic DNA. Translation: CAG25787.1. AJ635377 Genomic DNA. Translation: CAG25788.1. AJ635378 Genomic DNA. Translation: CAG25789.1. AJ635380 Genomic DNA. Translation: CAG25791.1. AJ635381 Genomic DNA. Translation: CAG25792.1. AJ635382 Genomic DNA. Translation: CAG25793.1. AJ635383 Genomic DNA. Translation: CAG25794.1. AJ635384 Genomic DNA. Translation: CAG25795.1. AJ635385 Genomic DNA. Translation: CAG25796.1. AJ635386 Genomic DNA. Translation: CAG25797.1. AJ635387 Genomic DNA. Translation: CAG25798.1. AJ635388 Genomic DNA. Translation: CAG25799.1. AJ635389 Genomic DNA. Translation: CAG25800.1. AJ635390 Genomic DNA. Translation: CAG25801.1. AJ635392 Genomic DNA. Translation: CAG25803.1. AJ635393 Genomic DNA. Translation: CAG25804.1. AJ635394 Genomic DNA. Translation: CAG25805.1. AJ635395 Genomic DNA. Translation: CAG25806.1. AJ635396 Genomic DNA. Translation: CAG25807.1. AJ635397 Genomic DNA. Translation: CAG25808.1. AJ635398 Genomic DNA. Translation: CAG25809.1. AJ635399 Genomic DNA. Translation: CAG25810.1.
3D structure databases
ProteinModelPortal	P0C1G4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF00056. Ldh_1_N. 1 hit. [Graphical view]
PROSITE	PS00068. MDH. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_KLEPN

Accession

Primary (citable) accession number: P0C1G4
Secondary accession number(s): P80535

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program