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P0C1G4

- MDH_KLEPN

UniProt

P0C1G4 - MDH_KLEPN

Protein

Malate dehydrogenase

Gene

mdh

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251NADBy similarity
    Binding sitei72 – 721SubstratePROSITE-ProRule annotation
    Binding sitei78 – 781SubstratePROSITE-ProRule annotation
    Binding sitei85 – 851NADBy similarity
    Binding sitei110 – 1101SubstratePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 126NADBy similarity
    Nucleotide bindingi108 – 1103NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›135›135Malate dehydrogenasePRO_0000113310Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C1G4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR001236. Lactate/malate_DH_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF00056. Ldh_1_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    P0C1G4-1 [UniParc]FASTAAdd to Basket

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    MKVAVLXAAG GIQALALLLK TSLYDIAPVT PGVAVDLSHI PTDVKIKGFS    50
    GEDATPALEG ADVVLISAGV ARKPGMDRSD LFNVNAGIVK NLVQQIAKTC 100
    PQACIGIITN PVNTTVAIAA EVLKKAGVYD KNKLF 135
    Length:135
    Mass (Da):13,935
    Last modified:June 13, 2006 - v1
    Checksum:iE8379B36BB9BD94B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi21 – 222Curated
    Non-terminal residuei135 – 1351

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti63 – 631V → I in strain: ES694:2.
    Natural varianti99 – 991T → N in strain: UD711:2 and VA680:2.
    Natural varianti107 – 1071I → V in strain: SB1, SB31, SB95, SB96, SO661:2, OR803:4, UD711:2, JO757:2, JO436:1, KK427:2, UD827:1, UD892:1 and VA680:2.
    Natural varianti127 – 1271G → D in strain: ES694:2.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ635372 Genomic DNA. Translation: CAG25783.1.
    AJ635373 Genomic DNA. Translation: CAG25784.1.
    AJ635374 Genomic DNA. Translation: CAG25785.1.
    AJ635375 Genomic DNA. Translation: CAG25786.1.
    AJ635376 Genomic DNA. Translation: CAG25787.1.
    AJ635377 Genomic DNA. Translation: CAG25788.1.
    AJ635378 Genomic DNA. Translation: CAG25789.1.
    AJ635380 Genomic DNA. Translation: CAG25791.1.
    AJ635381 Genomic DNA. Translation: CAG25792.1.
    AJ635382 Genomic DNA. Translation: CAG25793.1.
    AJ635383 Genomic DNA. Translation: CAG25794.1.
    AJ635384 Genomic DNA. Translation: CAG25795.1.
    AJ635385 Genomic DNA. Translation: CAG25796.1.
    AJ635386 Genomic DNA. Translation: CAG25797.1.
    AJ635387 Genomic DNA. Translation: CAG25798.1.
    AJ635388 Genomic DNA. Translation: CAG25799.1.
    AJ635389 Genomic DNA. Translation: CAG25800.1.
    AJ635390 Genomic DNA. Translation: CAG25801.1.
    AJ635392 Genomic DNA. Translation: CAG25803.1.
    AJ635393 Genomic DNA. Translation: CAG25804.1.
    AJ635394 Genomic DNA. Translation: CAG25805.1.
    AJ635395 Genomic DNA. Translation: CAG25806.1.
    AJ635396 Genomic DNA. Translation: CAG25807.1.
    AJ635397 Genomic DNA. Translation: CAG25808.1.
    AJ635398 Genomic DNA. Translation: CAG25809.1.
    AJ635399 Genomic DNA. Translation: CAG25810.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ635372 Genomic DNA. Translation: CAG25783.1 .
    AJ635373 Genomic DNA. Translation: CAG25784.1 .
    AJ635374 Genomic DNA. Translation: CAG25785.1 .
    AJ635375 Genomic DNA. Translation: CAG25786.1 .
    AJ635376 Genomic DNA. Translation: CAG25787.1 .
    AJ635377 Genomic DNA. Translation: CAG25788.1 .
    AJ635378 Genomic DNA. Translation: CAG25789.1 .
    AJ635380 Genomic DNA. Translation: CAG25791.1 .
    AJ635381 Genomic DNA. Translation: CAG25792.1 .
    AJ635382 Genomic DNA. Translation: CAG25793.1 .
    AJ635383 Genomic DNA. Translation: CAG25794.1 .
    AJ635384 Genomic DNA. Translation: CAG25795.1 .
    AJ635385 Genomic DNA. Translation: CAG25796.1 .
    AJ635386 Genomic DNA. Translation: CAG25797.1 .
    AJ635387 Genomic DNA. Translation: CAG25798.1 .
    AJ635388 Genomic DNA. Translation: CAG25799.1 .
    AJ635389 Genomic DNA. Translation: CAG25800.1 .
    AJ635390 Genomic DNA. Translation: CAG25801.1 .
    AJ635392 Genomic DNA. Translation: CAG25803.1 .
    AJ635393 Genomic DNA. Translation: CAG25804.1 .
    AJ635394 Genomic DNA. Translation: CAG25805.1 .
    AJ635395 Genomic DNA. Translation: CAG25806.1 .
    AJ635396 Genomic DNA. Translation: CAG25807.1 .
    AJ635397 Genomic DNA. Translation: CAG25808.1 .
    AJ635398 Genomic DNA. Translation: CAG25809.1 .
    AJ635399 Genomic DNA. Translation: CAG25810.1 .

    3D structure databases

    ProteinModelPortali P0C1G4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR001236. Lactate/malate_DH_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure."
      Charnock C.
      J. Bacteriol. 179:4066-4070(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-21.
    2. "Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae."
      Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S.
      Antimicrob. Agents Chemother. 48:2400-2408(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-135.
      Strain: 1976E, ATCC 11296, ATCC 13883 / DSM 30104 / JCM 1662 / NBRC 14940 / NCIMB 13281 / NCTC 9633, ES694:2, HU653:4, JO436:1, JO757:2, KK427:2, MA207:1, OR803:4, OR95:2, SB1, SB18, SB30, SB31, SB59, SB95, SB96, SO661:2, UD1001:4, UD580:1, UD711:2, UD827:1, UD890:1, UD892:1 and VA680:2.

    Entry informationi

    Entry nameiMDH_KLEPN
    AccessioniPrimary (citable) accession number: P0C1G4
    Secondary accession number(s): P80535
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3