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P0C1G4 (MDH_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length135 AA.
Sequence statusFragments.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›135›135Malate dehydrogenase
PRO_0000113310

Regions

Nucleotide binding7 – 126NAD By similarity
Nucleotide binding108 – 1103NAD By similarity

Sites

Binding site251NAD By similarity
Binding site721Substrate By similarity
Binding site781Substrate By similarity
Binding site851NAD By similarity
Binding site1101Substrate By similarity

Natural variations

Natural variant631V → I in strain: ES694:2.
Natural variant991T → N in strain: UD711:2 and VA680:2.
Natural variant1071I → V in strain: SB1, SB31, SB95, SB96, SO661:2, OR803:4, UD711:2, JO757:2, JO436:1, KK427:2, UD827:1, UD892:1 and VA680:2.
Natural variant1271G → D in strain: ES694:2.

Experimental info

Non-adjacent residues21 – 222
Non-terminal residue1351

Sequences

Sequence LengthMass (Da)Tools
P0C1G4 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: E8379B36BB9BD94B

FASTA13513,935
        10         20         30         40         50         60 
MKVAVLXAAG GIQALALLLK TSLYDIAPVT PGVAVDLSHI PTDVKIKGFS GEDATPALEG 

        70         80         90        100        110        120 
ADVVLISAGV ARKPGMDRSD LFNVNAGIVK NLVQQIAKTC PQACIGIITN PVNTTVAIAA 

       130 
EVLKKAGVYD KNKLF 

« Hide

References

[1]"Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure."
Charnock C.
J. Bacteriol. 179:4066-4070(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-21.
[2]"Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae."
Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S.
Antimicrob. Agents Chemother. 48:2400-2408(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-135.
Strain: 1976E, ATCC 11296, ATCC 13883 / DSM 30104 / JCM 1662 / NBRC 14940 / NCIMB 13281 / NCTC 9633, ES694:2, HU653:4, JO436:1, JO757:2, KK427:2, MA207:1, OR803:4, OR95:2, SB1, SB18, SB30, SB31, SB59, SB95, SB96, SO661:2, UD1001:4, UD580:1, UD711:2, UD827:1, UD890:1, UD892:1 and VA680:2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ635372 Genomic DNA. Translation: CAG25783.1.
AJ635373 Genomic DNA. Translation: CAG25784.1.
AJ635374 Genomic DNA. Translation: CAG25785.1.
AJ635375 Genomic DNA. Translation: CAG25786.1.
AJ635376 Genomic DNA. Translation: CAG25787.1.
AJ635377 Genomic DNA. Translation: CAG25788.1.
AJ635378 Genomic DNA. Translation: CAG25789.1.
AJ635380 Genomic DNA. Translation: CAG25791.1.
AJ635381 Genomic DNA. Translation: CAG25792.1.
AJ635382 Genomic DNA. Translation: CAG25793.1.
AJ635383 Genomic DNA. Translation: CAG25794.1.
AJ635384 Genomic DNA. Translation: CAG25795.1.
AJ635385 Genomic DNA. Translation: CAG25796.1.
AJ635386 Genomic DNA. Translation: CAG25797.1.
AJ635387 Genomic DNA. Translation: CAG25798.1.
AJ635388 Genomic DNA. Translation: CAG25799.1.
AJ635389 Genomic DNA. Translation: CAG25800.1.
AJ635390 Genomic DNA. Translation: CAG25801.1.
AJ635392 Genomic DNA. Translation: CAG25803.1.
AJ635393 Genomic DNA. Translation: CAG25804.1.
AJ635394 Genomic DNA. Translation: CAG25805.1.
AJ635395 Genomic DNA. Translation: CAG25806.1.
AJ635396 Genomic DNA. Translation: CAG25807.1.
AJ635397 Genomic DNA. Translation: CAG25808.1.
AJ635398 Genomic DNA. Translation: CAG25809.1.
AJ635399 Genomic DNA. Translation: CAG25810.1.

3D structure databases

ProteinModelPortalP0C1G4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF00056. Ldh_1_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_KLEPN
AccessionPrimary (citable) accession number: P0C1G4
Secondary accession number(s): P80535
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: February 19, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families